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Article: The molecular and functional phenotype of glomerular podocytes reveals key features of contractile smooth muscle cells

TitleThe molecular and functional phenotype of glomerular podocytes reveals key features of contractile smooth muscle cells
Authors
Issue Date2008
Citation
American Journal of Physiology - Renal Physiology, 2008, v. 295 n. 4, p. F959-F970 How to Cite?
AbstractThe glomerular podocyte is a highly specialized cell, with the ability to ultrafilter blood and support glomerular capillary pressures. However, little is known about either the genetic programs leading to this functionality or the final phenotype. We approached this question utilizing a human conditionally immortalized cell line, which differentiates from a proliferating epithelial phenotype to a differentiated form. We profiled gene expression during several time points during differentiation and grouped the regulated genes into major functional categories. A novel category of genes that was upregulated during differentiation was of smooth muscle-related molecules. We further examined the smooth muscle phenotype and showed that podocytes consistently express the differentiated smooth muscle markers smoothelin and calponin and the specific transcription factor myocardin, both in vitro and in vivo. The contractile contribution of the podocyte to the glomerular capillary is controversial. We demonstrated using two novel techniques that podocytes contract vigorously in vitro when differentiated and in real time were able to demonstrate that angiotensin II treatment decreases monolayer resistance, morphologically correlating with enhanced contractility. We conclude that the mature podocyte in vitro possesses functional apparatus of contractile smooth muscle cells, with potential implications for its in vivo ability to regulate glomerular dynamic and permeability characteristics. Copyright © 2008 the American Physiological Society.
Persistent Identifierhttp://hdl.handle.net/10722/195467
ISSN
2008 Impact Factor: 3.89
ISI Accession Number ID

 

DC FieldValueLanguage
dc.contributor.authorSaleem, MA-
dc.contributor.authorZavadil, J-
dc.contributor.authorBailly, M-
dc.contributor.authorMcGee, K-
dc.contributor.authorWitherden, IR-
dc.contributor.authorPavenstadt, H-
dc.contributor.authorHsu, H-
dc.contributor.authorSanday, J-
dc.contributor.authorSatchell, SC-
dc.contributor.authorLennon, R-
dc.contributor.authorNi, L-
dc.contributor.authorBottinger, EP-
dc.contributor.authorMundel, P-
dc.contributor.authorMathieson, PW-
dc.date.accessioned2014-02-28T06:12:12Z-
dc.date.available2014-02-28T06:12:12Z-
dc.date.issued2008-
dc.identifier.citationAmerican Journal of Physiology - Renal Physiology, 2008, v. 295 n. 4, p. F959-F970-
dc.identifier.issn0363-6127-
dc.identifier.urihttp://hdl.handle.net/10722/195467-
dc.description.abstractThe glomerular podocyte is a highly specialized cell, with the ability to ultrafilter blood and support glomerular capillary pressures. However, little is known about either the genetic programs leading to this functionality or the final phenotype. We approached this question utilizing a human conditionally immortalized cell line, which differentiates from a proliferating epithelial phenotype to a differentiated form. We profiled gene expression during several time points during differentiation and grouped the regulated genes into major functional categories. A novel category of genes that was upregulated during differentiation was of smooth muscle-related molecules. We further examined the smooth muscle phenotype and showed that podocytes consistently express the differentiated smooth muscle markers smoothelin and calponin and the specific transcription factor myocardin, both in vitro and in vivo. The contractile contribution of the podocyte to the glomerular capillary is controversial. We demonstrated using two novel techniques that podocytes contract vigorously in vitro when differentiated and in real time were able to demonstrate that angiotensin II treatment decreases monolayer resistance, morphologically correlating with enhanced contractility. We conclude that the mature podocyte in vitro possesses functional apparatus of contractile smooth muscle cells, with potential implications for its in vivo ability to regulate glomerular dynamic and permeability characteristics. Copyright © 2008 the American Physiological Society.-
dc.languageeng-
dc.relation.ispartofAmerican Journal of Physiology - Renal Physiology-
dc.titleThe molecular and functional phenotype of glomerular podocytes reveals key features of contractile smooth muscle cells-
dc.typeArticle-
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1152/ajprenal.00559.2007-
dc.identifier.pmid18684887-
dc.identifier.scopuseid_2-s2.0-57049126587-
dc.identifier.volume295-
dc.identifier.issue4-
dc.identifier.spageF959-
dc.identifier.epageF970-
dc.identifier.isiWOS:000259913000013-

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