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postgraduate thesis: Identification of pathogenic fungal isolates by ITS sequencing

TitleIdentification of pathogenic fungal isolates by ITS sequencing
Authors
Issue Date2013
PublisherThe University of Hong Kong (Pokfulam, Hong Kong)
Citation
Lau, C. [劉清麗]. (2013). Identification of pathogenic fungal isolates by ITS sequencing. (Thesis). University of Hong Kong, Pokfulam, Hong Kong SAR. Retrieved from http://dx.doi.org/10.5353/th_b5091411
AbstractIn clinical microbiology laboratories, the conventional method for identification of pathogenic fungi is based on fungal culture and observation of fungal phenotypic characters. However, it is time-consuming, subjective and unreliable due to the long incubation period and variations in fungal colony morphology. Thus, there is a need for a rapid, objective and accurate identification of pathogenic fungal isolates. ITS regions are most commonly used targets for molecular identification of fungal pathogens because of the optimal inter- and intra-species variations and large copies in fungal genome. In this study, twenty-two clinical fungal isolates were identified using the phenotypic method and ITS sequencing. The results showed that there were only thirteen isolates identified to species level by phenotypic method, while others were only differentiated in genus level. Due to the poor differentiation based on the conventional phenotypic approach, misidentification of fungal pathogens occasionally occurred. However, ITS sequencing successfully achieved accurate species-level identification of all fungal isolates. The results were demonstrated in phylogenetic trees with high bootstrap support. In conclusion, ITS sequencing is a rapid and reliable for the identification of pathogenic fungal isolates.
DegreeMaster of Medical Sciences
SubjectPathogenic fungi - Identification
Dept/ProgramMicrobiology
Persistent Identifierhttp://hdl.handle.net/10722/193540

 

DC FieldValueLanguage
dc.contributor.authorLau, Ching-lai-
dc.contributor.author劉清麗-
dc.date.accessioned2014-01-13T23:10:36Z-
dc.date.available2014-01-13T23:10:36Z-
dc.date.issued2013-
dc.identifier.citationLau, C. [劉清麗]. (2013). Identification of pathogenic fungal isolates by ITS sequencing. (Thesis). University of Hong Kong, Pokfulam, Hong Kong SAR. Retrieved from http://dx.doi.org/10.5353/th_b5091411-
dc.identifier.urihttp://hdl.handle.net/10722/193540-
dc.description.abstractIn clinical microbiology laboratories, the conventional method for identification of pathogenic fungi is based on fungal culture and observation of fungal phenotypic characters. However, it is time-consuming, subjective and unreliable due to the long incubation period and variations in fungal colony morphology. Thus, there is a need for a rapid, objective and accurate identification of pathogenic fungal isolates. ITS regions are most commonly used targets for molecular identification of fungal pathogens because of the optimal inter- and intra-species variations and large copies in fungal genome. In this study, twenty-two clinical fungal isolates were identified using the phenotypic method and ITS sequencing. The results showed that there were only thirteen isolates identified to species level by phenotypic method, while others were only differentiated in genus level. Due to the poor differentiation based on the conventional phenotypic approach, misidentification of fungal pathogens occasionally occurred. However, ITS sequencing successfully achieved accurate species-level identification of all fungal isolates. The results were demonstrated in phylogenetic trees with high bootstrap support. In conclusion, ITS sequencing is a rapid and reliable for the identification of pathogenic fungal isolates.-
dc.languageeng-
dc.publisherThe University of Hong Kong (Pokfulam, Hong Kong)-
dc.relation.ispartofHKU Theses Online (HKUTO)-
dc.rightsThe author retains all proprietary rights, (such as patent rights) and the right to use in future works.-
dc.rightsCreative Commons: Attribution 3.0 Hong Kong License-
dc.subject.lcshPathogenic fungi - Identification-
dc.titleIdentification of pathogenic fungal isolates by ITS sequencing-
dc.typePG_Thesis-
dc.identifier.hkulb5091411-
dc.description.thesisnameMaster of Medical Sciences-
dc.description.thesislevelMaster-
dc.description.thesisdisciplineMicrobiology-
dc.description.naturepublished_or_final_version-
dc.identifier.doi10.5353/th_b5091411-
dc.date.hkucongregation2013-

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