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Article: Membrane perforation and recovery dynamics in microbubble-mediated sonoporation

TitleMembrane perforation and recovery dynamics in microbubble-mediated sonoporation
Authors
KeywordsMembrane perforation
Membrane recovery
Real-time confocal imaging
Sonoporation
Spatiotemporal dynamics
Issue Date2013
PublisherElsevier Inc. The Journal's web site is located at http://www.elsevier.com/locate/ultrasmedbio
Citation
Ultrasound in Medicine and Biology, 2013, v. 39 n. 12, p. 2393-2405 How to Cite?
AbstractTransient sonoporation can essentially be epitomized by two fundamental processes: acoustically induced membrane perforation and its subsequent resealing. To provide insight into these processes, this article presents a new series of direct evidence on the membrane-level dynamics during and after an episode of sonoporation. Our direct observations were obtained from anchored fetal fibroblasts whose membrane topography was imaged in situ using real-time confocal microscopy. To facilitate controlled sonoporation at the single-cell level, microbubbles that can passively adhere to the cell membrane were first introduced at a 1:1 cell-to-bubble ratio. Single-pulse ultrasound exposure (1-MHz frequency, 10-cycle pulse duration, 0.85-MPa peak negative pressure in situ) was then applied to trigger microbubble pulsation/collapse, which, in turn, instigated membrane perforation. With this protocol, five membrane-level phenomena were observed: (i) localized perforation of the cell membrane was synchronized with the instant of ultrasound pulsing; (ii) perforation sites with temporal peak area <30 μm(2) were resealed successfully; (iii) during recovery, a thickened pore rim emerged, and its temporal progression corresponded with the pore closure action; (iv) membrane resealing, if successful, would generally be completed within 1 min of the onset of sonoporation, and the resealing time constant was estimated to be below 20 s; (v) membrane resealing would fail for overly large pores (>100 μm(2)) or in the absence of extracellular calcium ions. These findings serve to underscore the spatiotemporal complexity of membrane-level dynamics in sonoporation.
Persistent Identifierhttp://hdl.handle.net/10722/189047
ISSN
2015 Impact Factor: 2.298
2015 SCImago Journal Rankings: 0.885

 

DC FieldValueLanguage
dc.contributor.authorHu, Y-
dc.contributor.authorWan, JMF-
dc.contributor.authorYu, ACH-
dc.date.accessioned2013-09-17T14:24:47Z-
dc.date.available2013-09-17T14:24:47Z-
dc.date.issued2013-
dc.identifier.citationUltrasound in Medicine and Biology, 2013, v. 39 n. 12, p. 2393-2405-
dc.identifier.issn0301-5629-
dc.identifier.urihttp://hdl.handle.net/10722/189047-
dc.description.abstractTransient sonoporation can essentially be epitomized by two fundamental processes: acoustically induced membrane perforation and its subsequent resealing. To provide insight into these processes, this article presents a new series of direct evidence on the membrane-level dynamics during and after an episode of sonoporation. Our direct observations were obtained from anchored fetal fibroblasts whose membrane topography was imaged in situ using real-time confocal microscopy. To facilitate controlled sonoporation at the single-cell level, microbubbles that can passively adhere to the cell membrane were first introduced at a 1:1 cell-to-bubble ratio. Single-pulse ultrasound exposure (1-MHz frequency, 10-cycle pulse duration, 0.85-MPa peak negative pressure in situ) was then applied to trigger microbubble pulsation/collapse, which, in turn, instigated membrane perforation. With this protocol, five membrane-level phenomena were observed: (i) localized perforation of the cell membrane was synchronized with the instant of ultrasound pulsing; (ii) perforation sites with temporal peak area <30 μm(2) were resealed successfully; (iii) during recovery, a thickened pore rim emerged, and its temporal progression corresponded with the pore closure action; (iv) membrane resealing, if successful, would generally be completed within 1 min of the onset of sonoporation, and the resealing time constant was estimated to be below 20 s; (v) membrane resealing would fail for overly large pores (>100 μm(2)) or in the absence of extracellular calcium ions. These findings serve to underscore the spatiotemporal complexity of membrane-level dynamics in sonoporation.-
dc.languageeng-
dc.publisherElsevier Inc. The Journal's web site is located at http://www.elsevier.com/locate/ultrasmedbio-
dc.relation.ispartofUltrasound in Medicine and Biology-
dc.subjectMembrane perforation-
dc.subjectMembrane recovery-
dc.subjectReal-time confocal imaging-
dc.subjectSonoporation-
dc.subjectSpatiotemporal dynamics-
dc.titleMembrane perforation and recovery dynamics in microbubble-mediated sonoporation-
dc.typeArticle-
dc.identifier.emailWan, JMF: jmfwan@hku.hk-
dc.identifier.emailYu, ACH: alfred.yu@hku.hk-
dc.identifier.authorityWan, JMF=rp00798-
dc.identifier.authorityYu, ACH=rp00657-
dc.identifier.doi10.1016/j.ultrasmedbio.2013.08.003-
dc.identifier.pmid24063956-
dc.identifier.hkuros222132-
dc.identifier.volume39-
dc.identifier.issue12-
dc.identifier.spage2393-
dc.identifier.epage2405-
dc.publisher.placeUnited States-

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