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Article: Importin-mediated retrograde transport of CREB2 from distal processes to the nucleus in neurons

TitleImportin-mediated retrograde transport of CREB2 from distal processes to the nucleus in neurons
Authors
Issue Date2008
PublisherNational Academy of Sciences. The Journal's web site is located at http://www.pnas.org
Citation
Proceedings of the National Academy of Sciences, 2008, v. 105 n. 44, p. 17175-17180 How to Cite?
AbstractSignals received at distal synapses of neurons must be conveyed to the nucleus to initiate the changes in transcription that underlie long-lasting synaptic plasticity. The presence of importin nuclear transporters and of select transcription factors at synapses raises the possibility that importins directly transport transcription factors from synapse to nucleus to modulate gene expression. Here, we show that cyclic AMP response element binding protein 2 (CREB2)/activating transcription factor 4 (ATF4), a transcriptional repressor that modulates long-term synaptic plasticity and memory, localizes to distal dendrites of rodent hippocampal neurons and neurites of Aplysia sensory neurons (SNs) and binds to specific importin alpha isoforms. Binding of CREB2 to importin alpha is required for its transport from distal dendrites to the soma and for its translocation into the nucleus. CREB2 accumulates in the nucleus during long-term depression (LTD) but not long-term potentiation of rodent hippocampal synapses, and during LTD but not long-term facilitation (LTF) of Aplysia sensory-motor synapses. Time-lapse microscopy of CREB2 tagged with a photoconvertible fluorescent protein further reveals retrograde transport of CREB2 from distal neurites to the nucleus of Aplysia SN during phenylalanine-methionine-arginine-phenylalanine-amide (FMRFamide)-induced LTD. Together, our findings indicate that CREB2 is a novel cargo of importin alpha that translocates from distal synaptic sites to the nucleus after stimuli that induce LTD of neuronal synapses.
Persistent Identifierhttp://hdl.handle.net/10722/196730
ISSN
2015 Impact Factor: 9.423
2015 SCImago Journal Rankings: 6.883
PubMed Central ID
ISI Accession Number ID

 

DC FieldValueLanguage
dc.contributor.authorLai, KO-
dc.contributor.authorZhao, Y-
dc.contributor.authorToh, HC-
dc.contributor.authorMartin, KC-
dc.date.accessioned2014-04-25T02:24:42Z-
dc.date.available2014-04-25T02:24:42Z-
dc.date.issued2008-
dc.identifier.citationProceedings of the National Academy of Sciences, 2008, v. 105 n. 44, p. 17175-17180-
dc.identifier.issn0027-8424-
dc.identifier.urihttp://hdl.handle.net/10722/196730-
dc.description.abstractSignals received at distal synapses of neurons must be conveyed to the nucleus to initiate the changes in transcription that underlie long-lasting synaptic plasticity. The presence of importin nuclear transporters and of select transcription factors at synapses raises the possibility that importins directly transport transcription factors from synapse to nucleus to modulate gene expression. Here, we show that cyclic AMP response element binding protein 2 (CREB2)/activating transcription factor 4 (ATF4), a transcriptional repressor that modulates long-term synaptic plasticity and memory, localizes to distal dendrites of rodent hippocampal neurons and neurites of Aplysia sensory neurons (SNs) and binds to specific importin alpha isoforms. Binding of CREB2 to importin alpha is required for its transport from distal dendrites to the soma and for its translocation into the nucleus. CREB2 accumulates in the nucleus during long-term depression (LTD) but not long-term potentiation of rodent hippocampal synapses, and during LTD but not long-term facilitation (LTF) of Aplysia sensory-motor synapses. Time-lapse microscopy of CREB2 tagged with a photoconvertible fluorescent protein further reveals retrograde transport of CREB2 from distal neurites to the nucleus of Aplysia SN during phenylalanine-methionine-arginine-phenylalanine-amide (FMRFamide)-induced LTD. Together, our findings indicate that CREB2 is a novel cargo of importin alpha that translocates from distal synaptic sites to the nucleus after stimuli that induce LTD of neuronal synapses.-
dc.languageeng-
dc.publisherNational Academy of Sciences. The Journal's web site is located at http://www.pnas.org-
dc.relation.ispartofProceedings of the National Academy of Sciences-
dc.subject.meshCell Nucleus - metabolism-
dc.subject.meshCyclic AMP Response Element-Binding Protein - metabolism-
dc.subject.meshNerve Tissue Proteins - metabolism-
dc.subject.meshRepressor Proteins - metabolism-
dc.subject.meshSensory Receptor Cells - metabolism-
dc.titleImportin-mediated retrograde transport of CREB2 from distal processes to the nucleus in neuronsen_US
dc.typeArticleen_US
dc.identifier.emailLai, KO: laiko@hku.hk-
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1073/pnas.0803906105-
dc.identifier.pmid18957537-
dc.identifier.pmcidPMC2579397-
dc.identifier.volume105-
dc.identifier.issue44-
dc.identifier.spage17175-
dc.identifier.epage17180-
dc.identifier.isiWOS:000260913800068-
dc.publisher.placeUnited States-
dc.identifier.f10001138064-

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