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Conference Paper: The diversity of Porcine Reproductive and Respiratory Syndrome virus type 1 and 2 in Denmark

TitleThe diversity of Porcine Reproductive and Respiratory Syndrome virus type 1 and 2 in Denmark
Authors
Issue Date2013
PublisherInternational PRRS Symposium.
Citation
The 2013 International PRRS Symposium and PCVAD (PCV2), Beijing, China, 20-22 May 2013. In Final Programme, 2013, p. 130, abstract no. 101 How to Cite?
AbstractBoth Type 1 and Type 2 PRRS viruses are circulating among Danish pigs. The first appearance of Type 1 PRRSV in Denmark was in 1992 whereas the Type 2 PRRSV was introduced in 1996 after the use of a live attenuated vaccine that reverted to virulence. Since then, vaccination to control the disease for both PRRSV genotypes has been widely used in Denmark and it is therefore highly relevant to monitor the diversity of currently circulating PRRSV strains. Only subtype 1 of the Type 1 PRRSV strains and vaccine-like Type 2 PRRSV strains were previously detected in Denmark, however, only few Danish PRRSV strains were sequenced. Denmark exports more than 50.000 living pigs each month. A portion of these pigs inevitably harbor PRRSV. Thus, the diversity of PRRSV in Denmark is of interest to other countries besides Denmark. The main objective of the present study was to close the gap in knowledge on the genetic diversity of currently circulating PRRSV stains in Danish pigs by sequencing ORF5 and ORF7 of approximately 41 Type 1 and 50 Type 2 strains isolated between 2003 and 2013. Furthermore, full genome analysis was performed on nine Type 1 and nine Type 2 selected strains. The preliminary assessment of the results showed that the Type 1 strains all belonged to subtype 1. Based on the ORF5 sequences, the Danish Type 1 viruses clustered into two groups. These two groups shared 84 % to 92 % and 94 % to 99 % nucleotide identity to the Lelystad virus, respectively. The sequenced Type 2 viruses showed a significant higher level of identity in that the ORF5 sequences were 94 - >99 % identical at the nucleotide level. Most of the Type 2 viruses, shared high level of identity to the VR2332 vaccine strain (Ingelvac MLV), but a few more diverse isolates were also identified, including strains with interesting deletions in NSP2 and other genes. The full genome sequences of Danish strains showed an overall nucleotide identity of 88-98 % (Type 1) and 94 % to >99 % (Type 2). The impact of these results will be discussed.
DescriptionSession - Viral Heterogeneity and Evolution
Persistent Identifierhttp://hdl.handle.net/10722/190673

 

DC FieldValueLanguage
dc.contributor.authorKvisgaard, LKen_US
dc.contributor.authorHjulsager, CKen_US
dc.contributor.authorKristensen, CSen_US
dc.contributor.authorBrar, MSen_US
dc.contributor.authorLeung, FCCen_US
dc.contributor.authorLarsen, LEen_US
dc.date.accessioned2013-09-17T15:34:49Z-
dc.date.available2013-09-17T15:34:49Z-
dc.date.issued2013en_US
dc.identifier.citationThe 2013 International PRRS Symposium and PCVAD (PCV2), Beijing, China, 20-22 May 2013. In Final Programme, 2013, p. 130, abstract no. 101en_US
dc.identifier.urihttp://hdl.handle.net/10722/190673-
dc.descriptionSession - Viral Heterogeneity and Evolution-
dc.description.abstractBoth Type 1 and Type 2 PRRS viruses are circulating among Danish pigs. The first appearance of Type 1 PRRSV in Denmark was in 1992 whereas the Type 2 PRRSV was introduced in 1996 after the use of a live attenuated vaccine that reverted to virulence. Since then, vaccination to control the disease for both PRRSV genotypes has been widely used in Denmark and it is therefore highly relevant to monitor the diversity of currently circulating PRRSV strains. Only subtype 1 of the Type 1 PRRSV strains and vaccine-like Type 2 PRRSV strains were previously detected in Denmark, however, only few Danish PRRSV strains were sequenced. Denmark exports more than 50.000 living pigs each month. A portion of these pigs inevitably harbor PRRSV. Thus, the diversity of PRRSV in Denmark is of interest to other countries besides Denmark. The main objective of the present study was to close the gap in knowledge on the genetic diversity of currently circulating PRRSV stains in Danish pigs by sequencing ORF5 and ORF7 of approximately 41 Type 1 and 50 Type 2 strains isolated between 2003 and 2013. Furthermore, full genome analysis was performed on nine Type 1 and nine Type 2 selected strains. The preliminary assessment of the results showed that the Type 1 strains all belonged to subtype 1. Based on the ORF5 sequences, the Danish Type 1 viruses clustered into two groups. These two groups shared 84 % to 92 % and 94 % to 99 % nucleotide identity to the Lelystad virus, respectively. The sequenced Type 2 viruses showed a significant higher level of identity in that the ORF5 sequences were 94 - >99 % identical at the nucleotide level. Most of the Type 2 viruses, shared high level of identity to the VR2332 vaccine strain (Ingelvac MLV), but a few more diverse isolates were also identified, including strains with interesting deletions in NSP2 and other genes. The full genome sequences of Danish strains showed an overall nucleotide identity of 88-98 % (Type 1) and 94 % to >99 % (Type 2). The impact of these results will be discussed.-
dc.languageengen_US
dc.publisherInternational PRRS Symposium.-
dc.relation.ispartofInternational PRRS Symposium and PCVAD (PCV2) 2013 Final Programmeen_US
dc.rightsCreative Commons: Attribution 3.0 Hong Kong License-
dc.titleThe diversity of Porcine Reproductive and Respiratory Syndrome virus type 1 and 2 in Denmarken_US
dc.typeConference_Paperen_US
dc.identifier.emailLeung, FCC: fcleung@hkucc.hku.hken_US
dc.identifier.authorityLeung, FCC=rp00731en_US
dc.description.naturepostprint-
dc.identifier.hkuros225006en_US
dc.identifier.spage130en_US
dc.identifier.epage130en_US
dc.publisher.placeChina-

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