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Article: Specific down-regulation of HER-2/neu mediated by a chimeric U6 hammerhead ribozyme results in growth inhibition of human ovarian carcinoma

TitleSpecific down-regulation of HER-2/neu mediated by a chimeric U6 hammerhead ribozyme results in growth inhibition of human ovarian carcinoma
Authors
Issue Date2001
Citation
Molecular Therapy, 2001, v. 3 n. 2, p. 169-177 How to Cite?
AbstractThe U6 expression system was explored for efficient expression of a ribozyme against the human proto-oncogene c-neu. A hammerhead ribozyme (neuRz) and the control mutant ribozyme (MRz) were targeted to cleave c-neu mRNA at the tyrosine kinase domain. In vitro cleavage showed that neuRz was very active while MRz was not. Near-maximal target cleavage observed at a low ribozyme:target ratio (0.1) suggests that neuRz has good activity and turnover capability under physiological conditions, i.e., <5 mM MgCI2 and 37°C. Chimeric U6 ribozyme was expressed at about 5 × 106 copies/cell at 48 h in the ovarian carcinoma cell line SKOV-3.ip1. Partial down-regulation of c-neu mRNA and protein was observed in a dose-dependent manner in cells transiently transfected with U6neuRz- but not with MRz-containing plasmid. Sorted transient transfectants demonstrated dramatic growth inhibition with the neuRz-expressing cells. Our results demonstrate that the U6 expression system is very efficient and suitable for the expression of a hammerhead ribozyme. Moreover, nonviral delivery of the neuRz-expressing plasmid resulted in specific down-regulation of c-neu and, subsequently, growth inhibition of ovarian cancer cells overexpressing c-neu.
Persistent Identifierhttp://hdl.handle.net/10722/194120
ISSN
2015 Impact Factor: 6.938
2015 SCImago Journal Rankings: 3.374
ISI Accession Number ID

 

DC FieldValueLanguage
dc.contributor.authorLui, VWY-
dc.contributor.authorHe, Y-
dc.contributor.authorGoyaland, K-
dc.contributor.authorHuang, L-
dc.date.accessioned2014-01-30T03:32:11Z-
dc.date.available2014-01-30T03:32:11Z-
dc.date.issued2001-
dc.identifier.citationMolecular Therapy, 2001, v. 3 n. 2, p. 169-177-
dc.identifier.issn1525-0016-
dc.identifier.urihttp://hdl.handle.net/10722/194120-
dc.description.abstractThe U6 expression system was explored for efficient expression of a ribozyme against the human proto-oncogene c-neu. A hammerhead ribozyme (neuRz) and the control mutant ribozyme (MRz) were targeted to cleave c-neu mRNA at the tyrosine kinase domain. In vitro cleavage showed that neuRz was very active while MRz was not. Near-maximal target cleavage observed at a low ribozyme:target ratio (0.1) suggests that neuRz has good activity and turnover capability under physiological conditions, i.e., <5 mM MgCI2 and 37°C. Chimeric U6 ribozyme was expressed at about 5 × 106 copies/cell at 48 h in the ovarian carcinoma cell line SKOV-3.ip1. Partial down-regulation of c-neu mRNA and protein was observed in a dose-dependent manner in cells transiently transfected with U6neuRz- but not with MRz-containing plasmid. Sorted transient transfectants demonstrated dramatic growth inhibition with the neuRz-expressing cells. Our results demonstrate that the U6 expression system is very efficient and suitable for the expression of a hammerhead ribozyme. Moreover, nonviral delivery of the neuRz-expressing plasmid resulted in specific down-regulation of c-neu and, subsequently, growth inhibition of ovarian cancer cells overexpressing c-neu.-
dc.languageeng-
dc.relation.ispartofMolecular Therapy-
dc.titleSpecific down-regulation of HER-2/neu mediated by a chimeric U6 hammerhead ribozyme results in growth inhibition of human ovarian carcinoma-
dc.typeArticle-
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1006/mthe.2000.0241-
dc.identifier.pmid11237673-
dc.identifier.scopuseid_2-s2.0-0034986068-
dc.identifier.volume3-
dc.identifier.issue2-
dc.identifier.spage169-
dc.identifier.epage177-
dc.identifier.isiWOS:000167343800006-

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