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Conference Paper: Suberoylanilide Hydroxamic Acid Mediates Enhanced Apoptosis In Nasopharyngeal Carcinoma Through Induction Of Epstein-Barr Virus Lytic Cycle And Activation Of Caspase-3

TitleSuberoylanilide Hydroxamic Acid Mediates Enhanced Apoptosis In Nasopharyngeal Carcinoma Through Induction Of Epstein-Barr Virus Lytic Cycle And Activation Of Caspase-3
Authors
Issue Date2010
PublisherInternational Association for Research on Epstein-Barr Virus & Associated Diseases. The Conference abstracts' web site is located at: https://www.bcm.edu/ebvassociation/downloads/EBV2010.pdf
Citation
The 14th Biennial Conference of the International Association for Research on Epstein-Barr Virus & Associated Diseases, University of Birmingham, UK., 4-7 September 2010, p. 241, abstract no. P113 How to Cite?
AbstractEpstein-Barr virus (EBV) persists in tightly latent form in nasopharyngeal carcinoma (NPC) evading immune surveillance. Induction of EBV lytic cycle will lead to expression of a much larger number of viral proteins which may serve as potential therapeutic targets for the cancer. We previously showed that suberoylanilide hydroxamic acid (SAHA), a FDAapproved histone deacetylase inhibitor, could strongly induce viral lytic cycle in EBV-positive gastric carcinoma cells. In this study, we tested SAHA for its ability to induce EBV lytic cycle in NPC cell lines including HONE1-EBV, HK1-EBV, HA and NA. Following treatment with 5- 10 μM SAHA, increased replication of EBV DNA (8-70 folds), expression of immediate early (Zta and Rta), early (BMRF1) and late (gp350/220) viral lytic proteins (up to 60% of cells expressing BMRF1) and production of infectious viral particles were observed in all four NPC cell lines. Furthermore, enhanced killing of EBV-positive NPC cells, compared with that of EBV-negative counterparts, was demonstrated by MTT [3-(4,5-Dimethylthiazol-2-yl)-2,5- diphenyltetrazolium bromide] assay. Higher percentage of EBV-positive than EBV-negative NPC cells expressing annexin V suggested that enhanced killing of EBV-positive NPC cells was related to apoptosis. Increased expression of cleaved poly(ADP-ribose) polymerase (PARP) and cleaved caspase-3 in EBV-positive compared with that in EBV-negative NPC cells indicated that enhanced apoptosis was mediated by activation of caspase-3. In conclusion, SAHA is a potent inducing agent of EBV lytic cycle and can mediate enhanced apoptosis of NPC cells.
DescriptionPoster session: Nasopharyngeal carcinoma and gastric cancer
Persistent Identifierhttp://hdl.handle.net/10722/197288

 

DC FieldValueLanguage
dc.contributor.authorHui, KFen_US
dc.contributor.authorTsang, CMen_US
dc.contributor.authorTsao, GSWen_US
dc.contributor.authorChiang, AKSen_US
dc.date.accessioned2014-05-23T02:35:34Z-
dc.date.available2014-05-23T02:35:34Z-
dc.date.issued2010en_US
dc.identifier.citationThe 14th Biennial Conference of the International Association for Research on Epstein-Barr Virus & Associated Diseases, University of Birmingham, UK., 4-7 September 2010, p. 241, abstract no. P113en_US
dc.identifier.urihttp://hdl.handle.net/10722/197288-
dc.descriptionPoster session: Nasopharyngeal carcinoma and gastric cancer-
dc.description.abstractEpstein-Barr virus (EBV) persists in tightly latent form in nasopharyngeal carcinoma (NPC) evading immune surveillance. Induction of EBV lytic cycle will lead to expression of a much larger number of viral proteins which may serve as potential therapeutic targets for the cancer. We previously showed that suberoylanilide hydroxamic acid (SAHA), a FDAapproved histone deacetylase inhibitor, could strongly induce viral lytic cycle in EBV-positive gastric carcinoma cells. In this study, we tested SAHA for its ability to induce EBV lytic cycle in NPC cell lines including HONE1-EBV, HK1-EBV, HA and NA. Following treatment with 5- 10 μM SAHA, increased replication of EBV DNA (8-70 folds), expression of immediate early (Zta and Rta), early (BMRF1) and late (gp350/220) viral lytic proteins (up to 60% of cells expressing BMRF1) and production of infectious viral particles were observed in all four NPC cell lines. Furthermore, enhanced killing of EBV-positive NPC cells, compared with that of EBV-negative counterparts, was demonstrated by MTT [3-(4,5-Dimethylthiazol-2-yl)-2,5- diphenyltetrazolium bromide] assay. Higher percentage of EBV-positive than EBV-negative NPC cells expressing annexin V suggested that enhanced killing of EBV-positive NPC cells was related to apoptosis. Increased expression of cleaved poly(ADP-ribose) polymerase (PARP) and cleaved caspase-3 in EBV-positive compared with that in EBV-negative NPC cells indicated that enhanced apoptosis was mediated by activation of caspase-3. In conclusion, SAHA is a potent inducing agent of EBV lytic cycle and can mediate enhanced apoptosis of NPC cells.-
dc.languageengen_US
dc.publisherInternational Association for Research on Epstein-Barr Virus & Associated Diseases. The Conference abstracts' web site is located at: https://www.bcm.edu/ebvassociation/downloads/EBV2010.pdf-
dc.relation.ispartofBiennial Conference of the International Association for Research on Epstein-Barr Virus & Associated Diseasesen_US
dc.titleSuberoylanilide Hydroxamic Acid Mediates Enhanced Apoptosis In Nasopharyngeal Carcinoma Through Induction Of Epstein-Barr Virus Lytic Cycle And Activation Of Caspase-3en_US
dc.typeConference_Paperen_US
dc.identifier.emailTsang, CM: anna0226@graduate.hku.hken_US
dc.identifier.emailTsao, GSW: gswtsao@hkucc.hku.hken_US
dc.identifier.emailChiang, AKS: chiangak@hkucc.hku.hken_US
dc.identifier.authorityTsao, GSW=rp00399en_US
dc.identifier.authorityChiang, AKS=rp00403en_US
dc.identifier.hkuros183701en_US
dc.identifier.spage241, abstract no. P113-
dc.identifier.epage241, abstract no. P113-
dc.publisher.placeUnited Kingdom-

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