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Article: Topical treatment with Tong-Luo-San-Jie gel alleviates bone cancer pain in rats

TitleTopical treatment with Tong-Luo-San-Jie gel alleviates bone cancer pain in rats
Authors
Issue Date2012
PublisherElsevier Ireland Ltd. The Journal's web site is located at http://www.elsevier.com/locate/jethpharm
Citation
Journal Of Ethnopharmacology, 2012, v. 143 n. 3, p. 905-913 How to Cite?
AbstractEthnopharmacological relevance: The herbal analgesic gel Tong-Luo-San-Jie (TLSJ) and its modifications are used in traditional Chinese medicine to manage cancer pain. However, its mechanisms are still unknown. Aim of the study: To investigate the effects and mechanisms of TLSJ gel on bone cancer pain in a rat model. Materials and methods: A bone cancer pain rat model was established by inoculating Walker 256 rat carcinoma cells directly into the right tibial medullary cavity of Sprague-Dawley rats (150-170 g); Phosphate buffered saline (PBS) tibial inoculation was used as control. Cancer-bearing rats were treated twice a day with external TLSJ gel (0.5 g/cm2/day) or inert gel control for 21 day (n=10/group). Behavioral tests such as mechanical threshold and paw withdrawal latency (PWL) were carried out. Osteoclastic activities were determined and carboxyterminal pyridinoline cross-linked type I collagen telopeptides (ICTP) and bone-specific alkaline phosphatase (BAP) concentrations were detected with ELISA after treatment. Adverse effects were monitored, and biochemical and histological tests were performed in naïve rats treated with local TLSJ gel for six weeks. Results: TLSJ treatment significantly restored bone cancer-induced decrease of PWL and mechanical threshold compared to inert gel. It also decreased the level of blood serum ICTP and BAP and inhibited osteoclast activities. No adverse effects or abnormal biochemical and histological changes were detected after TLSJ treatment. Conclusion: The present study shows that TLSJ significantly inhibits bone cancer-induced thermal and mechanical sensitization. It suggests that the gel may be useful in managing cancer pain and that it may act by inhibiting osteoclastic activity. © 2012 Elsevier Ireland Ltd. All rights reserved.
Persistent Identifierhttp://hdl.handle.net/10722/188656
ISSN
2015 Impact Factor: 3.055
2015 SCImago Journal Rankings: 1.156
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorWang, Jen_US
dc.contributor.authorZhang, Ren_US
dc.contributor.authorDong, Cen_US
dc.contributor.authorJiao, Len_US
dc.contributor.authorXu, Len_US
dc.contributor.authorLiu, Jen_US
dc.contributor.authorWang, Zen_US
dc.contributor.authorMao Ying, QLen_US
dc.contributor.authorFong, Hen_US
dc.contributor.authorLao, Len_US
dc.date.accessioned2013-09-03T04:10:53Z-
dc.date.available2013-09-03T04:10:53Z-
dc.date.issued2012en_US
dc.identifier.citationJournal Of Ethnopharmacology, 2012, v. 143 n. 3, p. 905-913en_US
dc.identifier.issn0378-8741en_US
dc.identifier.urihttp://hdl.handle.net/10722/188656-
dc.description.abstractEthnopharmacological relevance: The herbal analgesic gel Tong-Luo-San-Jie (TLSJ) and its modifications are used in traditional Chinese medicine to manage cancer pain. However, its mechanisms are still unknown. Aim of the study: To investigate the effects and mechanisms of TLSJ gel on bone cancer pain in a rat model. Materials and methods: A bone cancer pain rat model was established by inoculating Walker 256 rat carcinoma cells directly into the right tibial medullary cavity of Sprague-Dawley rats (150-170 g); Phosphate buffered saline (PBS) tibial inoculation was used as control. Cancer-bearing rats were treated twice a day with external TLSJ gel (0.5 g/cm2/day) or inert gel control for 21 day (n=10/group). Behavioral tests such as mechanical threshold and paw withdrawal latency (PWL) were carried out. Osteoclastic activities were determined and carboxyterminal pyridinoline cross-linked type I collagen telopeptides (ICTP) and bone-specific alkaline phosphatase (BAP) concentrations were detected with ELISA after treatment. Adverse effects were monitored, and biochemical and histological tests were performed in naïve rats treated with local TLSJ gel for six weeks. Results: TLSJ treatment significantly restored bone cancer-induced decrease of PWL and mechanical threshold compared to inert gel. It also decreased the level of blood serum ICTP and BAP and inhibited osteoclast activities. No adverse effects or abnormal biochemical and histological changes were detected after TLSJ treatment. Conclusion: The present study shows that TLSJ significantly inhibits bone cancer-induced thermal and mechanical sensitization. It suggests that the gel may be useful in managing cancer pain and that it may act by inhibiting osteoclastic activity. © 2012 Elsevier Ireland Ltd. All rights reserved.en_US
dc.languageengen_US
dc.publisherElsevier Ireland Ltd. The Journal's web site is located at http://www.elsevier.com/locate/jethpharmen_US
dc.relation.ispartofJournal of Ethnopharmacologyen_US
dc.subject.meshAlkaline Phosphatase - Blooden_US
dc.subject.meshAnalgesics - Administration & Dosageen_US
dc.subject.meshAnimalsen_US
dc.subject.meshBone Neoplasms - Complications - Drug Therapyen_US
dc.subject.meshCarcinoma 256, Walker - Complications - Drug Therapyen_US
dc.subject.meshCollagen Type I - Blooden_US
dc.subject.meshDrugs, Chinese Herbal - Administration & Dosageen_US
dc.subject.meshFemaleen_US
dc.subject.meshGelsen_US
dc.subject.meshMaleen_US
dc.subject.meshOsteoclasts - Drug Effectsen_US
dc.subject.meshPain - Drug Therapy - Etiologyen_US
dc.subject.meshPeptides - Blooden_US
dc.subject.meshPhytotherapyen_US
dc.subject.meshRatsen_US
dc.subject.meshRats, Sprague-Dawleyen_US
dc.titleTopical treatment with Tong-Luo-San-Jie gel alleviates bone cancer pain in ratsen_US
dc.typeArticleen_US
dc.identifier.emailLao, L: lxlao1@hku.hken_US
dc.identifier.authorityLao, L=rp01784en_US
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.doi10.1016/j.jep.2012.08.026en_US
dc.identifier.pmid22960543-
dc.identifier.scopuseid_2-s2.0-84866731652en_US
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-84866731652&selection=ref&src=s&origin=recordpageen_US
dc.identifier.volume143en_US
dc.identifier.issue3en_US
dc.identifier.spage905en_US
dc.identifier.epage913en_US
dc.identifier.isiWOS:000309990000018-
dc.publisher.placeIrelanden_US
dc.identifier.scopusauthoridWang, J=11739667100en_US
dc.identifier.scopusauthoridZhang, R=7404864527en_US
dc.identifier.scopusauthoridDong, C=55233377100en_US
dc.identifier.scopusauthoridJiao, L=55350607300en_US
dc.identifier.scopusauthoridXu, L=36524242100en_US
dc.identifier.scopusauthoridLiu, J=54684437300en_US
dc.identifier.scopusauthoridWang, Z=8312649700en_US
dc.identifier.scopusauthoridMao Ying, QL=13806075200en_US
dc.identifier.scopusauthoridFong, H=55351534100en_US
dc.identifier.scopusauthoridLao, L=7005681883en_US

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