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Article: Isoprenaline stimulates gene transcription of the inhibitory G protein α-subunit Giα-2 in rat heart

TitleIsoprenaline stimulates gene transcription of the inhibitory G protein α-subunit Giα-2 in rat heart
Authors
Issue Date1993
Citation
Circulation Research, 1993, v. 72 n. 3, p. 696-700 How to Cite?
AbstractIn vitro transcription reactions were performed with isolated ventricular nuclei of adult rats to investigate whether increased mRNA levels of the inhibitory G protein α-subunit Giα-2 after prolonged in vivo stimulation with the β-adrenoceptor agonist isoprenaline are caused by increased transcription. Rats were treated by a 4-day subcutaneous infusion of isoprenaline (2.4 mg/kg per day) or 0.9% NaCl as control. To avoid the influence of developmental expression patterns, adult rats were chosen for all experiments. Signals for Giα-2 and the stimulatory G protein α-subunit Gsα were specific and due to hybridization of nascent mRNA transcripts. In the isoprenaline group the transcriptional activity of Giα-2 gene increased to 140% of the control value, whereas gene specific hybridization for Gsα remained unchanged. These results show that increased Giα-2 mRNA levels after stimulation with isoprenaline are at least partially caused by enhanced transcription of Giα-2 mRNA.
Persistent Identifierhttp://hdl.handle.net/10722/195234
ISSN
2015 Impact Factor: 11.551
2015 SCImago Journal Rankings: 5.755
ISI Accession Number ID

 

DC FieldValueLanguage
dc.contributor.authorMüller, FU-
dc.contributor.authorBoheler, KR-
dc.contributor.authorEschenhagen, T-
dc.contributor.authorSchmitz, W-
dc.contributor.authorScholz, H-
dc.date.accessioned2014-02-25T01:40:20Z-
dc.date.available2014-02-25T01:40:20Z-
dc.date.issued1993-
dc.identifier.citationCirculation Research, 1993, v. 72 n. 3, p. 696-700-
dc.identifier.issn0009-7330-
dc.identifier.urihttp://hdl.handle.net/10722/195234-
dc.description.abstractIn vitro transcription reactions were performed with isolated ventricular nuclei of adult rats to investigate whether increased mRNA levels of the inhibitory G protein α-subunit Giα-2 after prolonged in vivo stimulation with the β-adrenoceptor agonist isoprenaline are caused by increased transcription. Rats were treated by a 4-day subcutaneous infusion of isoprenaline (2.4 mg/kg per day) or 0.9% NaCl as control. To avoid the influence of developmental expression patterns, adult rats were chosen for all experiments. Signals for Giα-2 and the stimulatory G protein α-subunit Gsα were specific and due to hybridization of nascent mRNA transcripts. In the isoprenaline group the transcriptional activity of Giα-2 gene increased to 140% of the control value, whereas gene specific hybridization for Gsα remained unchanged. These results show that increased Giα-2 mRNA levels after stimulation with isoprenaline are at least partially caused by enhanced transcription of Giα-2 mRNA.-
dc.languageeng-
dc.relation.ispartofCirculation Research-
dc.titleIsoprenaline stimulates gene transcription of the inhibitory G protein α-subunit Giα-2 in rat heart-
dc.typeArticle-
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.pmid8431992-
dc.identifier.scopuseid_2-s2.0-0027419942-
dc.identifier.volume72-
dc.identifier.issue3-
dc.identifier.spage696-
dc.identifier.epage700-
dc.identifier.isiWOS:A1993KM46300021-

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