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Conference Paper: Intrafibillarly-silicified Collagen Scaffolds for Tissue-Engineering. I. In-vitro Bioactivity, Osteogenic/Angiogenic Potential

TitleIntrafibillarly-silicified Collagen Scaffolds for Tissue-Engineering. I. In-vitro Bioactivity, Osteogenic/Angiogenic Potential
Authors
KeywordsBiocompatibility
Biomaterials
Bone repair
Regeneration and Tissue engineering
Issue Date2012
PublisherSage Publications, Inc.. The Journal's web site is located at http://www.sagepub.com/journalsProdDesc.nav?prodId=Journal201925
Citation
The 90th General Session & Exhibition of the International Association for Dental Research (IADR), Iguacu Falls, Brazil, 20-23 June 2012. In Journal of Dental Research, 2012, v. 91 n. Special issue B: abstract no. 196 How to Cite?
AbstractObjective: Highly-porous, biomimetic, intrafibrillarly-silicified collagen scaffolds (SCSs), inspired by diatom biosilicification, have been developed (Niu et al. Angew Chem Int Ed 2011;50:11688–91). This study examined the in-vitro bioactivity of SCSs, their biocompatibility and osteogenic/angiogenic potentials after exposure to murine mesenchymal stem cells (MSCs) and endothelial progenitor cells (EPCs). Method: SCS were prepared using choline-stabilized silicic acid to infiltrate poly(allylamine)-enriched, type I collagen sponges. Their in-vitro bioactivity, after immersion in simulated body fluid, was examined by TEM, selected-area electron diffraction and STEM-EDX. Viability/proliferation of murine MSCs and EPCs, after exposure to SCSs, was examined using MTT assay and flow cytometry. mRNA expressions of genes associated with osteogenesis and angiogenesis were examined with qRT-PCR. In-vitro osteogenesis of osteoblast-like cells differentiated from MSCs was examined by determining the calcium content of extracellular mineralization (Alizarin red S assay). In-vitro pro-angiogenesis was examined after culturing differentiated EPCs in ECMatrixTM and counting endothelial tube branch-points. Statistical analysis was performed for each parameter using one-way ANOVA and Holm-Sidak tests (α=0.05). Result: Apatite was deposited along the SCS surface after 48 h. SCSs were as biocompatible as non-silicified CSs control (MTT-succinic dehydrogenase activity; flow cytometry-% apoptotic cells; P>0.05). Differentiated MSCs exposed to SCSs exhibit highly-significant (P<0.001) fold-increases in expressions of genes associated with alkaline phosphatase, Runt-related transcription factor 2, osteocalcin, and bone sialoprotein II, compared with those without SCS exposure, or exposed to non-silicified CSs. Differentiated EPCs exposed to SCSs exhibit highly-significant (P<0.001) fold increases in expressions of vascular endothelial growth factor-A and angiopoetin-2 genes. The results of mRNA expressions were further confirmed by significantly more extracellular calcium deposits by differentiated MSCs, and tube formation by differentiated EPCs in the presence of SCSs, compared with non-silicified CS (P<0.05). Conclusion: The highly-desirable qualities of SCSs justify their loading with chemoattractants for stem/progenitor cell homing in hard tissue engineering.
DescriptionOral Presentation
Session 50: Bacteria: Biocompatibility and Biologic Effects
Persistent Identifierhttp://hdl.handle.net/10722/189687
ISSN
2015 Impact Factor: 4.602
2015 SCImago Journal Rankings: 1.714

 

DC FieldValueLanguage
dc.contributor.authorQi, Yen_US
dc.contributor.authorNiu, Len_US
dc.contributor.authorYiu, CKYen_US
dc.contributor.authorMesser, Ren_US
dc.contributor.authorKimmering, Ken_US
dc.contributor.authorPashley, Den_US
dc.contributor.authorTay, FRCMen_US
dc.date.accessioned2013-09-17T14:52:53Z-
dc.date.available2013-09-17T14:52:53Z-
dc.date.issued2012en_US
dc.identifier.citationThe 90th General Session & Exhibition of the International Association for Dental Research (IADR), Iguacu Falls, Brazil, 20-23 June 2012. In Journal of Dental Research, 2012, v. 91 n. Special issue B: abstract no. 196en_US
dc.identifier.issn0022-0345-
dc.identifier.urihttp://hdl.handle.net/10722/189687-
dc.descriptionOral Presentation-
dc.descriptionSession 50: Bacteria: Biocompatibility and Biologic Effects-
dc.description.abstractObjective: Highly-porous, biomimetic, intrafibrillarly-silicified collagen scaffolds (SCSs), inspired by diatom biosilicification, have been developed (Niu et al. Angew Chem Int Ed 2011;50:11688–91). This study examined the in-vitro bioactivity of SCSs, their biocompatibility and osteogenic/angiogenic potentials after exposure to murine mesenchymal stem cells (MSCs) and endothelial progenitor cells (EPCs). Method: SCS were prepared using choline-stabilized silicic acid to infiltrate poly(allylamine)-enriched, type I collagen sponges. Their in-vitro bioactivity, after immersion in simulated body fluid, was examined by TEM, selected-area electron diffraction and STEM-EDX. Viability/proliferation of murine MSCs and EPCs, after exposure to SCSs, was examined using MTT assay and flow cytometry. mRNA expressions of genes associated with osteogenesis and angiogenesis were examined with qRT-PCR. In-vitro osteogenesis of osteoblast-like cells differentiated from MSCs was examined by determining the calcium content of extracellular mineralization (Alizarin red S assay). In-vitro pro-angiogenesis was examined after culturing differentiated EPCs in ECMatrixTM and counting endothelial tube branch-points. Statistical analysis was performed for each parameter using one-way ANOVA and Holm-Sidak tests (α=0.05). Result: Apatite was deposited along the SCS surface after 48 h. SCSs were as biocompatible as non-silicified CSs control (MTT-succinic dehydrogenase activity; flow cytometry-% apoptotic cells; P>0.05). Differentiated MSCs exposed to SCSs exhibit highly-significant (P<0.001) fold-increases in expressions of genes associated with alkaline phosphatase, Runt-related transcription factor 2, osteocalcin, and bone sialoprotein II, compared with those without SCS exposure, or exposed to non-silicified CSs. Differentiated EPCs exposed to SCSs exhibit highly-significant (P<0.001) fold increases in expressions of vascular endothelial growth factor-A and angiopoetin-2 genes. The results of mRNA expressions were further confirmed by significantly more extracellular calcium deposits by differentiated MSCs, and tube formation by differentiated EPCs in the presence of SCSs, compared with non-silicified CS (P<0.05). Conclusion: The highly-desirable qualities of SCSs justify their loading with chemoattractants for stem/progenitor cell homing in hard tissue engineering.-
dc.languageengen_US
dc.publisherSage Publications, Inc.. The Journal's web site is located at http://www.sagepub.com/journalsProdDesc.nav?prodId=Journal201925-
dc.relation.ispartofJournal of Dental Researchen_US
dc.rightsJournal of Dental Research. Copyright © Sage Publications, Inc..-
dc.subjectBiocompatibility-
dc.subjectBiomaterials-
dc.subjectBone repair-
dc.subjectRegeneration and Tissue engineering-
dc.titleIntrafibillarly-silicified Collagen Scaffolds for Tissue-Engineering. I. In-vitro Bioactivity, Osteogenic/Angiogenic Potentialen_US
dc.typeConference_Paperen_US
dc.identifier.emailYiu, CKY: ckyyiu@hkucc.hku.hken_US
dc.identifier.emailTay, FRCM: franktay@hkucc.hku.hken_US
dc.identifier.authorityYiu, CKY=rp00018en_US
dc.identifier.hkuros224590en_US
dc.identifier.volume91en_US
dc.identifier.issueSpecial issue B: abstract no. 196en_US
dc.publisher.placeUnited States-

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