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Article: Purpurin Triggers Caspase-Independent Apoptosis in Candida dubliniensis Biofilms

TitlePurpurin Triggers Caspase-Independent Apoptosis in Candida dubliniensis Biofilms
Authors
Issue Date2013
PublisherPublic Library of Science. The Journal's web site is located at http://www.plosone.org/home.action
Citation
PLoS One, 2013, v. 8 n. 12, p. article no. e86032 How to Cite?
AbstractCandida dubliniensis is an important human fungal pathogen that causes oral infections in patients with AIDS and diabetes mellitus. However, C. Dubliniensis has been frequently reported in bloodstream infections in clinical settings. Like its phylogenetically related virulent species C. albicans, C. Dubliniensis is able to grow and switch between yeast form and filamentous form (hyphae) and develops biofilms on both abiotic and biotic surfaces. Biofilms are recalcitrant to antifungal therapies and C. Dubliniensis readily turns drug resistant upon repeated exposure. More than 80% of infections are associated with biofilms. Suppression of fungal biofilms may therefore represent a viable antifungal strategy with clinical relevance. Here, we report that C. dubliniensis biofilms were inhibited by purpurin, a natural anthraquinone pigment isolated from madder root. Purpurin inhibited C. dubliniensis biofilm formation in a concentration-dependent manner; while mature biofilms were less susceptible to purpurin. Scanning electron microscopy (SEM) analysis revealed scanty structure consisting of yeast cells in purpurin-treated C. dubliniensis biofilms. We sought to delineate the mechanisms of the anti-biofilm activity of purpurin on C. Dubliniensis. Intracellular ROS levels were significantly elevated in fungal biofilms and depolarization of MMP was evident upon purpurin treatment in a concentration-dependent manner. DNA degradation was evident. However, no activated metacaspase could be detected. Together, purpurin triggered metacaspase-independent apoptosis in C. dubliniensis biofilms.
Persistent Identifierhttp://hdl.handle.net/10722/193576
ISSN
2015 Impact Factor: 3.057
2015 SCImago Journal Rankings: 1.395
PubMed Central ID
ISI Accession Number ID

 

DC FieldValueLanguage
dc.contributor.authorTsang, WKen_US
dc.contributor.authorWong, PKen_US
dc.contributor.authorYang, Hen_US
dc.contributor.authorLi, NFen_US
dc.date.accessioned2014-01-20T05:04:54Z-
dc.date.available2014-01-20T05:04:54Z-
dc.date.issued2013en_US
dc.identifier.citationPLoS One, 2013, v. 8 n. 12, p. article no. e86032en_US
dc.identifier.issn1932-6203en_US
dc.identifier.urihttp://hdl.handle.net/10722/193576-
dc.description.abstractCandida dubliniensis is an important human fungal pathogen that causes oral infections in patients with AIDS and diabetes mellitus. However, C. Dubliniensis has been frequently reported in bloodstream infections in clinical settings. Like its phylogenetically related virulent species C. albicans, C. Dubliniensis is able to grow and switch between yeast form and filamentous form (hyphae) and develops biofilms on both abiotic and biotic surfaces. Biofilms are recalcitrant to antifungal therapies and C. Dubliniensis readily turns drug resistant upon repeated exposure. More than 80% of infections are associated with biofilms. Suppression of fungal biofilms may therefore represent a viable antifungal strategy with clinical relevance. Here, we report that C. dubliniensis biofilms were inhibited by purpurin, a natural anthraquinone pigment isolated from madder root. Purpurin inhibited C. dubliniensis biofilm formation in a concentration-dependent manner; while mature biofilms were less susceptible to purpurin. Scanning electron microscopy (SEM) analysis revealed scanty structure consisting of yeast cells in purpurin-treated C. dubliniensis biofilms. We sought to delineate the mechanisms of the anti-biofilm activity of purpurin on C. Dubliniensis. Intracellular ROS levels were significantly elevated in fungal biofilms and depolarization of MMP was evident upon purpurin treatment in a concentration-dependent manner. DNA degradation was evident. However, no activated metacaspase could be detected. Together, purpurin triggered metacaspase-independent apoptosis in C. dubliniensis biofilms.-
dc.languageengen_US
dc.publisherPublic Library of Science. The Journal's web site is located at http://www.plosone.org/home.actionen_US
dc.relation.ispartofPLoS Oneen_US
dc.rightsCreative Commons: Attribution 3.0 Hong Kong License-
dc.titlePurpurin Triggers Caspase-Independent Apoptosis in Candida dubliniensis Biofilmsen_US
dc.typeArticleen_US
dc.identifier.emailTsang, WK: pwktsang@hku.hken_US
dc.identifier.emailWong, PK: alanpkw@hku.hken_US
dc.identifier.emailLi, NF: ngaifor@hku.hken_US
dc.identifier.authorityTsang, WK=rp01388en_US
dc.description.naturepublished_or_final_version-
dc.identifier.doi10.1371/journal.pone.0086032-
dc.identifier.pmid24376900-
dc.identifier.pmcidPMC3871571-
dc.identifier.hkuros227183en_US
dc.identifier.volume8-
dc.identifier.issue12-
dc.identifier.spagearticle no. e86032-
dc.identifier.epagearticle no. e86032-
dc.identifier.isiWOS:000328882000195-
dc.publisher.placeUnited Statesen_US

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