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Conference Paper: Aberrant DNA Double-strand break repair in the ovarian cancer cell line OVCAR-8

TitleAberrant DNA Double-strand break repair in the ovarian cancer cell line OVCAR-8
Authors
Issue Date2006
PublisherAmerican Association for Cancer Research
Citation
AACR 97th Annual Meeting, Washington, DC, 1–5 April 2006. In Cancer Research, 2006, v. 66 n. 8S, p. 189 Abstract no. 803 How to Cite?
AbstractAberrant repair of DNA double strand breaks (DSBs) is thought to be important in the generation of gross chromosomal rearrangements. To examine how DSBs might lead to chromosomal rearrangements, we investigated the consequences of the repair of a single DSB in the human ovarian cancer cell line OVCAR-8. An I-SceI recognition site was introduced into chromosome 2 of the recipient cells. This 18-bp sequence was inserted between a constitutive promoter (EF1α) and the Herpes simplex virus thymidine kinase (Hstk) gene which confers sensitivity to gancyclovir (GCV). Transfection of an I-SceI expression vector caused a single DSB in a subset of cells; clones that had aberrant repair and separated the EF1α promoter from the Hstk gene were resistant to GCV treatment. The most common mutations were interstitial deletions flanking the I-SceI cleavage region. More complex rearrangements, including chromosomal insertions derived from distant chromosomes regions, were also detected in some clones. This model system provides an ideal tool to investigate the mechanism of DNA repair in human tumor cells.
Persistent Identifierhttp://hdl.handle.net/10722/194043
ISSN
2015 Impact Factor: 8.556
2015 SCImago Journal Rankings: 5.372

 

DC FieldValueLanguage
dc.contributor.authorCheng, Yen_US
dc.contributor.authorVarga, Ten_US
dc.contributor.authorAplan, PDen_US
dc.date.accessioned2014-01-28T08:20:00Z-
dc.date.available2014-01-28T08:20:00Z-
dc.date.issued2006en_US
dc.identifier.citationAACR 97th Annual Meeting, Washington, DC, 1–5 April 2006. In Cancer Research, 2006, v. 66 n. 8S, p. 189 Abstract no. 803en_US
dc.identifier.issn0008-5472-
dc.identifier.urihttp://hdl.handle.net/10722/194043-
dc.description.abstractAberrant repair of DNA double strand breaks (DSBs) is thought to be important in the generation of gross chromosomal rearrangements. To examine how DSBs might lead to chromosomal rearrangements, we investigated the consequences of the repair of a single DSB in the human ovarian cancer cell line OVCAR-8. An I-SceI recognition site was introduced into chromosome 2 of the recipient cells. This 18-bp sequence was inserted between a constitutive promoter (EF1α) and the Herpes simplex virus thymidine kinase (Hstk) gene which confers sensitivity to gancyclovir (GCV). Transfection of an I-SceI expression vector caused a single DSB in a subset of cells; clones that had aberrant repair and separated the EF1α promoter from the Hstk gene were resistant to GCV treatment. The most common mutations were interstitial deletions flanking the I-SceI cleavage region. More complex rearrangements, including chromosomal insertions derived from distant chromosomes regions, were also detected in some clones. This model system provides an ideal tool to investigate the mechanism of DNA repair in human tumor cells.-
dc.languageengen_US
dc.publisherAmerican Association for Cancer Research-
dc.relation.ispartofCancer Researchen_US
dc.titleAberrant DNA Double-strand break repair in the ovarian cancer cell line OVCAR-8en_US
dc.typeConference_Paperen_US
dc.identifier.emailCheng, Y: yuecheng@hku.hken_US
dc.identifier.authorityCheng, Y=rp01320en_US
dc.publisher.placeWashington DC, USAen_US

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