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Article: Diabetes decreases creatine kinase enzyme activity and mRNA level in the rat heart

TitleDiabetes decreases creatine kinase enzyme activity and mRNA level in the rat heart
Authors
Issue Date1989
Citation
American Journal of Physiology - Endocrinology and Metabolism, 1989, v. 257 n. 4, p. 20/4 How to Cite?
AbstractSeveral of the adenosinetriphosphatase enzymes that are responsible for cardiac muscle contraction rely on high-energy phosphates supplied by the creatine kinase (CK) system. Experimental diabetes mellitus has been shown to cause a decrease in the maximal contractile performance of the heart. We postulated that the decrease in contractile performance may be explained in part by a decrease in CK enzyme activity. To evaluate this possibility, we determined the level of CK activity and isoenzyme distribution in ventricular homogenates from normal, diabetic, and insulin-treated diabetic rats. We found that total CK activity was decreased by 35% in diabetic hearts and that a 66% reduction in the cardiac-specific MB isoenzyme occurs. Using a cDNA probe for CK-muscle (M) RNA in Northern blot analysis, we determined that a 61.1% decrease in CK-M mRNA occurs in diabetes. Chronic insulin therapy for 1 mo restores CK-M mRNA levels and enzyme activity. In conclusion, diabetes-induced CK enzyme decreases are mediated in part by a lower level of CK-M mRNA that codes for the major CK-M subunit protein. Decreased performance of the CK system may contribute to diabetic cardiomyopathy.
Persistent Identifierhttp://hdl.handle.net/10722/195219
ISSN
1998 Impact Factor: 3.077
2004 SCImago Journal Rankings: 1.102
ISI Accession Number ID

 

DC FieldValueLanguage
dc.contributor.authorPopovich, BK-
dc.contributor.authorBoheler, KR-
dc.contributor.authorDillmann, WH-
dc.date.accessioned2014-02-25T01:40:19Z-
dc.date.available2014-02-25T01:40:19Z-
dc.date.issued1989-
dc.identifier.citationAmerican Journal of Physiology - Endocrinology and Metabolism, 1989, v. 257 n. 4, p. 20/4-
dc.identifier.issn0002-9513-
dc.identifier.urihttp://hdl.handle.net/10722/195219-
dc.description.abstractSeveral of the adenosinetriphosphatase enzymes that are responsible for cardiac muscle contraction rely on high-energy phosphates supplied by the creatine kinase (CK) system. Experimental diabetes mellitus has been shown to cause a decrease in the maximal contractile performance of the heart. We postulated that the decrease in contractile performance may be explained in part by a decrease in CK enzyme activity. To evaluate this possibility, we determined the level of CK activity and isoenzyme distribution in ventricular homogenates from normal, diabetic, and insulin-treated diabetic rats. We found that total CK activity was decreased by 35% in diabetic hearts and that a 66% reduction in the cardiac-specific MB isoenzyme occurs. Using a cDNA probe for CK-muscle (M) RNA in Northern blot analysis, we determined that a 61.1% decrease in CK-M mRNA occurs in diabetes. Chronic insulin therapy for 1 mo restores CK-M mRNA levels and enzyme activity. In conclusion, diabetes-induced CK enzyme decreases are mediated in part by a lower level of CK-M mRNA that codes for the major CK-M subunit protein. Decreased performance of the CK system may contribute to diabetic cardiomyopathy.-
dc.languageeng-
dc.relation.ispartofAmerican Journal of Physiology - Endocrinology and Metabolism-
dc.titleDiabetes decreases creatine kinase enzyme activity and mRNA level in the rat heart-
dc.typeArticle-
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.pmid2679131-
dc.identifier.scopuseid_2-s2.0-0024432530-
dc.identifier.volume257-
dc.identifier.issue4-
dc.identifier.spage20/4-
dc.identifier.epage20/4-
dc.identifier.isiWOS:A1989CC64500048-

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