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Article: Adipose stem cell coating of biomimetic β-TCP macrospheres by use of a laboratory centrifuge

TitleAdipose stem cell coating of biomimetic β-TCP macrospheres by use of a laboratory centrifuge
Authors
KeywordsAdipose-derived stem cells
Biomimetic scaffolds
Cell seeding
Scaffold coating
Stem cell coating
Issue Date2013
PublisherMary Ann Liebert, Inc. Publishers. The Journal's web site is located at http://www.liebertpub.com/overview/bioresearch-open-access/609/
Citation
BioResearch Open Access, 2013, v. 2 n. 1, p. 67-71 How to Cite?
AbstractBiomimetic materials such as coral exoskeletons possess unique architectural structures with a uniform and interconnected porous network that can be beneficial as a scaffold material. In addition, these marine structures can be hydrothermally converted to calcium phosphates, while retaining the original structural properties. The ability of biomaterials to stimulate the local microenvironment is one of the main focuses in tissue engineering, and directly coating the scaffold with stem cells facilitates future potential applications in therapeutics and regenerative medicine. In this article we describe a new and simple method that uses a laboratory centrifuge to coat hydrothermally derived beta-tricalcium phosphate macrospheres from coral exoskeleton with stem cells. In this research the optimal seeding duration and speed were determined to be 1 min and 700 g. Scanning electron micrographs showed complete surface coverage by stem cells within 7 days of seeding. This study constitutes an important step toward achieving functional tissue-engineered implants by increasing our understanding of the influence of dynamic parameters on the efficiency and distribution of stem cell attachment to biomimetic materials and how stem cells interact with biomimetic materials.
Persistent Identifierhttp://hdl.handle.net/10722/192472
ISSN
PubMed Central ID

 

DC FieldValueLanguage
dc.contributor.authorChou, Jen_US
dc.contributor.authorGreen, DWen_US
dc.contributor.authorSingh, Ken_US
dc.contributor.authorHao, Jen_US
dc.contributor.authorBen-Nissan, Ben_US
dc.contributor.authorMilthorpe, Ben_US
dc.date.accessioned2013-11-15T02:13:36Z-
dc.date.available2013-11-15T02:13:36Z-
dc.date.issued2013en_US
dc.identifier.citationBioResearch Open Access, 2013, v. 2 n. 1, p. 67-71en_US
dc.identifier.issn2164-7860en_US
dc.identifier.urihttp://hdl.handle.net/10722/192472-
dc.description.abstractBiomimetic materials such as coral exoskeletons possess unique architectural structures with a uniform and interconnected porous network that can be beneficial as a scaffold material. In addition, these marine structures can be hydrothermally converted to calcium phosphates, while retaining the original structural properties. The ability of biomaterials to stimulate the local microenvironment is one of the main focuses in tissue engineering, and directly coating the scaffold with stem cells facilitates future potential applications in therapeutics and regenerative medicine. In this article we describe a new and simple method that uses a laboratory centrifuge to coat hydrothermally derived beta-tricalcium phosphate macrospheres from coral exoskeleton with stem cells. In this research the optimal seeding duration and speed were determined to be 1 min and 700 g. Scanning electron micrographs showed complete surface coverage by stem cells within 7 days of seeding. This study constitutes an important step toward achieving functional tissue-engineered implants by increasing our understanding of the influence of dynamic parameters on the efficiency and distribution of stem cell attachment to biomimetic materials and how stem cells interact with biomimetic materials.en_US
dc.languageengen_US
dc.publisherMary Ann Liebert, Inc. Publishers. The Journal's web site is located at http://www.liebertpub.com/overview/bioresearch-open-access/609/en_US
dc.relation.ispartofBioResearch Open Accessen_US
dc.rightsThis is a copy of an article published in the BioResearch Open Access © 2013 copyright Mary Ann Liebert, Inc.; BioResearch Open Access is available online at: http://www.liebertonline.com.en_US
dc.rightsCreative Commons: Attribution 3.0 Hong Kong Licenseen_US
dc.subjectAdipose-derived stem cellsen_US
dc.subjectBiomimetic scaffoldsen_US
dc.subjectCell seedingen_US
dc.subjectScaffold coatingen_US
dc.subjectStem cell coatingen_US
dc.titleAdipose stem cell coating of biomimetic β-TCP macrospheres by use of a laboratory centrifugeen_US
dc.typeArticleen_US
dc.identifier.emailGreen, DW: dwgreen@hku.hken_US
dc.identifier.authorityGreen, DW=rp01598en_US
dc.description.naturepublished_or_final_versionen_US
dc.identifier.doi10.1089/biores.2012.0269en_US
dc.identifier.pmid23515356en_US
dc.identifier.pmcidPMC3569929en_US
dc.identifier.hkuros226477en_US
dc.identifier.volume2en_US
dc.identifier.issue1en_US
dc.identifier.spage67en_US
dc.identifier.epage71en_US
dc.publisher.placeUnited Statesen_US

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