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Article: Neonatal maternal separation elevates thalamic corticotropin releasing factor type 1 receptor expression response to colonic distension in rat

TitleNeonatal maternal separation elevates thalamic corticotropin releasing factor type 1 receptor expression response to colonic distension in rat
Authors
Issue Date2010
PublisherMaghira & Maas Publications. The Journal's web site is located at http://www.nel.edu
Citation
Neuroendocrinology Letters, 2010, v. 31 n. 2, p. 215-220 How to Cite?
AbstractOBJECTIVES: Early life psychological stress is an essential factor contributing to the development of irritable bowel syndrome (IBS), with corticotrophin releasing factor (CRF) having been implicated in this common gastrointestinal disorder. The aim of our study is to examine the effect of neonatal maternal separation (NMS), an early life stress model, on the brain CRF expression following visceral pain induced by colorectal distension (CRD) stimuli in male rats. METHODS: Male neonatal Sprague-Dawley rats were subjected to 3-hr daily maternal separation on postnatal day 2-21, with unseparated normal (N) rats serving as controls. Electromyogram signals (EMG) in response to phasic CRD were measured. The results demonstrated an increased pain response and EMG magnitudes in NMS rats as compared to N rats in response to CRD stimulation. The mRNA and protein expressions of CRF in hippocampus, cortex and thalamus of NMS and N group following the CRD stress were determined by real-time quantitative PCR and western-blotting studies respectively. RESULTS: There was an increased mRNA and protein level of CRF in thalamus of NMS rats but no apparent change in CRF expression in hippocampus and cortex of both groups. Furthermore, an increased expression of CRF type 1 receptor (CRF-Rl) was observed in the thalamus of NMS rats. CONCLUSION: These results suggested an up-regulation of thalamus CRF-R1 is associated with visceral hyperalgesia in the rat model of NMS. © 2010 Neuroendocrinology Letters.
Persistent Identifierhttp://hdl.handle.net/10722/188619
ISSN
2015 Impact Factor: 0.946
2015 SCImago Journal Rankings: 0.383
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorTjong, YWen_US
dc.contributor.authorIp, SPen_US
dc.contributor.authorLao, Len_US
dc.contributor.authorWu, Jen_US
dc.contributor.authorFong, HHSen_US
dc.contributor.authorSung, JJYen_US
dc.contributor.authorBerman, Ben_US
dc.contributor.authorChe, CTen_US
dc.date.accessioned2013-09-03T04:10:39Z-
dc.date.available2013-09-03T04:10:39Z-
dc.date.issued2010en_US
dc.identifier.citationNeuroendocrinology Letters, 2010, v. 31 n. 2, p. 215-220en_US
dc.identifier.issn0172-780Xen_US
dc.identifier.urihttp://hdl.handle.net/10722/188619-
dc.description.abstractOBJECTIVES: Early life psychological stress is an essential factor contributing to the development of irritable bowel syndrome (IBS), with corticotrophin releasing factor (CRF) having been implicated in this common gastrointestinal disorder. The aim of our study is to examine the effect of neonatal maternal separation (NMS), an early life stress model, on the brain CRF expression following visceral pain induced by colorectal distension (CRD) stimuli in male rats. METHODS: Male neonatal Sprague-Dawley rats were subjected to 3-hr daily maternal separation on postnatal day 2-21, with unseparated normal (N) rats serving as controls. Electromyogram signals (EMG) in response to phasic CRD were measured. The results demonstrated an increased pain response and EMG magnitudes in NMS rats as compared to N rats in response to CRD stimulation. The mRNA and protein expressions of CRF in hippocampus, cortex and thalamus of NMS and N group following the CRD stress were determined by real-time quantitative PCR and western-blotting studies respectively. RESULTS: There was an increased mRNA and protein level of CRF in thalamus of NMS rats but no apparent change in CRF expression in hippocampus and cortex of both groups. Furthermore, an increased expression of CRF type 1 receptor (CRF-Rl) was observed in the thalamus of NMS rats. CONCLUSION: These results suggested an up-regulation of thalamus CRF-R1 is associated with visceral hyperalgesia in the rat model of NMS. © 2010 Neuroendocrinology Letters.en_US
dc.languageengen_US
dc.publisherMaghira & Maas Publications. The Journal's web site is located at http://www.nel.eduen_US
dc.relation.ispartofNeuroendocrinology Lettersen_US
dc.subject.meshAbdominal Pain - Metabolism - Physiopathology - Psychologyen_US
dc.subject.meshAnimalsen_US
dc.subject.meshAnimals, Newborn - Metabolism - Psychologyen_US
dc.subject.meshCerebral Cortex - Metabolismen_US
dc.subject.meshColon - Physiopathologyen_US
dc.subject.meshDilatation, Pathologic - Physiopathologyen_US
dc.subject.meshElectromyographyen_US
dc.subject.meshHippocampus - Metabolismen_US
dc.subject.meshHyperalgesia - Metabolism - Physiopathology - Psychologyen_US
dc.subject.meshMaleen_US
dc.subject.meshMaternal Deprivationen_US
dc.subject.meshPain Threshold - Psychologyen_US
dc.subject.meshRna, Messenger - Metabolismen_US
dc.subject.meshRatsen_US
dc.subject.meshRats, Sprague-Dawleyen_US
dc.subject.meshReceptors, Corticotropin-Releasing Hormone - Genetics - Metabolismen_US
dc.subject.meshRectum - Physiopathologyen_US
dc.subject.meshStress, Psychological - Metabolismen_US
dc.subject.meshThalamus - Metabolismen_US
dc.subject.meshUp-Regulation - Geneticsen_US
dc.titleNeonatal maternal separation elevates thalamic corticotropin releasing factor type 1 receptor expression response to colonic distension in raten_US
dc.typeArticleen_US
dc.identifier.emailLao, L: lxlao1@hku.hken_US
dc.identifier.authorityLao, L=rp01784en_US
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.pmid20424584-
dc.identifier.scopuseid_2-s2.0-77952403817en_US
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-77952403817&selection=ref&src=s&origin=recordpageen_US
dc.identifier.volume31en_US
dc.identifier.issue2en_US
dc.identifier.spage215en_US
dc.identifier.epage220en_US
dc.identifier.isiWOS:000277702100010-
dc.publisher.placeSwedenen_US
dc.identifier.scopusauthoridTjong, YW=55405455300en_US
dc.identifier.scopusauthoridIp, SP=7006626448en_US
dc.identifier.scopusauthoridLao, L=7005681883en_US
dc.identifier.scopusauthoridWu, J=7409253910en_US
dc.identifier.scopusauthoridFong, HHS=34569199300en_US
dc.identifier.scopusauthoridSung, JJY=35405352400en_US
dc.identifier.scopusauthoridBerman, B=35458606800en_US
dc.identifier.scopusauthoridChe, CT=7102442768en_US

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