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Article: Proliferation of mouse embryonic stem cell progeny and the spontaneous contractile activity of cardiomyocytes are affected by microtopography

TitleProliferation of mouse embryonic stem cell progeny and the spontaneous contractile activity of cardiomyocytes are affected by microtopography
Authors
Issue Date2008
Citation
Developmental Dynamics, 2008, v. 238 n. 8, p. 1964-1973 How to Cite?
AbstractThe niche in which stem cells reside and differentiate is a complex physicochemical microenvironment that regulates cell function. The role played by three-dimensional physical contours was studied on cell progeny derived from mouse embryonic stem cells using microtopographies created on PDMS (poly-dimethylsiloxane) membranes. While markers of differentiation were not affected, the proliferation of heterogeneous mouse embryonic stem cell-derived progeny was attenuated by 15 μm-, but not 5 μm-high microprojections. This reduction was reversed by Rho kinase and myosin light chain kinase inhibition, which diminishes the tension generating ability of stress fibers. Purified cardiomyocytes derived from embryonic stem cells also showed significant blunting of proliferation and increased beating rates compared with cells grown on flat substrates. Thus, proliferation of stem cell-derived progeny appears to be regulated by microtopography through tension-generation of contractility in the third-dimension. These results emphasize the importance of topographic cues in the modulation of stem cell progeny behavior. © 2009 Wiley-Liss, Inc.
Persistent Identifierhttp://hdl.handle.net/10722/195199
ISSN
2015 Impact Factor: 2.198
2015 SCImago Journal Rankings: 1.726
ISI Accession Number ID

 

DC FieldValueLanguage
dc.contributor.authorBiehl, JK-
dc.contributor.authorYamanaka, S-
dc.contributor.authorDesai, TA-
dc.contributor.authorBoheler, KR-
dc.contributor.authorRussell, B-
dc.date.accessioned2014-02-25T01:40:18Z-
dc.date.available2014-02-25T01:40:18Z-
dc.date.issued2008-
dc.identifier.citationDevelopmental Dynamics, 2008, v. 238 n. 8, p. 1964-1973-
dc.identifier.issn1058-8388-
dc.identifier.urihttp://hdl.handle.net/10722/195199-
dc.description.abstractThe niche in which stem cells reside and differentiate is a complex physicochemical microenvironment that regulates cell function. The role played by three-dimensional physical contours was studied on cell progeny derived from mouse embryonic stem cells using microtopographies created on PDMS (poly-dimethylsiloxane) membranes. While markers of differentiation were not affected, the proliferation of heterogeneous mouse embryonic stem cell-derived progeny was attenuated by 15 μm-, but not 5 μm-high microprojections. This reduction was reversed by Rho kinase and myosin light chain kinase inhibition, which diminishes the tension generating ability of stress fibers. Purified cardiomyocytes derived from embryonic stem cells also showed significant blunting of proliferation and increased beating rates compared with cells grown on flat substrates. Thus, proliferation of stem cell-derived progeny appears to be regulated by microtopography through tension-generation of contractility in the third-dimension. These results emphasize the importance of topographic cues in the modulation of stem cell progeny behavior. © 2009 Wiley-Liss, Inc.-
dc.languageeng-
dc.relation.ispartofDevelopmental Dynamics-
dc.titleProliferation of mouse embryonic stem cell progeny and the spontaneous contractile activity of cardiomyocytes are affected by microtopography-
dc.typeArticle-
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1002/dvdy.22030-
dc.identifier.pmid19618471-
dc.identifier.scopuseid_2-s2.0-67650815287-
dc.identifier.volume238-
dc.identifier.issue8-
dc.identifier.spage1964-
dc.identifier.epage1973-
dc.identifier.isiWOS:000268685100009-

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