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Article: Bornyl caffeate induces apoptosis in human breast cancer MCF-7 cells via the ROS- and JNK-mediated pathways

TitleBornyl caffeate induces apoptosis in human breast cancer MCF-7 cells via the ROS- and JNK-mediated pathways
Authors
Issue Date2014
PublisherNature Publishing Group. The Journal's web site is located at http://www.nature.com/aps/index.html
Citation
Acta Pharmacologica Sinica, 2014, v. 35 n. 1, p. 113-123 How to Cite?
AbstractAIM: The purpose of the present study was to investigate the anticancer activity of bornyl caffeate in the human breast cancer cell line MCF-7. METHODS: The cell viability was determined using the MTT assay, and apoptosis was initially defined by monitoring the morphology of the cell nuclei and staining an early apoptotic biomarker with Annexin V-FITC. The mitochondrial membrane potential was visualized by JC-1 under fluorescence microscopy, whereas intracellular reactive oxygen species (ROS) were assessed by flow cytometry. The expression of apoptosis-associated proteins was determined by Western blotting analysis. RESULTS: Bornyl caffeate induced apoptosis in MCF-7 cells in a dose- and time-dependent manner. Consistently, bornyl caffeate increased Bax and decreased Bcl-xl, resulting in the disruption of MMP and subsequent activation of caspase-3. Moreover, bornyl caffeate triggered the formation of ROS and the activation of the mitogen-activated protein (MAP) kinases p38 and c-Jun N-terminal kinase (JNK). Antioxidants attenuated the activation of MAP kinase p38 but barely affected the activation of JNK. Importantly, the cytotoxicity of bornyl caffeate was partially attenuated by scavenging ROS and inhibited by MAP kinases and caspases. CONCLUSION: The present study demonstrated that bornyl caffeate induced apoptosis in the cancer cell line MCF-7 via activating the ROS- and JNK-mediated pathways. Thus, bornyl caffeate may be a potential anticancer lead compound.
Persistent Identifierhttp://hdl.handle.net/10722/195719
ISSN
2015 Impact Factor: 3.166
2015 SCImago Journal Rankings: 1.161
ISI Accession Number ID

 

DC FieldValueLanguage
dc.contributor.authorYang, Cen_US
dc.contributor.authorPei, WJen_US
dc.contributor.authorZhao, Jen_US
dc.contributor.authorCheng, Yen_US
dc.contributor.authorZheng, XHen_US
dc.contributor.authorRong, Jen_US
dc.date.accessioned2014-03-07T04:32:37Z-
dc.date.available2014-03-07T04:32:37Z-
dc.date.issued2014en_US
dc.identifier.citationActa Pharmacologica Sinica, 2014, v. 35 n. 1, p. 113-123en_US
dc.identifier.issn1671-4083-
dc.identifier.urihttp://hdl.handle.net/10722/195719-
dc.description.abstractAIM: The purpose of the present study was to investigate the anticancer activity of bornyl caffeate in the human breast cancer cell line MCF-7. METHODS: The cell viability was determined using the MTT assay, and apoptosis was initially defined by monitoring the morphology of the cell nuclei and staining an early apoptotic biomarker with Annexin V-FITC. The mitochondrial membrane potential was visualized by JC-1 under fluorescence microscopy, whereas intracellular reactive oxygen species (ROS) were assessed by flow cytometry. The expression of apoptosis-associated proteins was determined by Western blotting analysis. RESULTS: Bornyl caffeate induced apoptosis in MCF-7 cells in a dose- and time-dependent manner. Consistently, bornyl caffeate increased Bax and decreased Bcl-xl, resulting in the disruption of MMP and subsequent activation of caspase-3. Moreover, bornyl caffeate triggered the formation of ROS and the activation of the mitogen-activated protein (MAP) kinases p38 and c-Jun N-terminal kinase (JNK). Antioxidants attenuated the activation of MAP kinase p38 but barely affected the activation of JNK. Importantly, the cytotoxicity of bornyl caffeate was partially attenuated by scavenging ROS and inhibited by MAP kinases and caspases. CONCLUSION: The present study demonstrated that bornyl caffeate induced apoptosis in the cancer cell line MCF-7 via activating the ROS- and JNK-mediated pathways. Thus, bornyl caffeate may be a potential anticancer lead compound.en_US
dc.languageengen_US
dc.publisherNature Publishing Group. The Journal's web site is located at http://www.nature.com/aps/index.html-
dc.relation.ispartofActa Pharmacologica Sinicaen_US
dc.titleBornyl caffeate induces apoptosis in human breast cancer MCF-7 cells via the ROS- and JNK-mediated pathwaysen_US
dc.typeArticleen_US
dc.identifier.emailRong, J: jrong@hku.hken_US
dc.identifier.authorityRong, J=rp00515en_US
dc.identifier.doi10.1038/aps.2013.162-
dc.identifier.pmid24335836-
dc.identifier.hkuros228240en_US
dc.identifier.volume35en_US
dc.identifier.issue1en_US
dc.identifier.spage113en_US
dc.identifier.epage123en_US
dc.identifier.isiWOS:000330581000013-
dc.publisher.placeUnited States-

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