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Article: Embryonic stem cell-derived cardiomyocyte heterogeneity and the isolation of immature and committed cells for cardiac remodeling and regeneration

TitleEmbryonic stem cell-derived cardiomyocyte heterogeneity and the isolation of immature and committed cells for cardiac remodeling and regeneration
Authors
Issue Date2011
Citation
Stem Cells International, 2011 How to Cite?
AbstractPluripotent stem cells represent one promising source for cell replacement therapy in heart, but differentiating embryonic stem cell-derived cardiomyocytes (ESC-CMs) are highly heterogeneous and show a variety of maturation states. In this study, we employed an ESC clonal line that contains a cardiac-restricted ncx1 promoter-driven puromycin resistance cassette together with a mass culture system to isolate ESC-CMs that display traits characteristic of very immature CMs. The cells display properties of proliferation, CM-restricted markers, reduced mitochondrial mass, and hypoxia-resistance. Following transplantation into rodent hearts, bioluminescence imaging revealed that immature cells, but not more mature CMs, survived for at least one month following injection. These data and comparisons with more mature cells lead us to conclude that immature hypoxia resistant ESC-CMs can be isolated in mass in vitro and, following injection into heart, form grafts that may mediate long-term recovery of global and regional myocardial contractile function following infarction. © 2011 Kenneth R. Boheler et al.
Persistent Identifierhttp://hdl.handle.net/10722/195142
ISSN
2015 SCImago Journal Rankings: 1.173
ISI Accession Number ID

 

DC FieldValueLanguage
dc.contributor.authorBoheler, KR-
dc.contributor.authorJoodi, RN-
dc.contributor.authorQiao, H-
dc.contributor.authorJuhasz, O-
dc.contributor.authorUrick, AL-
dc.contributor.authorChuppa, SL-
dc.contributor.authorGundry, RL-
dc.contributor.authorWersto, RP-
dc.contributor.authorZhou, R-
dc.date.accessioned2014-02-25T01:40:13Z-
dc.date.available2014-02-25T01:40:13Z-
dc.date.issued2011-
dc.identifier.citationStem Cells International, 2011-
dc.identifier.issn1687-9678-
dc.identifier.urihttp://hdl.handle.net/10722/195142-
dc.description.abstractPluripotent stem cells represent one promising source for cell replacement therapy in heart, but differentiating embryonic stem cell-derived cardiomyocytes (ESC-CMs) are highly heterogeneous and show a variety of maturation states. In this study, we employed an ESC clonal line that contains a cardiac-restricted ncx1 promoter-driven puromycin resistance cassette together with a mass culture system to isolate ESC-CMs that display traits characteristic of very immature CMs. The cells display properties of proliferation, CM-restricted markers, reduced mitochondrial mass, and hypoxia-resistance. Following transplantation into rodent hearts, bioluminescence imaging revealed that immature cells, but not more mature CMs, survived for at least one month following injection. These data and comparisons with more mature cells lead us to conclude that immature hypoxia resistant ESC-CMs can be isolated in mass in vitro and, following injection into heart, form grafts that may mediate long-term recovery of global and regional myocardial contractile function following infarction. © 2011 Kenneth R. Boheler et al.-
dc.languageeng-
dc.relation.ispartofStem Cells International-
dc.titleEmbryonic stem cell-derived cardiomyocyte heterogeneity and the isolation of immature and committed cells for cardiac remodeling and regeneration-
dc.typeArticle-
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.4061/2011/214203-
dc.identifier.scopuseid_2-s2.0-81555195709-
dc.identifier.isiWOS:000208760300008-

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