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Article: Sex- and age-dependent human transcriptome variability: Implications for chronic heart failure

TitleSex- and age-dependent human transcriptome variability: Implications for chronic heart failure
Authors
Issue Date2003
Citation
Proceedings of the National Academy of Sciences of the United States of America, 2003, v. 100 n. 5, p. 2754-2759 How to Cite?
AbstractHeart failure (HF) is the end result of progressive and diverse biological adaptations within the diseased myocardium. We used cDNA microarrays and quantitative PCR to examine the transcriptomes of 38 left ventricles from failing and nonfailing human myocardium. After identification of a pool of putative HF-responsive candidate genes by microarrays on seven nonfailing and eight failing hearts, we used quantitative PCR and a general linear statistical model in a larger sample set (n = 34) to validate and examine the role of contributing biological variables (age and sex). We find that most HF-candidate genes (transcription factors, Cebpb, Npat; signaling molecules, Map2k3, Map4k5; extracellular matrix proteins, Lum, Cola1; and metabolic enzymes, Mars) demonstrated significant changes in gene expression; however, the majority of differences among samples depended on variables such as sex and age, and not on HF alone. Some HF-responsive gene products also demonstrated highly significant changes in expression as a function of age and/or sex, but independent of HF (Ngp1, Cd163, and Npat). These results emphasize the need to account for biological variables (HF, sex and age interactions) to elucidate genomic correlates that trigger molecular pathways responsible for the progression of HF syndromes.
Persistent Identifierhttp://hdl.handle.net/10722/195166
ISSN
2015 Impact Factor: 9.423
2015 SCImago Journal Rankings: 6.883
ISI Accession Number ID

 

DC FieldValueLanguage
dc.contributor.authorBoheler, KR-
dc.contributor.authorVolkova, M-
dc.contributor.authorMorrell, C-
dc.contributor.authorGarg, R-
dc.contributor.authorZhu, Y-
dc.contributor.authorMargulies, K-
dc.contributor.authorSeymour, A-M-
dc.contributor.authorLakatta, EG-
dc.date.accessioned2014-02-25T01:40:15Z-
dc.date.available2014-02-25T01:40:15Z-
dc.date.issued2003-
dc.identifier.citationProceedings of the National Academy of Sciences of the United States of America, 2003, v. 100 n. 5, p. 2754-2759-
dc.identifier.issn0027-8424-
dc.identifier.urihttp://hdl.handle.net/10722/195166-
dc.description.abstractHeart failure (HF) is the end result of progressive and diverse biological adaptations within the diseased myocardium. We used cDNA microarrays and quantitative PCR to examine the transcriptomes of 38 left ventricles from failing and nonfailing human myocardium. After identification of a pool of putative HF-responsive candidate genes by microarrays on seven nonfailing and eight failing hearts, we used quantitative PCR and a general linear statistical model in a larger sample set (n = 34) to validate and examine the role of contributing biological variables (age and sex). We find that most HF-candidate genes (transcription factors, Cebpb, Npat; signaling molecules, Map2k3, Map4k5; extracellular matrix proteins, Lum, Cola1; and metabolic enzymes, Mars) demonstrated significant changes in gene expression; however, the majority of differences among samples depended on variables such as sex and age, and not on HF alone. Some HF-responsive gene products also demonstrated highly significant changes in expression as a function of age and/or sex, but independent of HF (Ngp1, Cd163, and Npat). These results emphasize the need to account for biological variables (HF, sex and age interactions) to elucidate genomic correlates that trigger molecular pathways responsible for the progression of HF syndromes.-
dc.languageeng-
dc.relation.ispartofProceedings of the National Academy of Sciences of the United States of America-
dc.titleSex- and age-dependent human transcriptome variability: Implications for chronic heart failure-
dc.typeArticle-
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1073/pnas.0436564100-
dc.identifier.pmid12601168-
dc.identifier.scopuseid_2-s2.0-0037418307-
dc.identifier.volume100-
dc.identifier.issue5-
dc.identifier.spage2754-
dc.identifier.epage2759-
dc.identifier.isiWOS:000181365000105-

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