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Article: Gastrointestinal bacteria generate nitric oxide from nitrate and nitrite

TitleGastrointestinal bacteria generate nitric oxide from nitrate and nitrite
Authors
Issue Date2005
Citation
Nitric Oxide - Biology and Chemistry, 2005, v. 13 n. 4, p. 272-278 How to Cite?
AbstractDenitrifying bacteria in soil generate nitric oxide (NO) from nitrite as a part of the nitrogen cycle, but little is known about NO production by commensal bacteria. We used a chemiluminescence assay to explore if human faeces and different representative gut bacteria are able to generate NO. Bacteria were incubated anaerobically in gas-tight bags, with or without nitrate or nitrite in the growth medium. In addition, luminal NO levels were measured in vivo in the intestines in germ-free and conventional rats, and in rats mono-associated with lactobacilli. We show that human faeces can generate NO after nitrate or nitrite supplementation. Lactobacilli and bifidobacteria generated much NO from nitrite, but only a few of the tested strains produced NO from nitrate and at much lower levels. In contrast, Escherichia coli, Bacteroides thetaiotaomicron, and Clostridium difficile did not produce significant amounts of NO either with nitrate or nitrite. NO generation in the gut lumen was also observed in vivo in conventional rats but not in germ-free rats or in rats mono-associated with lactobacilli. We conclude that NO can be generated by the anaerobic gut flora in the presence of nitrate or nitrite. Future studies will reveal its biological significance in regulation of gastrointestinal integrity. © 2005 Elsevier Inc. All rights reserved.
Persistent Identifierhttp://hdl.handle.net/10722/192694
ISSN
2015 Impact Factor: 3.76
2015 SCImago Journal Rankings: 1.292
ISI Accession Number ID

 

DC FieldValueLanguage
dc.contributor.authorSobko, Ten_US
dc.contributor.authorReinders, CIen_US
dc.contributor.authorJansson, EÅen_US
dc.contributor.authorNorin, Een_US
dc.contributor.authorMidtvedt, Ten_US
dc.contributor.authorLundberg, JOen_US
dc.date.accessioned2013-11-20T04:56:01Z-
dc.date.available2013-11-20T04:56:01Z-
dc.date.issued2005en_US
dc.identifier.citationNitric Oxide - Biology and Chemistry, 2005, v. 13 n. 4, p. 272-278en_US
dc.identifier.issn1089-8603en_US
dc.identifier.urihttp://hdl.handle.net/10722/192694-
dc.description.abstractDenitrifying bacteria in soil generate nitric oxide (NO) from nitrite as a part of the nitrogen cycle, but little is known about NO production by commensal bacteria. We used a chemiluminescence assay to explore if human faeces and different representative gut bacteria are able to generate NO. Bacteria were incubated anaerobically in gas-tight bags, with or without nitrate or nitrite in the growth medium. In addition, luminal NO levels were measured in vivo in the intestines in germ-free and conventional rats, and in rats mono-associated with lactobacilli. We show that human faeces can generate NO after nitrate or nitrite supplementation. Lactobacilli and bifidobacteria generated much NO from nitrite, but only a few of the tested strains produced NO from nitrate and at much lower levels. In contrast, Escherichia coli, Bacteroides thetaiotaomicron, and Clostridium difficile did not produce significant amounts of NO either with nitrate or nitrite. NO generation in the gut lumen was also observed in vivo in conventional rats but not in germ-free rats or in rats mono-associated with lactobacilli. We conclude that NO can be generated by the anaerobic gut flora in the presence of nitrate or nitrite. Future studies will reveal its biological significance in regulation of gastrointestinal integrity. © 2005 Elsevier Inc. All rights reserved.en_US
dc.languageengen_US
dc.relation.ispartofNitric Oxide - Biology and Chemistryen_US
dc.titleGastrointestinal bacteria generate nitric oxide from nitrate and nitriteen_US
dc.typeArticleen_US
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.doi10.1016/j.niox.2005.08.002en_US
dc.identifier.pmid16183308-
dc.identifier.scopuseid_2-s2.0-27644473418en_US
dc.identifier.volume13en_US
dc.identifier.issue4en_US
dc.identifier.spage272en_US
dc.identifier.epage278en_US
dc.identifier.isiWOS:000233504500008-

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