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Article: Caries arresting effect of silver diamine fluoride on dentine carious lesion with S. mutans and L. acidophilus dual-species cariogenic biofilm

TitleCaries arresting effect of silver diamine fluoride on dentine carious lesion with S. mutans and L. acidophilus dual-species cariogenic biofilm
Authors
Issue Date2013
PublisherMedicina Oral SL. The Journal's web site is located at http://scielo.isciii.es/revistas/medicor/iaboutj.htm
Citation
Medicina Oral, Patologia Oral y Cirugia Bucal, 2013, v. 18 n. 6, p. e824-e831 How to Cite?
AbstractOBJECTIVES: This in vitro study investigated the effects of silver diamine fluoride (SDF) on dentine carious lesion with cariogenic biofilm. STUDY DESIGN: Thirty human dentine blocks were inoculated with Streptococcus mutans and Lactobacillus acidophilus dual-species biofilm to create carious lesion. They were equally divided into test and control group to receive topical application of SDF and water. After incubation anaerobically using micro-well plate at 37oC for 7 days, the biofilms were evaluated for kinetics, morphology and viability by colony forming units (CFU), scanning electron microscopy (SEM), and confocal microscopy (CLSM), respectively. The carious lesion underwent crystal characteristics analysis, evaluation of the changes in chemical structure and density of collagen fibrils using x-ray diffraction (XRD), Fourier transform infrared spectroscopy (FTIR) and immune-labeling. RESULTS: The log CFU of S. mutans and L. acidophilus in the test group was significantly lower than control group. SEM and CLSM showed confluent biofilm in control group, but not in test group. XRD showed the loss of crystallinity of dentine due to the dissolution of hydroxyapatite crystal structure in test group was less than control group. FTIR showed that log [Amide I: HPO42-] for test vs. control group was 0.31±0.10 vs. 0.57±0.13 (p<0.05). The gold-labeling density in test vs. control group was 8.54±2.44/µm2 vs. 12.91±4.24/µm2 (p=0.04). CONCLUSIONS: SDF had antimicrobial activity against the cariogenic biofilms and reduced demineralization of dentine.
Persistent Identifierhttp://hdl.handle.net/10722/195908
ISSN
2011 Impact Factor: 1.13
2015 SCImago Journal Rankings: 0.542
PubMed Central ID
ISI Accession Number ID

 

DC FieldValueLanguage
dc.contributor.authorMei, Len_US
dc.contributor.authorChu, CHen_US
dc.contributor.authorLow, KHen_US
dc.contributor.authorChe, CMen_US
dc.contributor.authorLo, ECMen_US
dc.date.accessioned2014-03-21T02:17:20Z-
dc.date.available2014-03-21T02:17:20Z-
dc.date.issued2013en_US
dc.identifier.citationMedicina Oral, Patologia Oral y Cirugia Bucal, 2013, v. 18 n. 6, p. e824-e831en_US
dc.identifier.issn1698-4447-
dc.identifier.urihttp://hdl.handle.net/10722/195908-
dc.description.abstractOBJECTIVES: This in vitro study investigated the effects of silver diamine fluoride (SDF) on dentine carious lesion with cariogenic biofilm. STUDY DESIGN: Thirty human dentine blocks were inoculated with Streptococcus mutans and Lactobacillus acidophilus dual-species biofilm to create carious lesion. They were equally divided into test and control group to receive topical application of SDF and water. After incubation anaerobically using micro-well plate at 37oC for 7 days, the biofilms were evaluated for kinetics, morphology and viability by colony forming units (CFU), scanning electron microscopy (SEM), and confocal microscopy (CLSM), respectively. The carious lesion underwent crystal characteristics analysis, evaluation of the changes in chemical structure and density of collagen fibrils using x-ray diffraction (XRD), Fourier transform infrared spectroscopy (FTIR) and immune-labeling. RESULTS: The log CFU of S. mutans and L. acidophilus in the test group was significantly lower than control group. SEM and CLSM showed confluent biofilm in control group, but not in test group. XRD showed the loss of crystallinity of dentine due to the dissolution of hydroxyapatite crystal structure in test group was less than control group. FTIR showed that log [Amide I: HPO42-] for test vs. control group was 0.31±0.10 vs. 0.57±0.13 (p<0.05). The gold-labeling density in test vs. control group was 8.54±2.44/µm2 vs. 12.91±4.24/µm2 (p=0.04). CONCLUSIONS: SDF had antimicrobial activity against the cariogenic biofilms and reduced demineralization of dentine.en_US
dc.languageengen_US
dc.publisherMedicina Oral SL. The Journal's web site is located at http://scielo.isciii.es/revistas/medicor/iaboutj.htmen_US
dc.relation.ispartofMedicina Oral, Patologia Oral y Cirugia Bucalen_US
dc.titleCaries arresting effect of silver diamine fluoride on dentine carious lesion with S. mutans and L. acidophilus dual-species cariogenic biofilmen_US
dc.typeArticleen_US
dc.identifier.emailMei, L: mei1123@hku.hken_US
dc.identifier.emailChu, CH: chchu@hku.hken_US
dc.identifier.emailLow, KH: khlow@hku.hken_US
dc.identifier.emailChe, CM: cmche@hku.hken_US
dc.identifier.emailLo, ECM: hrdplcm@hkucc.hku.hken_US
dc.identifier.authorityMei, L=rp01840en_US
dc.identifier.authorityChu, CH=rp00022en_US
dc.identifier.authorityChe, CM=rp00670en_US
dc.identifier.authorityLo, ECM=rp00015en_US
dc.description.naturelink_to_OA_fulltext-
dc.identifier.doi10.4317/medoral.18831-
dc.identifier.pmid23722131-
dc.identifier.pmcidPMC3854072-
dc.identifier.hkuros228331en_US
dc.identifier.hkuros226788-
dc.identifier.hkuros227928-
dc.identifier.volume18en_US
dc.identifier.issue6-
dc.identifier.spagee824en_US
dc.identifier.epagee831en_US
dc.identifier.isiWOS:000326790500001-
dc.publisher.placeSpain-

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