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Article: A novel oligopeptide simulating dentine matrix protein 1 for biomimetic mineralization of dentine

TitleA novel oligopeptide simulating dentine matrix protein 1 for biomimetic mineralization of dentine
Authors
Issue Date2013
Citation
Clinical Oral Investigations, 2014, v. 18 n. 3, p. 873-881 How to Cite?
AbstractObjective The objectives were to design and fabricate an oligopeptide that simulates dentine matrix protein 1 (DMP1) to study its ability to bind to dentine collagen fibrils and induce biomimetic mineralization for the management of dentine hypersensitivity. Materials and methods A novel oligopeptide was developed by connecting the collagen-binding domain of DMP1 to the hydrophilic C-terminal of amelogenin. Fluorescein isothiocyanate-coupled oligopeptide was applied to the completely demineralized dentine collagen and examined using fluorescent microscopy. The nucleation and growth of hydroxyapatite were initiated by immersing oligopeptide into calcium chloride and sodium hydrogen phosphate solutions. Scanning electron microscopy (SEM), transmission electron microscopy, and selected area electron diffraction (SAED) were used to examine the formation. Dentine slices were acid-etched, coated with oligopeptide, and immersed into a metastable calcium phosphate solution. Dentine mineralization was evaluated by SEM, X-ray diffraction (XRD), and Fourier transform infrared spectroscopy (FTIR). Results Fluorescent dentine collagen was identified in the specimens. The nucleation and growth of crystals were detected after immersing the oligopeptide into calcium chloride and sodium hydrogen phosphate solutions. Under SEM, crystals were observed covering the oligopeptide-coated dentine surface, within the demineralized dentine collagen matrix and occluding dentinal tubules. SAED, XRD, and FTIR confirmed that the crystals were hydroxyapatite. Conclusion A novel oligopeptide-simulating DMP1 was developed, that can bind to collagen fibrils, initiate mineralization, and induce biomimetic mineralization of dentine. Clinical relevance Biomimetic mineralization of dentine facilitated by this oligopeptide is a potential therapeutic technique for the management of dentine hypersensitivity.
Persistent Identifierhttp://hdl.handle.net/10722/192319
ISSN
2015 Impact Factor: 2.207
2015 SCImago Journal Rankings: 0.752
ISI Accession Number ID

 

DC FieldValueLanguage
dc.contributor.authorCao, Yen_US
dc.contributor.authorLiu, Wen_US
dc.contributor.authorNing, Ten_US
dc.contributor.authorMei, MLen_US
dc.contributor.authorLi, Q-Len_US
dc.contributor.authorLo, ECMen_US
dc.contributor.authorChu, CHen_US
dc.date.accessioned2013-10-24T01:49:29Z-
dc.date.available2013-10-24T01:49:29Z-
dc.date.issued2013en_US
dc.identifier.citationClinical Oral Investigations, 2014, v. 18 n. 3, p. 873-881en_US
dc.identifier.issn1432-6981en_US
dc.identifier.urihttp://hdl.handle.net/10722/192319-
dc.description.abstractObjective The objectives were to design and fabricate an oligopeptide that simulates dentine matrix protein 1 (DMP1) to study its ability to bind to dentine collagen fibrils and induce biomimetic mineralization for the management of dentine hypersensitivity. Materials and methods A novel oligopeptide was developed by connecting the collagen-binding domain of DMP1 to the hydrophilic C-terminal of amelogenin. Fluorescein isothiocyanate-coupled oligopeptide was applied to the completely demineralized dentine collagen and examined using fluorescent microscopy. The nucleation and growth of hydroxyapatite were initiated by immersing oligopeptide into calcium chloride and sodium hydrogen phosphate solutions. Scanning electron microscopy (SEM), transmission electron microscopy, and selected area electron diffraction (SAED) were used to examine the formation. Dentine slices were acid-etched, coated with oligopeptide, and immersed into a metastable calcium phosphate solution. Dentine mineralization was evaluated by SEM, X-ray diffraction (XRD), and Fourier transform infrared spectroscopy (FTIR). Results Fluorescent dentine collagen was identified in the specimens. The nucleation and growth of crystals were detected after immersing the oligopeptide into calcium chloride and sodium hydrogen phosphate solutions. Under SEM, crystals were observed covering the oligopeptide-coated dentine surface, within the demineralized dentine collagen matrix and occluding dentinal tubules. SAED, XRD, and FTIR confirmed that the crystals were hydroxyapatite. Conclusion A novel oligopeptide-simulating DMP1 was developed, that can bind to collagen fibrils, initiate mineralization, and induce biomimetic mineralization of dentine. Clinical relevance Biomimetic mineralization of dentine facilitated by this oligopeptide is a potential therapeutic technique for the management of dentine hypersensitivity.-
dc.languageengen_US
dc.relation.ispartofClinical Oral Investigationsen_US
dc.titleA novel oligopeptide simulating dentine matrix protein 1 for biomimetic mineralization of dentineen_US
dc.typeArticleen_US
dc.identifier.doi10.1007/s00784-013-1035-yen_US
dc.identifier.pmid23912147-
dc.identifier.scopuseid_2-s2.0-84897574563en_US
dc.identifier.hkuros226787-
dc.identifier.hkuros228337-
dc.identifier.hkuros238337-
dc.identifier.hkuros231334-
dc.identifier.spage873en_US
dc.identifier.epage881en_US
dc.identifier.isiWOS:000333799600023-

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