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Article: Role of neuronal nitric oxide synthase in colonic distension-induced hyperalgesia in distal colon of neonatal maternal separated male rats

TitleRole of neuronal nitric oxide synthase in colonic distension-induced hyperalgesia in distal colon of neonatal maternal separated male rats
Authors
Issue Date2011
PublisherWiley-Blackwell. The Journal's web site is located at http://www.wiley.com/bw/journal.asp?ref=1350-1925&site=1
Citation
Neurogastroenterology And Motility, 2011, v. 23 n. 7, p. 666-e278 How to Cite?
AbstractBackground Nitric oxide (NO) is implicated in the pathogenesis of irritable bowel syndrome (IBS) but the underlying mechanism is unclear. Thus, the aim of the present study is to examine the role of NO synthase (NOS) expression in the distal colon of neonatal maternal separation (NMS) model rats employed in IBS studies. Methods Male neonates of Sprague-Dawley rats were randomly assigned into NMS and normal control (N) groups. Rats of NMS group were subjected to 3h daily maternal separation on postnatal day 2-21. Rats were administrated non-selective NOS inhibitor l-NAME (100mgkg -1), selective neuronal NOS (nNOS) inhibitor 7-NINA (10mgkg -1), selective inducible NOS (iNOS) inhibitor, endothelial NOS (eNOS) inhibitor (10mgkg -1) or Vehicle (Veh; distilled water) intraperitoneally 1h prior to the experiment for the test and control groups, respectively. Key Results The amount of NO was significantly higher in the NMS Veh rats compared with unseparated N rats. Western-blotting and real-time quantitative PCR studies showed that protein and mRNA expression of nNOS were higher in the NMS group than that in the N rats; whereas no significant change in iNOS and eNOS was found in either groups. Neonatal maternal separation Veh rats showed low pain threshold and increased electromyogram (EMG) activity in response to colonic distension stimuli. l-NAME and 7-Nitroindazole monosodium salt (7-NINA) increased pain threshold pressure and attenuated EMG activity in the NMS rats. In addition, l-NAME and 7-NINA substantially reduced oxidative marker malondialdehyde level in NMS rats. Conclusions & Inferences Neonatal maternal separation increased the NO generation by nNOS upregulation that interact with reactive oxygen species contributing to the visceral hypersensitivity in IBS. © 2011 Blackwell Publishing Ltd.
Persistent Identifierhttp://hdl.handle.net/10722/188631
ISSN
2015 Impact Factor: 3.31
2015 SCImago Journal Rankings: 1.722
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorTjong, YWen_US
dc.contributor.authorIp, SPen_US
dc.contributor.authorLao, Len_US
dc.contributor.authorWu, Jen_US
dc.contributor.authorFong, HHSen_US
dc.contributor.authorSung, JJYen_US
dc.contributor.authorBerman, Ben_US
dc.contributor.authorChe, CTen_US
dc.date.accessioned2013-09-03T04:10:43Z-
dc.date.available2013-09-03T04:10:43Z-
dc.date.issued2011en_US
dc.identifier.citationNeurogastroenterology And Motility, 2011, v. 23 n. 7, p. 666-e278en_US
dc.identifier.issn1350-1925en_US
dc.identifier.urihttp://hdl.handle.net/10722/188631-
dc.description.abstractBackground Nitric oxide (NO) is implicated in the pathogenesis of irritable bowel syndrome (IBS) but the underlying mechanism is unclear. Thus, the aim of the present study is to examine the role of NO synthase (NOS) expression in the distal colon of neonatal maternal separation (NMS) model rats employed in IBS studies. Methods Male neonates of Sprague-Dawley rats were randomly assigned into NMS and normal control (N) groups. Rats of NMS group were subjected to 3h daily maternal separation on postnatal day 2-21. Rats were administrated non-selective NOS inhibitor l-NAME (100mgkg -1), selective neuronal NOS (nNOS) inhibitor 7-NINA (10mgkg -1), selective inducible NOS (iNOS) inhibitor, endothelial NOS (eNOS) inhibitor (10mgkg -1) or Vehicle (Veh; distilled water) intraperitoneally 1h prior to the experiment for the test and control groups, respectively. Key Results The amount of NO was significantly higher in the NMS Veh rats compared with unseparated N rats. Western-blotting and real-time quantitative PCR studies showed that protein and mRNA expression of nNOS were higher in the NMS group than that in the N rats; whereas no significant change in iNOS and eNOS was found in either groups. Neonatal maternal separation Veh rats showed low pain threshold and increased electromyogram (EMG) activity in response to colonic distension stimuli. l-NAME and 7-Nitroindazole monosodium salt (7-NINA) increased pain threshold pressure and attenuated EMG activity in the NMS rats. In addition, l-NAME and 7-NINA substantially reduced oxidative marker malondialdehyde level in NMS rats. Conclusions & Inferences Neonatal maternal separation increased the NO generation by nNOS upregulation that interact with reactive oxygen species contributing to the visceral hypersensitivity in IBS. © 2011 Blackwell Publishing Ltd.en_US
dc.languageengen_US
dc.publisherWiley-Blackwell. The Journal's web site is located at http://www.wiley.com/bw/journal.asp?ref=1350-1925&site=1en_US
dc.relation.ispartofNeurogastroenterology and Motilityen_US
dc.subject.meshAnimalsen_US
dc.subject.meshAnimals, Newborn - Physiologyen_US
dc.subject.meshColon - Physiopathologyen_US
dc.subject.meshColonic Diseases - Physiopathologyen_US
dc.subject.meshDisease Models, Animalen_US
dc.subject.meshElectromyographyen_US
dc.subject.meshGastrointestinal Motility - Physiologyen_US
dc.subject.meshHyperalgesia - Physiopathologyen_US
dc.subject.meshIndazoles - Pharmacologyen_US
dc.subject.meshIrritable Bowel Syndrome - Physiopathologyen_US
dc.subject.meshMaleen_US
dc.subject.meshMaternal Behavior - Physiologyen_US
dc.subject.meshNg-Nitroarginine Methyl Ester - Pharmacologyen_US
dc.subject.meshNitric Oxide Synthase Type I - Antagonists & Inhibitors - Drug Effects - Physiologyen_US
dc.subject.meshNitric Oxide Synthase Type Ii - Antagonists & Inhibitors - Drug Effects - Physiologyen_US
dc.subject.meshNitric Oxide Synthase Type Iii - Antagonists & Inhibitors - Drug Effects - Physiologyen_US
dc.subject.meshOxidative Stress - Drug Effectsen_US
dc.subject.meshRatsen_US
dc.subject.meshRats, Sprague-Dawleyen_US
dc.subject.meshStress, Psychological - Physiopathologyen_US
dc.titleRole of neuronal nitric oxide synthase in colonic distension-induced hyperalgesia in distal colon of neonatal maternal separated male ratsen_US
dc.typeArticleen_US
dc.identifier.emailLao, L: lxlao1@hku.hken_US
dc.identifier.authorityLao, L=rp01784en_US
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.doi10.1111/j.1365-2982.2011.01697.xen_US
dc.identifier.pmid21410601-
dc.identifier.scopuseid_2-s2.0-79959305692en_US
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-79959305692&selection=ref&src=s&origin=recordpageen_US
dc.identifier.volume23en_US
dc.identifier.issue7en_US
dc.identifier.spage666en_US
dc.identifier.epagee278en_US
dc.identifier.isiWOS:000292833800017-
dc.publisher.placeUnited Kingdomen_US
dc.identifier.scopusauthoridTjong, YW=55405455300en_US
dc.identifier.scopusauthoridIp, SP=7006626448en_US
dc.identifier.scopusauthoridLao, L=7005681883en_US
dc.identifier.scopusauthoridWu, J=7409253910en_US
dc.identifier.scopusauthoridFong, HHS=34569199300en_US
dc.identifier.scopusauthoridSung, JJY=35405352400en_US
dc.identifier.scopusauthoridBerman, B=35458606800en_US
dc.identifier.scopusauthoridChe, CT=7102442768en_US

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