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Article: Cloning of a partial cDNA for rat interleukin-12 (IL-12) and analysis of IL-12 expression in vivo

TitleCloning of a partial cDNA for rat interleukin-12 (IL-12) and analysis of IL-12 expression in vivo
Authors
Issue Date1996
Citation
Scandinavian Journal of Immunology, 1996, v. 44 n. 1, p. 11-14 How to Cite?
AbstractExperimental models of autoimmunity in the rat may feature selective activation of either the Th1 or Th2 subset of helper T cells. Interleukin-12 (IL-12) is a key cytokine in the development of Th1 responses. In order to study IL-12 in the rat we used polymerase chain reaction (PCR) primers based on murine IL-12 to amplify a partial cDNA from rat tissue. The product was cloned and sequenced: it shows 94% nucleotide identity with the murine gene and 94% identity of predicted amino acid sequence. Primers based on the rat IL-12 sequence were used to analyse IL-12 expression in vivo using semi-quantitative PCR. We studied RNA from lymphoid tissues of two rat strains which differ in their response to mercuric chloride (HgCl2): Brown Norway (BN) rats develop autoimmunity with a predominant Th2 response; Lewis rats are resistant. Interleukin-12 expression was higher in Lewis than BN, and higher in spleen than lymph node. After HgCl2, IL-12 expression increased in BN towards the time when the autoimmune response autoregulates. Variation in baseline levels of IL-12 expression may account for the Th2 predisposition of BN rats compared to Lewis rats; IL-12 may play a role in the autoregulation of the Th2 response induced by HgCl2.
Persistent Identifierhttp://hdl.handle.net/10722/195343
ISSN
2015 Impact Factor: 2.27
2015 SCImago Journal Rankings: 0.934
ISI Accession Number ID

 

DC FieldValueLanguage
dc.contributor.authorMathieson, PW-
dc.contributor.authorGillespie, KM-
dc.date.accessioned2014-02-28T06:12:00Z-
dc.date.available2014-02-28T06:12:00Z-
dc.date.issued1996-
dc.identifier.citationScandinavian Journal of Immunology, 1996, v. 44 n. 1, p. 11-14-
dc.identifier.issn0300-9475-
dc.identifier.urihttp://hdl.handle.net/10722/195343-
dc.description.abstractExperimental models of autoimmunity in the rat may feature selective activation of either the Th1 or Th2 subset of helper T cells. Interleukin-12 (IL-12) is a key cytokine in the development of Th1 responses. In order to study IL-12 in the rat we used polymerase chain reaction (PCR) primers based on murine IL-12 to amplify a partial cDNA from rat tissue. The product was cloned and sequenced: it shows 94% nucleotide identity with the murine gene and 94% identity of predicted amino acid sequence. Primers based on the rat IL-12 sequence were used to analyse IL-12 expression in vivo using semi-quantitative PCR. We studied RNA from lymphoid tissues of two rat strains which differ in their response to mercuric chloride (HgCl2): Brown Norway (BN) rats develop autoimmunity with a predominant Th2 response; Lewis rats are resistant. Interleukin-12 expression was higher in Lewis than BN, and higher in spleen than lymph node. After HgCl2, IL-12 expression increased in BN towards the time when the autoimmune response autoregulates. Variation in baseline levels of IL-12 expression may account for the Th2 predisposition of BN rats compared to Lewis rats; IL-12 may play a role in the autoregulation of the Th2 response induced by HgCl2.-
dc.languageeng-
dc.relation.ispartofScandinavian Journal of Immunology-
dc.titleCloning of a partial cDNA for rat interleukin-12 (IL-12) and analysis of IL-12 expression in vivo-
dc.typeArticle-
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1046/j.1365-3083.1996.d01-279.x-
dc.identifier.pmid8693286-
dc.identifier.scopuseid_2-s2.0-0029973491-
dc.identifier.volume44-
dc.identifier.issue1-
dc.identifier.spage11-
dc.identifier.epage14-
dc.identifier.isiWOS:A1996UW04100002-

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