File Download

There are no files associated with this item.

  Links for fulltext
     (May Require Subscription)
Supplementary

Article: Th1/Th2 cytokine gene expression after mercuric chloride in susceptible and resistant rat strains

TitleTh1/Th2 cytokine gene expression after mercuric chloride in susceptible and resistant rat strains
Authors
Issue Date1996
Citation
European Journal of Immunology, 1996, v. 26 n. 10, p. 2388-2392 How to Cite?
AbstractMercuric chloride (HgCl 2) has contrasting effects on different rat strains: susceptible strains, e.g. Brown Norway (BN) develop polyclonal B cell activation, multiple autoantibodies and widespread tissue injury. Lewis (LEW) rats are resistant: no autoimmune response occurs after HgCl 2; instead, there is immunosuppression. We have previously shown, by fully quantitative polymerase chain reaction (PCR), up-regulation of interleukin-4, (IL-4) gene expression in HgCl 2-treated BN rats, implicating Th2 cells in the autoimmune syndrome. Involvement of the reciprocal Th1 subset, producing interferon-γ (IFN-γ), in resistance of LEW rats to HgCl 2 has been suggested. We now report extensive analysis of Th1 and Th2 cytokine gene expression in spleen and lymph nodes of susceptible (BN) and resistant (LEW) rats after HgCl 2. IL-4 and TFN-γ were analyzed by quantitative PCR, other cytokines were assessed using semiquantitative PCR: the relative merits of these two techniques are discussed. We show pronounced up-regulation of IL-4 and more modest up-regulation of IFN-γ in BN rats, but no up-regulation of either in LEW rats. Baseline levels of IFN-γ were higher in LEW rats. Semi-quantitative PCR showed increased expression of IL-2, IL-6 and IL-10 in BN; in LEW rats only IL-10 was increased. There was no marked change in IL-5, IL-13 or transforming growth factor-β (TGF-β) in either strain. Those data further support the key role of IL-4 in HgCl 2-induced autoimmunity, and suggest that failure of up-regulation of IL-4, together with higher baseline IFN-γ expression, accounts for resistance of LEW rats to HgCl 2. However, neither IFN-γ nor TGF-β can be implicated in HgCl 2-induced immunosuppression in the LEW rat in vivo: our data suggest a role for IL-10 in this phenomenon.
Persistent Identifierhttp://hdl.handle.net/10722/195340
ISSN
2015 Impact Factor: 4.179
2015 SCImago Journal Rankings: 2.568
ISI Accession Number ID

 

DC FieldValueLanguage
dc.contributor.authorGillespie, KM-
dc.contributor.authorSaoudi, A-
dc.contributor.authorKuhn, J-
dc.contributor.authorWhittle, CJ-
dc.contributor.authorDruet, P-
dc.contributor.authorBellon, B-
dc.contributor.authorMathieson, PW-
dc.date.accessioned2014-02-28T06:12:00Z-
dc.date.available2014-02-28T06:12:00Z-
dc.date.issued1996-
dc.identifier.citationEuropean Journal of Immunology, 1996, v. 26 n. 10, p. 2388-2392-
dc.identifier.issn0014-2980-
dc.identifier.urihttp://hdl.handle.net/10722/195340-
dc.description.abstractMercuric chloride (HgCl 2) has contrasting effects on different rat strains: susceptible strains, e.g. Brown Norway (BN) develop polyclonal B cell activation, multiple autoantibodies and widespread tissue injury. Lewis (LEW) rats are resistant: no autoimmune response occurs after HgCl 2; instead, there is immunosuppression. We have previously shown, by fully quantitative polymerase chain reaction (PCR), up-regulation of interleukin-4, (IL-4) gene expression in HgCl 2-treated BN rats, implicating Th2 cells in the autoimmune syndrome. Involvement of the reciprocal Th1 subset, producing interferon-γ (IFN-γ), in resistance of LEW rats to HgCl 2 has been suggested. We now report extensive analysis of Th1 and Th2 cytokine gene expression in spleen and lymph nodes of susceptible (BN) and resistant (LEW) rats after HgCl 2. IL-4 and TFN-γ were analyzed by quantitative PCR, other cytokines were assessed using semiquantitative PCR: the relative merits of these two techniques are discussed. We show pronounced up-regulation of IL-4 and more modest up-regulation of IFN-γ in BN rats, but no up-regulation of either in LEW rats. Baseline levels of IFN-γ were higher in LEW rats. Semi-quantitative PCR showed increased expression of IL-2, IL-6 and IL-10 in BN; in LEW rats only IL-10 was increased. There was no marked change in IL-5, IL-13 or transforming growth factor-β (TGF-β) in either strain. Those data further support the key role of IL-4 in HgCl 2-induced autoimmunity, and suggest that failure of up-regulation of IL-4, together with higher baseline IFN-γ expression, accounts for resistance of LEW rats to HgCl 2. However, neither IFN-γ nor TGF-β can be implicated in HgCl 2-induced immunosuppression in the LEW rat in vivo: our data suggest a role for IL-10 in this phenomenon.-
dc.languageeng-
dc.relation.ispartofEuropean Journal of Immunology-
dc.titleTh1/Th2 cytokine gene expression after mercuric chloride in susceptible and resistant rat strains-
dc.typeArticle-
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1002/eji.1830261018-
dc.identifier.pmid8898950-
dc.identifier.scopuseid_2-s2.0-0029801050-
dc.identifier.volume26-
dc.identifier.issue10-
dc.identifier.spage2388-
dc.identifier.epage2392-
dc.identifier.isiWOS:A1996VM20000017-

Export via OAI-PMH Interface in XML Formats


OR


Export to Other Non-XML Formats