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Conference Paper: Suberoylanilide Hydroxamic Acid Mediates Enhanced Apoptosis In Nasopharyngeal Carcinoma Through Induction Of Epstein-Barr Virus Lytic Cycle And Activation Of Caspase-3
Title | Suberoylanilide Hydroxamic Acid Mediates Enhanced Apoptosis In Nasopharyngeal Carcinoma Through Induction Of Epstein-Barr Virus Lytic Cycle And Activation Of Caspase-3 |
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Authors | |
Issue Date | 2010 |
Publisher | International Association for Research on Epstein-Barr Virus & Associated Diseases. The Conference abstracts' web site is located at: https://www.bcm.edu/ebvassociation/downloads/EBV2010.pdf |
Citation | The 14th Biennial Conference of the International Association for Research on Epstein-Barr Virus and Associated Diseases, University of Birmingham, UK., 4-7 September 2010, p. 241, abstract no. P113 How to Cite? |
Abstract | Epstein-Barr virus (EBV) persists in tightly latent form in nasopharyngeal carcinoma (NPC)
evading immune surveillance. Induction of EBV lytic cycle will lead to expression of a much
larger number of viral proteins which may serve as potential therapeutic targets for the
cancer. We previously showed that suberoylanilide hydroxamic acid (SAHA), a FDAapproved
histone deacetylase inhibitor, could strongly induce viral lytic cycle in EBV-positive
gastric carcinoma cells. In this study, we tested SAHA for its ability to induce EBV lytic cycle
in NPC cell lines including HONE1-EBV, HK1-EBV, HA and NA. Following treatment with 5-
10 μM SAHA, increased replication of EBV DNA (8-70 folds), expression of immediate early
(Zta and Rta), early (BMRF1) and late (gp350/220) viral lytic proteins (up to 60% of cells
expressing BMRF1) and production of infectious viral particles were observed in all four NPC
cell lines. Furthermore, enhanced killing of EBV-positive NPC cells, compared with that of
EBV-negative counterparts, was demonstrated by MTT [3-(4,5-Dimethylthiazol-2-yl)-2,5-
diphenyltetrazolium bromide] assay. Higher percentage of EBV-positive than EBV-negative
NPC cells expressing annexin V suggested that enhanced killing of EBV-positive NPC cells
was related to apoptosis. Increased expression of cleaved poly(ADP-ribose) polymerase
(PARP) and cleaved caspase-3 in EBV-positive compared with that in EBV-negative NPC
cells indicated that enhanced apoptosis was mediated by activation of caspase-3. In
conclusion, SAHA is a potent inducing agent of EBV lytic cycle and can mediate enhanced
apoptosis of NPC cells. |
Description | Poster session: Nasopharyngeal carcinoma and gastric cancer |
Persistent Identifier | http://hdl.handle.net/10722/197288 |
DC Field | Value | Language |
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dc.contributor.author | Hui, KF | en_US |
dc.contributor.author | Tsang, CM | en_US |
dc.contributor.author | Tsao, GSW | en_US |
dc.contributor.author | Chiang, AKS | en_US |
dc.date.accessioned | 2014-05-23T02:35:34Z | - |
dc.date.available | 2014-05-23T02:35:34Z | - |
dc.date.issued | 2010 | en_US |
dc.identifier.citation | The 14th Biennial Conference of the International Association for Research on Epstein-Barr Virus and Associated Diseases, University of Birmingham, UK., 4-7 September 2010, p. 241, abstract no. P113 | en_US |
dc.identifier.uri | http://hdl.handle.net/10722/197288 | - |
dc.description | Poster session: Nasopharyngeal carcinoma and gastric cancer | - |
dc.description.abstract | Epstein-Barr virus (EBV) persists in tightly latent form in nasopharyngeal carcinoma (NPC) evading immune surveillance. Induction of EBV lytic cycle will lead to expression of a much larger number of viral proteins which may serve as potential therapeutic targets for the cancer. We previously showed that suberoylanilide hydroxamic acid (SAHA), a FDAapproved histone deacetylase inhibitor, could strongly induce viral lytic cycle in EBV-positive gastric carcinoma cells. In this study, we tested SAHA for its ability to induce EBV lytic cycle in NPC cell lines including HONE1-EBV, HK1-EBV, HA and NA. Following treatment with 5- 10 μM SAHA, increased replication of EBV DNA (8-70 folds), expression of immediate early (Zta and Rta), early (BMRF1) and late (gp350/220) viral lytic proteins (up to 60% of cells expressing BMRF1) and production of infectious viral particles were observed in all four NPC cell lines. Furthermore, enhanced killing of EBV-positive NPC cells, compared with that of EBV-negative counterparts, was demonstrated by MTT [3-(4,5-Dimethylthiazol-2-yl)-2,5- diphenyltetrazolium bromide] assay. Higher percentage of EBV-positive than EBV-negative NPC cells expressing annexin V suggested that enhanced killing of EBV-positive NPC cells was related to apoptosis. Increased expression of cleaved poly(ADP-ribose) polymerase (PARP) and cleaved caspase-3 in EBV-positive compared with that in EBV-negative NPC cells indicated that enhanced apoptosis was mediated by activation of caspase-3. In conclusion, SAHA is a potent inducing agent of EBV lytic cycle and can mediate enhanced apoptosis of NPC cells. | - |
dc.language | eng | en_US |
dc.publisher | International Association for Research on Epstein-Barr Virus & Associated Diseases. The Conference abstracts' web site is located at: https://www.bcm.edu/ebvassociation/downloads/EBV2010.pdf | - |
dc.relation.ispartof | Biennial Conference of the International Association for Research on EBV & Associated Diseases | en_US |
dc.title | Suberoylanilide Hydroxamic Acid Mediates Enhanced Apoptosis In Nasopharyngeal Carcinoma Through Induction Of Epstein-Barr Virus Lytic Cycle And Activation Of Caspase-3 | en_US |
dc.type | Conference_Paper | en_US |
dc.identifier.email | Tsang, CM: anna0226@graduate.hku.hk | en_US |
dc.identifier.email | Tsao, GSW: gswtsao@hkucc.hku.hk | en_US |
dc.identifier.email | Chiang, AKS: chiangak@hkucc.hku.hk | en_US |
dc.identifier.authority | Tsao, GSW=rp00399 | en_US |
dc.identifier.authority | Chiang, AKS=rp00403 | en_US |
dc.identifier.hkuros | 183701 | en_US |
dc.identifier.spage | 241, abstract no. P113 | - |
dc.identifier.epage | 241, abstract no. P113 | - |
dc.publisher.place | United Kingdom | - |