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Article: Biomimetic mineralisation of phosphorylated dentine by CPP-ACP

TitleBiomimetic mineralisation of phosphorylated dentine by CPP-ACP
Authors
Issue Date2013
Citation
Journal of Dentistry, 2013, v. 41 n. 9, p. 818-825 How to Cite?
AbstractObjectives Casein phosphopeptide-amorphous calcium phosphate (CPP-ACP) has the potential to induce the biomimetic mineralisation of dentine collagen fibrils. This study aimed to demonstrate in vitro the ability of CPP-ACP to form apatite crystals on phosphorylated dentine collagen fibrils. Methods Dentine slices with a 2-mm thickness were prepared from sound human third molars. The slices were etched with phosphoric acid to expose the collagen fibres. Sodium trimetaphosphate was then used to phosphorylate the exposed collagen fibres. CPP-ACP paste was topically applied to the surface of the phosphorylated slices, which were then immersed in a metastable calcium phosphate remineralising solution and incubated at 37 °C for 10 days. The CPP-ACP paste and the remineralising solution were replaced every two days. Phosphorylated dentine slices without a CPP-ACP application and non-phosphorylated dentine slices with a CPP-ACP application were prepared and used for comparison. The slices were examined using scanning electron microscope (SEM), diffuse reflectance-Fourier transform infrared spectroscopy (DR-FTIR) and X-ray diffraction (XRD). Results The SEM results revealed the presence of intrafibrillar and interfibrillar crystal nucleation and growth along the phosphorylated dentine collagen fibres. The DR-FTIR and XRD confirmed that the crystals were hydroxyapatite. No apatite crystal nucleation and growth were observed in either the slices that had no non-phosphorylation or those without CPP-ACP application. Conclusions CPP-ACP can induce the biomimetic mineralisation of dentine through apatite formation along and between the phosphorylated dentine collagen fibres. Clinical significance The in vitro study imitated the application of CPP-ACP to exposed dentine tooth surfaces in the mouth. This could lead to the development of a new therapeutic technique for the treatment of tooth hypersensitivity.
Persistent Identifierhttp://hdl.handle.net/10722/192320
ISSN
2015 Impact Factor: 3.109
2015 SCImago Journal Rankings: 1.029
ISI Accession Number ID

 

DC FieldValueLanguage
dc.contributor.authorCao, Yen_US
dc.contributor.authorMei, MLen_US
dc.contributor.authorXu, Jen_US
dc.contributor.authorLo, ECMen_US
dc.contributor.authorLi, Qen_US
dc.contributor.authorChu, CHen_US
dc.date.accessioned2013-10-24T01:49:30Z-
dc.date.available2013-10-24T01:49:30Z-
dc.date.issued2013en_US
dc.identifier.citationJournal of Dentistry, 2013, v. 41 n. 9, p. 818-825en_US
dc.identifier.issn0300-5712en_US
dc.identifier.urihttp://hdl.handle.net/10722/192320-
dc.description.abstractObjectives Casein phosphopeptide-amorphous calcium phosphate (CPP-ACP) has the potential to induce the biomimetic mineralisation of dentine collagen fibrils. This study aimed to demonstrate in vitro the ability of CPP-ACP to form apatite crystals on phosphorylated dentine collagen fibrils. Methods Dentine slices with a 2-mm thickness were prepared from sound human third molars. The slices were etched with phosphoric acid to expose the collagen fibres. Sodium trimetaphosphate was then used to phosphorylate the exposed collagen fibres. CPP-ACP paste was topically applied to the surface of the phosphorylated slices, which were then immersed in a metastable calcium phosphate remineralising solution and incubated at 37 °C for 10 days. The CPP-ACP paste and the remineralising solution were replaced every two days. Phosphorylated dentine slices without a CPP-ACP application and non-phosphorylated dentine slices with a CPP-ACP application were prepared and used for comparison. The slices were examined using scanning electron microscope (SEM), diffuse reflectance-Fourier transform infrared spectroscopy (DR-FTIR) and X-ray diffraction (XRD). Results The SEM results revealed the presence of intrafibrillar and interfibrillar crystal nucleation and growth along the phosphorylated dentine collagen fibres. The DR-FTIR and XRD confirmed that the crystals were hydroxyapatite. No apatite crystal nucleation and growth were observed in either the slices that had no non-phosphorylation or those without CPP-ACP application. Conclusions CPP-ACP can induce the biomimetic mineralisation of dentine through apatite formation along and between the phosphorylated dentine collagen fibres. Clinical significance The in vitro study imitated the application of CPP-ACP to exposed dentine tooth surfaces in the mouth. This could lead to the development of a new therapeutic technique for the treatment of tooth hypersensitivity.-
dc.languageengen_US
dc.relation.ispartofJournal of Dentistryen_US
dc.rightsNOTICE: this is the author’s version of a work that was accepted for publication in . Changes resulting from the publishing process, such as peer review, editing, corrections, structural formatting, and other quality control mechanisms may not be reflected in this document. Changes may have been made to this work since it was submitted for publication. A definitive version was subsequently published in Journal of Dentistry, [VOL 41, ISSUE 9, 2013] DOI 10.1016/j.jdent.2013.06.008-
dc.titleBiomimetic mineralisation of phosphorylated dentine by CPP-ACPen_US
dc.typeArticleen_US
dc.identifier.doi10.1016/j.jdent.2013.06.008en_US
dc.identifier.pmid23810733-
dc.identifier.scopuseid_2-s2.0-84882453003en_US
dc.identifier.hkuros226358-
dc.identifier.hkuros228343-
dc.identifier.volume41en_US
dc.identifier.issue9en_US
dc.identifier.spage818en_US
dc.identifier.epage825en_US
dc.identifier.isiWOS:000323767100009-

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