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Article: Shiga toxin 2 targets the murine renal collecting duct epithelium

TitleShiga toxin 2 targets the murine renal collecting duct epithelium
Authors
Issue Date2009
Citation
Infection and Immunity, 2009, v. 77 n. 3, p. 959-969 How to Cite?
AbstractHemolytic-uremic syndrome (HUS) caused by Shiga toxin-producing Escherichia coli infection is a leading cause of pediatric acute renal failure. Bacterial toxins produced in the gut enter the circulation and cause a systemic toxemia and targeted cell damage. It had been previously shown that injection of Shiga toxin 2 (Stx2) and lipopolysaccharide (LPS) caused signs and symptoms of HUS in mice, but the mechanism leading to renal failure remained uncharacterized. The current study elucidated that murine cells of the glomerular filtration barrier were unresponsive to Stx2 because they lacked the receptor glycosphingolipid globotriaosylceramide (Gb 3) in vitro and in vivo. In contrast to the analogous human cells, Stx2 did not alter inflammatory kinase activity, cytokine release, or cell viability of the murine glomerular cells. However, murine renal cortical and medullary tubular cells expressed Gb 3 and responded to Stx2 by undergoing apoptosis. Stx2-induced loss of functioning collecting ducts in vivo caused production of increased dilute urine, resulted in dehydration, and contributed to renal failure. Stx2-mediated renal dysfunction was ameliorated by administration of the nonselective caspase inhibitor Q-VD-OPH in vivo. Stx2 therefore targets the murine collecting duct, and this Stx2-induced injury can be blocked by inhibitors of apoptosis in vivo. Copyright © 2009, American Society for Microbiology. All Rights Reserved.
Persistent Identifierhttp://hdl.handle.net/10722/195470
ISSN
2015 Impact Factor: 3.603
2015 SCImago Journal Rankings: 2.342
ISI Accession Number ID

 

DC FieldValueLanguage
dc.contributor.authorPsotka, MA-
dc.contributor.authorObata, F-
dc.contributor.authorRolling, GL-
dc.contributor.authorGross, LK-
dc.contributor.authorAjsaleem, M-
dc.contributor.authorSatchell, SC-
dc.contributor.authorMathieson, PW-
dc.contributor.authorObrig, TG-
dc.date.accessioned2014-02-28T06:12:12Z-
dc.date.available2014-02-28T06:12:12Z-
dc.date.issued2009-
dc.identifier.citationInfection and Immunity, 2009, v. 77 n. 3, p. 959-969-
dc.identifier.issn0019-9567-
dc.identifier.urihttp://hdl.handle.net/10722/195470-
dc.description.abstractHemolytic-uremic syndrome (HUS) caused by Shiga toxin-producing Escherichia coli infection is a leading cause of pediatric acute renal failure. Bacterial toxins produced in the gut enter the circulation and cause a systemic toxemia and targeted cell damage. It had been previously shown that injection of Shiga toxin 2 (Stx2) and lipopolysaccharide (LPS) caused signs and symptoms of HUS in mice, but the mechanism leading to renal failure remained uncharacterized. The current study elucidated that murine cells of the glomerular filtration barrier were unresponsive to Stx2 because they lacked the receptor glycosphingolipid globotriaosylceramide (Gb 3) in vitro and in vivo. In contrast to the analogous human cells, Stx2 did not alter inflammatory kinase activity, cytokine release, or cell viability of the murine glomerular cells. However, murine renal cortical and medullary tubular cells expressed Gb 3 and responded to Stx2 by undergoing apoptosis. Stx2-induced loss of functioning collecting ducts in vivo caused production of increased dilute urine, resulted in dehydration, and contributed to renal failure. Stx2-mediated renal dysfunction was ameliorated by administration of the nonselective caspase inhibitor Q-VD-OPH in vivo. Stx2 therefore targets the murine collecting duct, and this Stx2-induced injury can be blocked by inhibitors of apoptosis in vivo. Copyright © 2009, American Society for Microbiology. All Rights Reserved.-
dc.languageeng-
dc.relation.ispartofInfection and Immunity-
dc.titleShiga toxin 2 targets the murine renal collecting duct epithelium-
dc.typeArticle-
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1128/IAI.00679-08-
dc.identifier.pmid19124603-
dc.identifier.scopuseid_2-s2.0-62449248428-
dc.identifier.volume77-
dc.identifier.issue3-
dc.identifier.spage959-
dc.identifier.epage969-
dc.identifier.isiWOS:000263416700005-

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