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Article: Single-site sonoporation disrupts actin cytoskeleton organization

TitleSingle-site sonoporation disrupts actin cytoskeleton organization
Authors
Issue Date2014
PublisherThe Royal Society. The Journal's web site is located at http://publishing.royalsociety.org/index.cfm?page=1572
Citation
Journal of the Royal Society. Interface, 2014, v. 11 n. 95, p. article no. 20140071 How to Cite?
AbstractSonoporation is based upon an ultrasound-microbubble cavitation routine that physically punctures the plasma membrane on a transient basis. Over such process, the actin cytoskeleton may be disrupted in tandem since this network of subcellular filaments is physically interconnected with the plasma membrane. Here, by performing confocal fluorescence imaging of single-site sonoporation episodes induced by ultrasound-triggered collapse of a single targeted microbubble, we directly observed immediate rupturing of filamentary actin (F-actin) at the sonoporation site (cell type: ZR-75-30; ultrasound frequency: 1 MHz; peak negative pressure: 0.45 MPa; pulse duration: 30 cycles; bubble diameter: 2-4 m). Also, through conducting a structure tensor analysis, we observed further disassembly of the F-actin network over the next 60 min after the onset of sonoporation. The extent of F-actin disruption was found to be more substantial in cells with higher uptake of sonoporation tracer. Commensurate with this process, cytoplasmic accumulation of globular actin (G-actin) was evident in sonoporated cells, and in turn the G:F-actin ratio was increased in a trend similar to drug-induced (cytochalasin D) actin depolymerization. These results demonstrate that sonoporation is not solely a membrane-level phenomenon: organization of the actin cytoskeleton is concomitantly perturbed.
Persistent Identifierhttp://hdl.handle.net/10722/195913
ISSN
2015 Impact Factor: 3.818
2015 SCImago Journal Rankings: 1.622
ISI Accession Number ID

 

DC FieldValueLanguage
dc.contributor.authorChen, Xen_US
dc.contributor.authorLeow, RSen_US
dc.contributor.authorHu, Yen_US
dc.contributor.authorWan, JMFen_US
dc.contributor.authorYu, ACHen_US
dc.date.accessioned2014-03-21T02:19:28Z-
dc.date.available2014-03-21T02:19:28Z-
dc.date.issued2014-
dc.identifier.citationJournal of the Royal Society. Interface, 2014, v. 11 n. 95, p. article no. 20140071en_US
dc.identifier.issn1742-5689-
dc.identifier.urihttp://hdl.handle.net/10722/195913-
dc.description.abstractSonoporation is based upon an ultrasound-microbubble cavitation routine that physically punctures the plasma membrane on a transient basis. Over such process, the actin cytoskeleton may be disrupted in tandem since this network of subcellular filaments is physically interconnected with the plasma membrane. Here, by performing confocal fluorescence imaging of single-site sonoporation episodes induced by ultrasound-triggered collapse of a single targeted microbubble, we directly observed immediate rupturing of filamentary actin (F-actin) at the sonoporation site (cell type: ZR-75-30; ultrasound frequency: 1 MHz; peak negative pressure: 0.45 MPa; pulse duration: 30 cycles; bubble diameter: 2-4 m). Also, through conducting a structure tensor analysis, we observed further disassembly of the F-actin network over the next 60 min after the onset of sonoporation. The extent of F-actin disruption was found to be more substantial in cells with higher uptake of sonoporation tracer. Commensurate with this process, cytoplasmic accumulation of globular actin (G-actin) was evident in sonoporated cells, and in turn the G:F-actin ratio was increased in a trend similar to drug-induced (cytochalasin D) actin depolymerization. These results demonstrate that sonoporation is not solely a membrane-level phenomenon: organization of the actin cytoskeleton is concomitantly perturbed.en_US
dc.languageengen_US
dc.publisherThe Royal Society. The Journal's web site is located at http://publishing.royalsociety.org/index.cfm?page=1572-
dc.relation.ispartofJournal of the Royal Society. Interfaceen_US
dc.titleSingle-site sonoporation disrupts actin cytoskeleton organizationen_US
dc.typeArticleen_US
dc.identifier.emailWan, JMF: jmfwan@hku.hken_US
dc.identifier.emailYu, ACH: alfred.yu@hku.hken_US
dc.identifier.authorityWan, JMF=rp00798en_US
dc.identifier.authorityYu, ACH=rp00657en_US
dc.identifier.doi10.1098/rsif.2014.0071en_US
dc.identifier.pmid24671936-
dc.identifier.hkuros228302en_US
dc.identifier.volume11-
dc.identifier.issue95-
dc.identifier.spagearticle no. 20140071-
dc.identifier.epagearticle no. 20140071-
dc.identifier.isiWOS:000335639900018-
dc.publisher.placeUnited Kingdom-

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