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Article: Cardiac response to pressure overload in the rat: The selective alteration of in vitro directed RNA translation products

TitleCardiac response to pressure overload in the rat: The selective alteration of in vitro directed RNA translation products
Authors
Issue Date1988
Citation
Circulation Research, 1988, v. 63 n. 2, p. 448-456 How to Cite?
AbstractAs cardiac hypertrophy develops, total cardiac RNA and protein synthesis increase significantly. We have identified specific messenger RNAs that change in predominance with the induction of pressure-overload-stimulated cardiac hypertrophy. Total cardiac RNA was isolated from rats either undergoing cardiac hypertrophy secondary to subdiaphragmatic aortic constriction or subjected to sham surgery. The products translated in vitro were separated by two-dimensional gel electrophoresis and quantitated. The translation of four proteins decreased while the translation of four others increased in preparations from hypertrophied hearts compared with those from sham-treated rats. Two isoforms of creatine kinase M were translated in vitro. Only one of these isoforms decreased with cardiac hypertrophy, suggesting that the transcriptional or translation control for creatine kinase is much more complex than previously believed. Finally, since only eight of over 700 different translation products change in relative predominance with cardiac hypertrophy, we conlude that the accumulation of existing RNA and protein products is the primary adaptive process responsible for cardiac hypertrophy.
Persistent Identifierhttp://hdl.handle.net/10722/195216
ISSN
2015 Impact Factor: 11.551
2015 SCImago Journal Rankings: 5.755
ISI Accession Number ID

 

DC FieldValueLanguage
dc.contributor.authorBoheler, KR-
dc.contributor.authorDillmann, WH-
dc.date.accessioned2014-02-25T01:40:19Z-
dc.date.available2014-02-25T01:40:19Z-
dc.date.issued1988-
dc.identifier.citationCirculation Research, 1988, v. 63 n. 2, p. 448-456-
dc.identifier.issn0009-7330-
dc.identifier.urihttp://hdl.handle.net/10722/195216-
dc.description.abstractAs cardiac hypertrophy develops, total cardiac RNA and protein synthesis increase significantly. We have identified specific messenger RNAs that change in predominance with the induction of pressure-overload-stimulated cardiac hypertrophy. Total cardiac RNA was isolated from rats either undergoing cardiac hypertrophy secondary to subdiaphragmatic aortic constriction or subjected to sham surgery. The products translated in vitro were separated by two-dimensional gel electrophoresis and quantitated. The translation of four proteins decreased while the translation of four others increased in preparations from hypertrophied hearts compared with those from sham-treated rats. Two isoforms of creatine kinase M were translated in vitro. Only one of these isoforms decreased with cardiac hypertrophy, suggesting that the transcriptional or translation control for creatine kinase is much more complex than previously believed. Finally, since only eight of over 700 different translation products change in relative predominance with cardiac hypertrophy, we conlude that the accumulation of existing RNA and protein products is the primary adaptive process responsible for cardiac hypertrophy.-
dc.languageeng-
dc.relation.ispartofCirculation Research-
dc.titleCardiac response to pressure overload in the rat: The selective alteration of in vitro directed RNA translation products-
dc.typeArticle-
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.pmid2456165-
dc.identifier.scopuseid_2-s2.0-0023682695-
dc.identifier.volume63-
dc.identifier.issue2-
dc.identifier.spage448-
dc.identifier.epage456-
dc.identifier.isiWOS:A1988P634600019-

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