File Download
There are no files associated with this item.
Supplementary
-
Citations:
- Appears in Collections:
Conference Paper: Aberrant DNA Double-strand break repair in the ovarian cancer cell line OVCAR-8
Title | Aberrant DNA Double-strand break repair in the ovarian cancer cell line OVCAR-8 |
---|---|
Authors | |
Issue Date | 2006 |
Publisher | American Association for Cancer Research |
Citation | AACR 97th Annual Meeting, Washington, DC, 1–5 April 2006. In Cancer Research, 2006, v. 66 n. 8S, p. 189 Abstract no. 803 How to Cite? |
Abstract | Aberrant repair of DNA double strand breaks (DSBs) is thought to be important in the generation of gross chromosomal rearrangements. To examine how DSBs might lead to chromosomal rearrangements, we investigated the consequences of the repair of a single DSB in the human ovarian cancer cell line OVCAR-8. An I-SceI recognition site was introduced into chromosome 2 of the recipient cells. This 18-bp sequence was inserted between a constitutive promoter (EF1α) and the Herpes simplex virus thymidine kinase (Hstk) gene which confers sensitivity to gancyclovir (GCV). Transfection of an I-SceI expression vector caused a single DSB in a subset of cells; clones that had aberrant repair and separated the EF1α promoter from the Hstk gene were resistant to GCV treatment. The most common mutations were interstitial deletions flanking the I-SceI cleavage region. More complex rearrangements, including chromosomal insertions derived from distant chromosomes regions, were also detected in some clones. This model system provides an ideal tool to investigate the mechanism of DNA repair in human tumor cells. |
Persistent Identifier | http://hdl.handle.net/10722/194043 |
ISSN | 2023 Impact Factor: 12.5 2023 SCImago Journal Rankings: 3.468 |
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Cheng, Y | en_US |
dc.contributor.author | Varga, T | en_US |
dc.contributor.author | Aplan, PD | en_US |
dc.date.accessioned | 2014-01-28T08:20:00Z | - |
dc.date.available | 2014-01-28T08:20:00Z | - |
dc.date.issued | 2006 | en_US |
dc.identifier.citation | AACR 97th Annual Meeting, Washington, DC, 1–5 April 2006. In Cancer Research, 2006, v. 66 n. 8S, p. 189 Abstract no. 803 | en_US |
dc.identifier.issn | 0008-5472 | - |
dc.identifier.uri | http://hdl.handle.net/10722/194043 | - |
dc.description.abstract | Aberrant repair of DNA double strand breaks (DSBs) is thought to be important in the generation of gross chromosomal rearrangements. To examine how DSBs might lead to chromosomal rearrangements, we investigated the consequences of the repair of a single DSB in the human ovarian cancer cell line OVCAR-8. An I-SceI recognition site was introduced into chromosome 2 of the recipient cells. This 18-bp sequence was inserted between a constitutive promoter (EF1α) and the Herpes simplex virus thymidine kinase (Hstk) gene which confers sensitivity to gancyclovir (GCV). Transfection of an I-SceI expression vector caused a single DSB in a subset of cells; clones that had aberrant repair and separated the EF1α promoter from the Hstk gene were resistant to GCV treatment. The most common mutations were interstitial deletions flanking the I-SceI cleavage region. More complex rearrangements, including chromosomal insertions derived from distant chromosomes regions, were also detected in some clones. This model system provides an ideal tool to investigate the mechanism of DNA repair in human tumor cells. | - |
dc.language | eng | en_US |
dc.publisher | American Association for Cancer Research | - |
dc.relation.ispartof | Cancer Research | en_US |
dc.title | Aberrant DNA Double-strand break repair in the ovarian cancer cell line OVCAR-8 | en_US |
dc.type | Conference_Paper | en_US |
dc.identifier.email | Cheng, Y: yuecheng@hku.hk | en_US |
dc.identifier.authority | Cheng, Y=rp01320 | en_US |
dc.publisher.place | Washington DC, USA | en_US |
dc.identifier.issnl | 0008-5472 | - |