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Conference Paper: Silver nanoparticles boost collagen maturation in Achilles tendon regeneration

TitleSilver nanoparticles boost collagen maturation in Achilles tendon regeneration
Authors
Issue Date2013
PublisherASME. The Conference abstracts' website is located at http://www.asmeconferences.org/NEMB2013/ViewAcceptedAbstracts.cfm
Citation
The ASME 2nd Global Congress on NanoEngineering for Medicine and Biology (NEMB 2013), Boston, MA., 4-6 February 2013. How to Cite?
AbstractBACKGROUND: Rupture Achilles tendon remains a problem in modern medicine. Although the content of the healed tendon should approximate the normal tendon after remodeling, the diameters and cross-linking of the collagen fibrils often remain inferior and are susceptible to tendon creep. The mechanical strength of the healed tendon is dictated by the quality of collagen fibrils and their spatial arrangement produced by the tenocytes during the healing process. Silver nanoparticles (AgNPs) has been shown to express antimicrobial effect, accelerate burn wound healing, reduce wound inflammation, modulate collagen deposition and encourage fibroblast differentiation in skin wound healing. Since both skin and tendon healing share similar pathways, it is hypothesized that AgNPs would enhance the healing of injured tendon. The objective of this study is to 1) investigate the in vitro response of tenocytes to AgNPs in the production of collagen; and 2) study the effect of AgNPs in the regeneration of rat Achilles tendon in vivo by assessing the mechanical strength and collagen maturity. MATERIALS AND METHODS: Primary tenocytes were harvested from the Achilles tendon of 4 weeks-old rats and cultured with AgNPs at different concentration. The collagen content was determined using Sirius red/ fast green staining on day 7 and 14. The collagen to non-collagen ratio was then calculated. In vivo rat Achilles tendon injury model was used to investigate the effect of AgNPs to tendon regeneration. Briefly, the Achilles tendon was transected and was either treated with local AgNPs injection every 5 days or left untreated as control. Skin incision was done without transecting the tendon in the sham group. The tendons were harvested at week 6. Tensile test was performed. The collagen fibrils maturity were assessed by Sirius red staining under the polarized microscope. RESULTS: From the results of the Sirius red/ fast green staining, AgNPs significantly increased collagen production at 10uM and 20uM. Tensile test results showed that the modulus of the AgNPs-treated samples significantly outweighed that of the control (p<0.05) and resembled the modulus of the sham. When viewed under polarized microscope using Sirius Red stain, the collagen fibres were found to be more mature in AgNPs-treated group than the control. DISCUSSION AND CONCLUSION: Collagen fibres constitute the tensile strength of tendon. AgNPs enhanced collagen production (in-vitro), encouraged collagen maturation, and improved tensile property in the regenerated tendon. To conclude, AgNPs remarkably enhance the healing of rupture Achilles tendon.
DescriptionTrack 3: Nanoengineering for Regenerative Medicine and Tissue Enginee... - Session: 3-4 Design and Characterization of Biomaterials: paper no. NEMB2013-93196
Persistent Identifierhttp://hdl.handle.net/10722/189958

 

DC FieldValueLanguage
dc.contributor.authorTo, Men_US
dc.contributor.authorKwan, KHLen_US
dc.contributor.authorWu, Jen_US
dc.contributor.authorYeung, Ken_US
dc.date.accessioned2013-09-17T15:04:12Z-
dc.date.available2013-09-17T15:04:12Z-
dc.date.issued2013en_US
dc.identifier.citationThe ASME 2nd Global Congress on NanoEngineering for Medicine and Biology (NEMB 2013), Boston, MA., 4-6 February 2013.en_US
dc.identifier.urihttp://hdl.handle.net/10722/189958-
dc.descriptionTrack 3: Nanoengineering for Regenerative Medicine and Tissue Enginee... - Session: 3-4 Design and Characterization of Biomaterials: paper no. NEMB2013-93196-
dc.description.abstractBACKGROUND: Rupture Achilles tendon remains a problem in modern medicine. Although the content of the healed tendon should approximate the normal tendon after remodeling, the diameters and cross-linking of the collagen fibrils often remain inferior and are susceptible to tendon creep. The mechanical strength of the healed tendon is dictated by the quality of collagen fibrils and their spatial arrangement produced by the tenocytes during the healing process. Silver nanoparticles (AgNPs) has been shown to express antimicrobial effect, accelerate burn wound healing, reduce wound inflammation, modulate collagen deposition and encourage fibroblast differentiation in skin wound healing. Since both skin and tendon healing share similar pathways, it is hypothesized that AgNPs would enhance the healing of injured tendon. The objective of this study is to 1) investigate the in vitro response of tenocytes to AgNPs in the production of collagen; and 2) study the effect of AgNPs in the regeneration of rat Achilles tendon in vivo by assessing the mechanical strength and collagen maturity. MATERIALS AND METHODS: Primary tenocytes were harvested from the Achilles tendon of 4 weeks-old rats and cultured with AgNPs at different concentration. The collagen content was determined using Sirius red/ fast green staining on day 7 and 14. The collagen to non-collagen ratio was then calculated. In vivo rat Achilles tendon injury model was used to investigate the effect of AgNPs to tendon regeneration. Briefly, the Achilles tendon was transected and was either treated with local AgNPs injection every 5 days or left untreated as control. Skin incision was done without transecting the tendon in the sham group. The tendons were harvested at week 6. Tensile test was performed. The collagen fibrils maturity were assessed by Sirius red staining under the polarized microscope. RESULTS: From the results of the Sirius red/ fast green staining, AgNPs significantly increased collagen production at 10uM and 20uM. Tensile test results showed that the modulus of the AgNPs-treated samples significantly outweighed that of the control (p<0.05) and resembled the modulus of the sham. When viewed under polarized microscope using Sirius Red stain, the collagen fibres were found to be more mature in AgNPs-treated group than the control. DISCUSSION AND CONCLUSION: Collagen fibres constitute the tensile strength of tendon. AgNPs enhanced collagen production (in-vitro), encouraged collagen maturation, and improved tensile property in the regenerated tendon. To conclude, AgNPs remarkably enhance the healing of rupture Achilles tendon.-
dc.languageengen_US
dc.publisherASME. The Conference abstracts' website is located at http://www.asmeconferences.org/NEMB2013/ViewAcceptedAbstracts.cfm-
dc.relation.ispartofASME 2nd Global Congress on Nanoengineering for Medicine and Biology, NEMB 2013en_US
dc.titleSilver nanoparticles boost collagen maturation in Achilles tendon regenerationen_US
dc.typeConference_Paperen_US
dc.identifier.emailTo, M: mikektto@hku.hken_US
dc.identifier.emailYeung, K: wkkyeung@hku.hken_US
dc.identifier.authorityTo, M=rp00302en_US
dc.identifier.authorityYeung, K=rp00309en_US
dc.description.naturelink_to_OA_fulltext-
dc.identifier.hkuros221252en_US
dc.publisher.placeUnited States-

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