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Conference Paper: Common and Specific Dental Plaque Microbiomes of Human and Giant-Panda

TitleCommon and Specific Dental Plaque Microbiomes of Human and Giant-Panda
Authors
KeywordsAnimal
Biofilm
Microbiology and Nucleic acids
Issue Date2013
PublisherSage Publications, Inc. The Journal's web site is located at http://www.sagepub.com/journalsProdDesc.nav?prodId=Journal201925
Citation
The 2nd Meeting of the International Association of Dental Research - Asia Pacific Region (IADR-APR), Bangkok, Thailand, 21-23 August 2013. In Journal of Dental Research, 2013, v. 92 n. Special Issue B: abstract no. 724 How to Cite?
AbstractObjective: Dental plaque microbiome is the aetiological agent for dental caries and periodontal diseases in humans and animals. Previously we reported the first metagenomics data of animal dental plaque by presenting Giant Panda (Ailuropoda melanoleuca) dental plaque microbiome (IADR General sessions, Seattle, 2013). In this study, we compared the complexity of Giant Panda and Human dental plaque microbiomes utilizing the Human Oral Microbiome Database (HOMD). Method: Dental plaque samples were collected from healthy, caries-free, gingivitis-free sites (GP-H) and sites with gingivitis (GP-D) for metagenomics DNA sequencing using the Illumina and 454-Titanium platforms. Microbial identification and taxonomy were obtained using NCBI NR database and MEGAN/MG-RAST, respectively. Relative abundance and statistical analysis of bacterial, fungi and protozoa specific sequence reads between healthy and diseased samples were obtained by aligning reads to the corresponding NCBI RefSeq database using Novoalign. Bacterial complexities were further evaluated against HOMD to identify Human-specific and Panda-specific dental plaque microbiota. Results: Approximately 16% of reads come from non-Panda species, with 110 bacteria, 14 fungi and 17 Protozoa identified at genus level.Fungi of the Giant Panda dental plaque microbiome include: Penicillium, Aspergillus, and Candida species. Comparison with HOMD revealed 182 common bacterial species shared between the Giant Panda and Human dental plaque microbiomes (e.g. Streptococcus, Porphyromonas). Some species were Panda-specific (Psychrobacter, Ramlibacter). Conclusion: The present study compares Human and Giant Panda dental plaque microbiomes for the first time and identified common and species-specific dental plaque components. This metagenomics data will be a useful resource for further inter-species comparative studies that will shed more light on the pathogenic potential of dental plaque microbiome. This abstract is based on research that was funded entirely or partially by an outside source: This study was funded by HKU Research Output Prize to CJS and Endowed Professorship to LJJ
DescriptionConference Theme: We are the Future
Oral Presentation
Session 26: O17
Persistent Identifierhttp://hdl.handle.net/10722/192569
ISSN
2015 Impact Factor: 4.602
2015 SCImago Journal Rankings: 1.714

 

DC FieldValueLanguage
dc.contributor.authorSeneviratne, CJen_US
dc.contributor.authorLok, Sen_US
dc.contributor.authorTong, Aen_US
dc.contributor.authorKot, CCSen_US
dc.contributor.authorMartelli, Pen_US
dc.contributor.authorChan, TFen_US
dc.contributor.authorYim, AKen_US
dc.contributor.authorYu, Cen_US
dc.contributor.authorZhu, Len_US
dc.contributor.authorSamaranayake, LPen_US
dc.contributor.authorJin, Len_US
dc.date.accessioned2013-11-18T05:06:42Z-
dc.date.available2013-11-18T05:06:42Z-
dc.date.issued2013en_US
dc.identifier.citationThe 2nd Meeting of the International Association of Dental Research - Asia Pacific Region (IADR-APR), Bangkok, Thailand, 21-23 August 2013. In Journal of Dental Research, 2013, v. 92 n. Special Issue B: abstract no. 724en_US
dc.identifier.issn0022-0345-
dc.identifier.urihttp://hdl.handle.net/10722/192569-
dc.descriptionConference Theme: We are the Future-
dc.descriptionOral Presentation-
dc.descriptionSession 26: O17-
dc.description.abstractObjective: Dental plaque microbiome is the aetiological agent for dental caries and periodontal diseases in humans and animals. Previously we reported the first metagenomics data of animal dental plaque by presenting Giant Panda (Ailuropoda melanoleuca) dental plaque microbiome (IADR General sessions, Seattle, 2013). In this study, we compared the complexity of Giant Panda and Human dental plaque microbiomes utilizing the Human Oral Microbiome Database (HOMD). Method: Dental plaque samples were collected from healthy, caries-free, gingivitis-free sites (GP-H) and sites with gingivitis (GP-D) for metagenomics DNA sequencing using the Illumina and 454-Titanium platforms. Microbial identification and taxonomy were obtained using NCBI NR database and MEGAN/MG-RAST, respectively. Relative abundance and statistical analysis of bacterial, fungi and protozoa specific sequence reads between healthy and diseased samples were obtained by aligning reads to the corresponding NCBI RefSeq database using Novoalign. Bacterial complexities were further evaluated against HOMD to identify Human-specific and Panda-specific dental plaque microbiota. Results: Approximately 16% of reads come from non-Panda species, with 110 bacteria, 14 fungi and 17 Protozoa identified at genus level.Fungi of the Giant Panda dental plaque microbiome include: Penicillium, Aspergillus, and Candida species. Comparison with HOMD revealed 182 common bacterial species shared between the Giant Panda and Human dental plaque microbiomes (e.g. Streptococcus, Porphyromonas). Some species were Panda-specific (Psychrobacter, Ramlibacter). Conclusion: The present study compares Human and Giant Panda dental plaque microbiomes for the first time and identified common and species-specific dental plaque components. This metagenomics data will be a useful resource for further inter-species comparative studies that will shed more light on the pathogenic potential of dental plaque microbiome. This abstract is based on research that was funded entirely or partially by an outside source: This study was funded by HKU Research Output Prize to CJS and Endowed Professorship to LJJ-
dc.languageengen_US
dc.publisherSage Publications, Inc. The Journal's web site is located at http://www.sagepub.com/journalsProdDesc.nav?prodId=Journal201925-
dc.relation.ispartofJournal of Dental Researchen_US
dc.rightsJournal of Dental Research. Copyright © Sage Publications, Inc.-
dc.subjectAnimal-
dc.subjectBiofilm-
dc.subjectMicrobiology and Nucleic acids-
dc.titleCommon and Specific Dental Plaque Microbiomes of Human and Giant-Pandaen_US
dc.typeConference_Paperen_US
dc.identifier.emailSeneviratne, CJ: jaya@hku.hken_US
dc.identifier.emailLok, S: silok@genome.hku.hken_US
dc.identifier.emailKot, CCS: scckot@hku.hken_US
dc.identifier.emailSamaranayake, LP: lakshman@hku.hken_US
dc.identifier.emailJin, L: ljjin@hkucc.hku.hken_US
dc.identifier.authoritySeneviratne, CJ=rp01372en_US
dc.identifier.authorityLok, S=rp00271en_US
dc.identifier.authoritySamaranayake, LP=rp00023en_US
dc.identifier.authorityJin, L=rp00028en_US
dc.identifier.hkuros226799en_US
dc.identifier.volume92en_US
dc.identifier.issueSpecial Issue B: abstract no. 724en_US
dc.publisher.placeUnited States-

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