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Conference Paper: A comparative study of cell-free Epstein-Barr virus DNA and microRNAs in nasopharyngeal carcinoma screening

TitleA comparative study of cell-free Epstein-Barr virus DNA and microRNAs in nasopharyngeal carcinoma screening
Authors
KeywordsMedical sciences
Experimental medicine, laboratory technique
Issue Date2010
PublisherInternational Institute of Anticancer Research. The Journal's web site is located at iiar@iiar-anticancer.org
Citation
The 4th International Congress of Molecular Medicine, Istanbul, Turkey, 27-30 June, 2011. In in vivo, 2011, v. 25 n. 3, p. 541, abstract no. 195 How to Cite?
AbstractBACKGROUND: Nasopharyngeal carcinoma (NPC) is a malignant squamous cell carcinoma in the head and neck region. Epstein-Barr virus (EBV) is associated with undifferentiated NPC. Cell-free EBV DNA is routinely used as a serological marker. Circulating nucleic acids were recently suggested as non-invasive diagnosis markers. This study aims to examine the use of microRNAs (miRNAs) as a supplement to existing screening methods. METHODS: The expression levels of hsa-miR-21, hsa-miR-1301, ebv-miRBART7 and ebv-miR-BART22 were investigated in ten paired tumor-normal tissues, plasmas of sixty other NPC patients and twenty-five cohorts. Plasmas from thirteen NPC patients were evaluated for both EBV and miRNAs levels between pre-operative and post-operative (three months after radiotherapy) patients. MiRNA and EBV DNA quantitation were performed by quantitative real-time PCR and EBV quant kit, respectively. RESULTS: MiR-BART7 showed higher expression in tumor tissues (p=0.038) and in NPC plasma samples with sensitivity 71.15% and specificity 76.47%. Among patients who had already received radiotherapy, 12 of 13 patients showed elevated miR-BART7 expression whereas 4 of 13 patients showed elevated EBV DNA, 3 of 13 patients had increased and 5 of 13 undetermined. CONCLUSION: MiR-BART7 showed significantly higher expression in NPC with good sensitivity and specificity. Further studies will be undertaken on the functional role of miR-BART7.
Persistent Identifierhttp://hdl.handle.net/10722/194800
ISSN
2015 Impact Factor: 0.832
2015 SCImago Journal Rankings: 0.382

 

DC FieldValueLanguage
dc.contributor.authorMan, OYen_US
dc.contributor.authorWong, STSen_US
dc.contributor.authorChan, YWen_US
dc.contributor.authorWei, WIen_US
dc.date.accessioned2014-02-17T02:10:25Z-
dc.date.available2014-02-17T02:10:25Z-
dc.date.issued2010en_US
dc.identifier.citationThe 4th International Congress of Molecular Medicine, Istanbul, Turkey, 27-30 June, 2011. In in vivo, 2011, v. 25 n. 3, p. 541, abstract no. 195en_US
dc.identifier.issn0258-851X-
dc.identifier.urihttp://hdl.handle.net/10722/194800-
dc.description.abstractBACKGROUND: Nasopharyngeal carcinoma (NPC) is a malignant squamous cell carcinoma in the head and neck region. Epstein-Barr virus (EBV) is associated with undifferentiated NPC. Cell-free EBV DNA is routinely used as a serological marker. Circulating nucleic acids were recently suggested as non-invasive diagnosis markers. This study aims to examine the use of microRNAs (miRNAs) as a supplement to existing screening methods. METHODS: The expression levels of hsa-miR-21, hsa-miR-1301, ebv-miRBART7 and ebv-miR-BART22 were investigated in ten paired tumor-normal tissues, plasmas of sixty other NPC patients and twenty-five cohorts. Plasmas from thirteen NPC patients were evaluated for both EBV and miRNAs levels between pre-operative and post-operative (three months after radiotherapy) patients. MiRNA and EBV DNA quantitation were performed by quantitative real-time PCR and EBV quant kit, respectively. RESULTS: MiR-BART7 showed higher expression in tumor tissues (p=0.038) and in NPC plasma samples with sensitivity 71.15% and specificity 76.47%. Among patients who had already received radiotherapy, 12 of 13 patients showed elevated miR-BART7 expression whereas 4 of 13 patients showed elevated EBV DNA, 3 of 13 patients had increased and 5 of 13 undetermined. CONCLUSION: MiR-BART7 showed significantly higher expression in NPC with good sensitivity and specificity. Further studies will be undertaken on the functional role of miR-BART7.-
dc.languageengen_US
dc.publisherInternational Institute of Anticancer Research. The Journal's web site is located at iiar@iiar-anticancer.org-
dc.relation.ispartofin vivoen_US
dc.subjectMedical sciences-
dc.subjectExperimental medicine, laboratory technique-
dc.titleA comparative study of cell-free Epstein-Barr virus DNA and microRNAs in nasopharyngeal carcinoma screeningen_US
dc.typeConference_Paperen_US
dc.identifier.emailWong, STS: thiansze@graduate.hku.hken_US
dc.identifier.emailChan, YW: jywchan1@hku.hken_US
dc.identifier.emailWei, WI: hrmswwi@hku.hken_US
dc.identifier.authorityWong, STS=rp00478en_US
dc.identifier.authorityChan, YW=rp01314en_US
dc.description.naturelink_to_OA_fulltext-
dc.identifier.hkuros227809en_US
dc.identifier.volume25-
dc.identifier.issue3-
dc.identifier.spage541-
dc.identifier.epage541-
dc.publisher.placeGreece-

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