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Article: Analgesic effect of Coptis chinensis rhizomes (Coptidis Rhizoma) extract on rat model of irritable bowel syndrome

TitleAnalgesic effect of Coptis chinensis rhizomes (Coptidis Rhizoma) extract on rat model of irritable bowel syndrome
Authors
Issue Date2011
PublisherElsevier Ireland Ltd. The Journal's web site is located at http://www.elsevier.com/locate/jethpharm
Citation
Journal Of Ethnopharmacology, 2011, v. 135 n. 3, p. 754-761 How to Cite?
AbstractEthnopharmacological relevance: Coptis chinensis rhizomes (Coptidis Rhizoma, CR), also known as "Huang Lian", is a common component of traditional Chinese herbal formulae used for the relief of abdominal pain and diarrhea. Yet, the action mechanism of CR extract in the treatment of irritable bowel syndrome is unknown. Thus, the aim of our present study is to investigate the effect of CR extract on neonatal maternal separation (NMS)-induced visceral hyperalgesia in rats and its underlying action mechanisms. Materials and methods: Male Sprague-Dawley rats were subjected to 3-h daily maternal separation from postnatal day 2 to day 21 to form the NMS group. The control group consists of unseparated normal (N) rats. From day 60, rats were administrated CR (0.3, 0.8 and 1.3 g/kg) or vehicle (Veh; 0.5% carboxymethylcellulose solution) orally for 7 days for the test and control groups, respectively. Results: Electromyogram (EMG) signals in response to colonic distension were measured with the NMS rats showing lower pain threshold and increased EMG activity than those of the unseparated (N) rats. CR dose-dependently increased pain threshold response and attenuated EMG activity in the NMS rats. An enzymatic immunoassay study showed that CR treatment significantly reduced the serotonin (5HT) concentration from the distal colon of NMS rats compared to the Veh (control) group. Real-time quantitative PCR and Western-blotting studies showed that CR treatment substantially reduced NMS induced cholecystokinin (CCK) expression compared with the Veh group. Conclusions: These results suggest that CR extract robustly reduces visceral pain that may be mediated via the mechanism of decreasing 5HT release and CCK expression in the distal colon of rats. © 2011 Elsevier Ireland Ltd. All rights reserved.
Persistent Identifierhttp://hdl.handle.net/10722/188629
ISSN
2015 Impact Factor: 3.055
2015 SCImago Journal Rankings: 1.156
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorTjong, Yen_US
dc.contributor.authorIp, Sen_US
dc.contributor.authorLao, Len_US
dc.contributor.authorFong, HHSen_US
dc.contributor.authorSung, JJYen_US
dc.contributor.authorBerman, Ben_US
dc.contributor.authorChe, Cen_US
dc.date.accessioned2013-09-03T04:10:42Z-
dc.date.available2013-09-03T04:10:42Z-
dc.date.issued2011en_US
dc.identifier.citationJournal Of Ethnopharmacology, 2011, v. 135 n. 3, p. 754-761en_US
dc.identifier.issn0378-8741en_US
dc.identifier.urihttp://hdl.handle.net/10722/188629-
dc.description.abstractEthnopharmacological relevance: Coptis chinensis rhizomes (Coptidis Rhizoma, CR), also known as "Huang Lian", is a common component of traditional Chinese herbal formulae used for the relief of abdominal pain and diarrhea. Yet, the action mechanism of CR extract in the treatment of irritable bowel syndrome is unknown. Thus, the aim of our present study is to investigate the effect of CR extract on neonatal maternal separation (NMS)-induced visceral hyperalgesia in rats and its underlying action mechanisms. Materials and methods: Male Sprague-Dawley rats were subjected to 3-h daily maternal separation from postnatal day 2 to day 21 to form the NMS group. The control group consists of unseparated normal (N) rats. From day 60, rats were administrated CR (0.3, 0.8 and 1.3 g/kg) or vehicle (Veh; 0.5% carboxymethylcellulose solution) orally for 7 days for the test and control groups, respectively. Results: Electromyogram (EMG) signals in response to colonic distension were measured with the NMS rats showing lower pain threshold and increased EMG activity than those of the unseparated (N) rats. CR dose-dependently increased pain threshold response and attenuated EMG activity in the NMS rats. An enzymatic immunoassay study showed that CR treatment significantly reduced the serotonin (5HT) concentration from the distal colon of NMS rats compared to the Veh (control) group. Real-time quantitative PCR and Western-blotting studies showed that CR treatment substantially reduced NMS induced cholecystokinin (CCK) expression compared with the Veh group. Conclusions: These results suggest that CR extract robustly reduces visceral pain that may be mediated via the mechanism of decreasing 5HT release and CCK expression in the distal colon of rats. © 2011 Elsevier Ireland Ltd. All rights reserved.en_US
dc.languageengen_US
dc.publisherElsevier Ireland Ltd. The Journal's web site is located at http://www.elsevier.com/locate/jethpharmen_US
dc.relation.ispartofJournal of Ethnopharmacologyen_US
dc.subject.meshAbdominal Pain - Drug Therapy - Etiologyen_US
dc.subject.meshAnalgesics - Pharmacology - Therapeutic Useen_US
dc.subject.meshAnimalsen_US
dc.subject.meshCholecystokinin - Metabolismen_US
dc.subject.meshColon - Drug Effects - Metabolism - Pathologyen_US
dc.subject.meshCoptisen_US
dc.subject.meshDisease Models, Animalen_US
dc.subject.meshDose-Response Relationship, Drugen_US
dc.subject.meshDrugs, Chinese Herbal - Pharmacology - Therapeutic Useen_US
dc.subject.meshElectromyography - Methodsen_US
dc.subject.meshHyperalgesia - Drug Therapy - Etiologyen_US
dc.subject.meshIrritable Bowel Syndrome - Complications - Drug Therapyen_US
dc.subject.meshMaleen_US
dc.subject.meshMaternal Deprivationen_US
dc.subject.meshMuscle, Smooth - Drug Effects - Pathologyen_US
dc.subject.meshPain Threshold - Drug Effectsen_US
dc.subject.meshPhytotherapyen_US
dc.subject.meshRatsen_US
dc.subject.meshRats, Sprague-Dawleyen_US
dc.subject.meshRhizomeen_US
dc.subject.meshSerotonin - Metabolismen_US
dc.subject.meshStress, Psychologicalen_US
dc.titleAnalgesic effect of Coptis chinensis rhizomes (Coptidis Rhizoma) extract on rat model of irritable bowel syndromeen_US
dc.typeArticleen_US
dc.identifier.emailLao, L: lxlao1@hku.hken_US
dc.identifier.authorityLao, L=rp01784en_US
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.doi10.1016/j.jep.2011.04.007en_US
dc.identifier.pmid21511022-
dc.identifier.scopuseid_2-s2.0-79957526160en_US
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-79957526160&selection=ref&src=s&origin=recordpageen_US
dc.identifier.volume135en_US
dc.identifier.issue3en_US
dc.identifier.spage754en_US
dc.identifier.epage761en_US
dc.identifier.isiWOS:000291960100020-
dc.publisher.placeIrelanden_US
dc.identifier.scopusauthoridTjong, Y=55405455300en_US
dc.identifier.scopusauthoridIp, S=7006626448en_US
dc.identifier.scopusauthoridLao, L=7005681883en_US
dc.identifier.scopusauthoridFong, HHS=34569199300en_US
dc.identifier.scopusauthoridSung, JJY=35405352400en_US
dc.identifier.scopusauthoridBerman, B=35458606800en_US
dc.identifier.scopusauthoridChe, C=7102442768en_US

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