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Conference Paper: Hydroxyapatite regeneration of human tooth by hydrogel biomimetic mineralization model

TitleHydroxyapatite regeneration of human tooth by hydrogel biomimetic mineralization model
Authors
KeywordsDentin
Enamel
Mineralization
Regeneration and hydrogel
Issue Date2013
PublisherSage Publications, Inc. The Journal's web site is located at http://www.sagepub.com/journalsProdDesc.nav?prodId=Journal201925
Citation
The 2nd Meeting of the International Association of Dental Research - Asia Pacific Region (IADR-APR), Bangkok, Thailand, 21-23 August 2013. In Journal of Dental Research, 2013, v. 92 n. Special Issue B: abstract no. 361 How to Cite?
AbstractObjective: To describe the effects of a novel hydrogel biomimetic mineralization model in regulating hydroxyapatite regeneration on demineralized human tooth surface. Methods: Human tooth slices with enamel and dentine were prepared and polished smooth. They were demineralized by etching with 37% phosphoric acid for 15 second. The etched surface was covered by a 2mm layer of calcium chloride agarose hydrogel. Another 2mm layer of calcium ion free agarose hydrogel was added on top of the calcium chloride agarose hydrogel. They were incubated at 37oC with a freshly prepared and acidity neutral solution containing sodium hydrogen phosphate and sodium fluoride. The solution was replaced every 24 hours; and the two layer of agarose hydrogel was changed every 48 hours. Scanning electron microscopy (SEM) was used to study the crystals regenerated on enamel and dentine surface. X-ray diffraction (XRD) and Fourier transform infrared spectroscopy (FTIR) were used to evaluate the chemical structure of the crystals. Results: SEM showed that a dense layer of crystals was regenerated and uniformly covered the enamel and dentine surface. Higher magnification (50,000x) showed that the regenerated crystals had a typical apatite hexagonal structure with the size of approximately 150nm in diameter and 2µm in length. They were orderly formed in parallel bundles. XRD and FTIR analysis suggested that the crystals were hydroxyapatite. Conclusion: In this study, hydroxyapatite was regenerated on the demineralized enamel and dentine surface by a novel hydrogel biomimetic mineralization model. (Supported by NSFC-RGC Grant N_HKU776/10) This abstract is based on research that was funded entirely or partially by an outside source: NSFC-RGC Grant N_HKU776/10
DescriptionConference Theme: We are the Future
Oral Presentation
Session 13: O-SEA Unilever competition (Senior)
Persistent Identifierhttp://hdl.handle.net/10722/192576
ISSN
2015 Impact Factor: 4.602
2015 SCImago Journal Rankings: 1.714

 

DC FieldValueLanguage
dc.contributor.authorCao, Yen_US
dc.contributor.authorMei, Len_US
dc.contributor.authorLi, QLen_US
dc.contributor.authorChu, CHen_US
dc.contributor.authorLo, ECMen_US
dc.date.accessioned2013-11-18T05:06:46Z-
dc.date.available2013-11-18T05:06:46Z-
dc.date.issued2013en_US
dc.identifier.citationThe 2nd Meeting of the International Association of Dental Research - Asia Pacific Region (IADR-APR), Bangkok, Thailand, 21-23 August 2013. In Journal of Dental Research, 2013, v. 92 n. Special Issue B: abstract no. 361en_US
dc.identifier.issn0022-0345-
dc.identifier.urihttp://hdl.handle.net/10722/192576-
dc.descriptionConference Theme: We are the Future-
dc.descriptionOral Presentation-
dc.descriptionSession 13: O-SEA Unilever competition (Senior)-
dc.description.abstractObjective: To describe the effects of a novel hydrogel biomimetic mineralization model in regulating hydroxyapatite regeneration on demineralized human tooth surface. Methods: Human tooth slices with enamel and dentine were prepared and polished smooth. They were demineralized by etching with 37% phosphoric acid for 15 second. The etched surface was covered by a 2mm layer of calcium chloride agarose hydrogel. Another 2mm layer of calcium ion free agarose hydrogel was added on top of the calcium chloride agarose hydrogel. They were incubated at 37oC with a freshly prepared and acidity neutral solution containing sodium hydrogen phosphate and sodium fluoride. The solution was replaced every 24 hours; and the two layer of agarose hydrogel was changed every 48 hours. Scanning electron microscopy (SEM) was used to study the crystals regenerated on enamel and dentine surface. X-ray diffraction (XRD) and Fourier transform infrared spectroscopy (FTIR) were used to evaluate the chemical structure of the crystals. Results: SEM showed that a dense layer of crystals was regenerated and uniformly covered the enamel and dentine surface. Higher magnification (50,000x) showed that the regenerated crystals had a typical apatite hexagonal structure with the size of approximately 150nm in diameter and 2µm in length. They were orderly formed in parallel bundles. XRD and FTIR analysis suggested that the crystals were hydroxyapatite. Conclusion: In this study, hydroxyapatite was regenerated on the demineralized enamel and dentine surface by a novel hydrogel biomimetic mineralization model. (Supported by NSFC-RGC Grant N_HKU776/10) This abstract is based on research that was funded entirely or partially by an outside source: NSFC-RGC Grant N_HKU776/10-
dc.languageengen_US
dc.publisherSage Publications, Inc. The Journal's web site is located at http://www.sagepub.com/journalsProdDesc.nav?prodId=Journal201925-
dc.relation.ispartofJournal of Dental Researchen_US
dc.rightsJournal of Dental Research. Copyright © Sage Publications, Inc.-
dc.subjectDentin-
dc.subjectEnamel-
dc.subjectMineralization-
dc.subjectRegeneration and hydrogel-
dc.titleHydroxyapatite regeneration of human tooth by hydrogel biomimetic mineralization modelen_US
dc.typeConference_Paperen_US
dc.identifier.emailMei, L: mei1123@hku.hken_US
dc.identifier.emailChu, CH: chchu@hku.hken_US
dc.identifier.emailLo, ECM: hrdplcm@hkucc.hku.hken_US
dc.identifier.authorityMei, L=rp01840en_US
dc.identifier.authorityChu, CH=rp00022en_US
dc.identifier.authorityLo, ECM=rp00015en_US
dc.identifier.hkuros226806en_US
dc.identifier.hkuros226765-
dc.identifier.volume92en_US
dc.identifier.issueSpecial Issue B: abstract no. 361en_US
dc.publisher.placeUnited States-

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