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Article: Mechanisms of treatment of cancer pain with a topical Chinese herbal formula in rats

TitleMechanisms of treatment of cancer pain with a topical Chinese herbal formula in rats
Authors
Issue Date2009
PublisherChinese Medical Association. The Journal's web site is located at http://www.cmj.org/
Citation
Chinese Medical Journal, 2009, v. 122 n. 17, p. 2027-2031 How to Cite?
AbstractBackground: Pain has a substantial impact on patients' activities and overall quality of life, but current conventional drugs have debilitating side effects, including gastrointestinal disorders. Thus there is a pressing need for new therapies with fewer side effects to alleviate cancer pain. We recently developed a topical herbal formula Xiaotan Tongluo analgesic gel (XTTL gel) based on the principles of traditional Chinese herbalism, and we have received positive feedback from bone cancer pain patients. The aim of this study was to determine the analgesic effects and explore the mechanisms of XTTL gel in a rat model of bone cancer pain. Methods: The rat model of bone cancer pain was established by inoculating Walker-256 rat carcinoma cells directly into the right tibial medullary cavity of Wistar rats. The rats were randomly assigned to three groups (n=10 per group): (1) sham bone cancer control (sham group): vehicle (PBS) inoculation without carcinoma cells plus topical administration of blank gel; (2) Sham treatment control (vehicle group): Walker-256 cell inoculation plus topical administration of blank gel; (3) XTTL gel treatment (treatment group): Walker-256 cell inoculation plus topical administration of XTTL gel. XTTL gel treatments were applied daily for 7 days starting on day 14 following inoculation. Outcomes were assessed 21 days after inoculation by mechanical allodynia, histological staining, and by measuring concentrations of type I collagen carboxy-terminal telopeptide (ICTP) and bone-specific alkaline phosphatase (BAP) in serum. Results: Fourteen days after cancer cell incubation, significant mechanical allodynia in the ipsilateral hind paw and tumor growth in proximal end of the tibia were observed in the vehicle and treatment groups but not in the sham group. At day 21, mechanical withdrawal thresholds in treatment group rats were significantly higher ((4.8557±0.8336) g) compared with those of the vehicle group ((1.8630±1.4369) g, P <0.05). ICTP and BAP levels increased significantly in vehicle group rats ((101.5176±11.0694) U/L and (370.7838±12.8273) U/L, respectively) compared with those of the sham group ((11.7553±1.1885) U/L and (185.7338±3.6761) U/L, respectively; P <0.05). XTTL gel decreased the level of blood serum ICTP ((41.8998±6.4970) U/L, P <0.05) but had little effect on blood serum BAP ((365.5338±18.5361) U/L, P <0.05). Conclusion: Topical use of XTTL gel may have an analgesic effect on bone cancer pain, an effect mediated by lowering of ICTP levels and inhibiting bone resorption.
Persistent Identifierhttp://hdl.handle.net/10722/188612
ISSN
2015 Impact Factor: 0.957
2015 SCImago Journal Rankings: 0.428
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorYu, Sen_US
dc.contributor.authorPeng, HDen_US
dc.contributor.authorJu, DWen_US
dc.contributor.authorWei, PKen_US
dc.contributor.authorXu, Len_US
dc.contributor.authorLao, LXen_US
dc.contributor.authorLi, Jen_US
dc.date.accessioned2013-09-03T04:10:37Z-
dc.date.available2013-09-03T04:10:37Z-
dc.date.issued2009en_US
dc.identifier.citationChinese Medical Journal, 2009, v. 122 n. 17, p. 2027-2031en_US
dc.identifier.issn0366-6999en_US
dc.identifier.urihttp://hdl.handle.net/10722/188612-
dc.description.abstractBackground: Pain has a substantial impact on patients' activities and overall quality of life, but current conventional drugs have debilitating side effects, including gastrointestinal disorders. Thus there is a pressing need for new therapies with fewer side effects to alleviate cancer pain. We recently developed a topical herbal formula Xiaotan Tongluo analgesic gel (XTTL gel) based on the principles of traditional Chinese herbalism, and we have received positive feedback from bone cancer pain patients. The aim of this study was to determine the analgesic effects and explore the mechanisms of XTTL gel in a rat model of bone cancer pain. Methods: The rat model of bone cancer pain was established by inoculating Walker-256 rat carcinoma cells directly into the right tibial medullary cavity of Wistar rats. The rats were randomly assigned to three groups (n=10 per group): (1) sham bone cancer control (sham group): vehicle (PBS) inoculation without carcinoma cells plus topical administration of blank gel; (2) Sham treatment control (vehicle group): Walker-256 cell inoculation plus topical administration of blank gel; (3) XTTL gel treatment (treatment group): Walker-256 cell inoculation plus topical administration of XTTL gel. XTTL gel treatments were applied daily for 7 days starting on day 14 following inoculation. Outcomes were assessed 21 days after inoculation by mechanical allodynia, histological staining, and by measuring concentrations of type I collagen carboxy-terminal telopeptide (ICTP) and bone-specific alkaline phosphatase (BAP) in serum. Results: Fourteen days after cancer cell incubation, significant mechanical allodynia in the ipsilateral hind paw and tumor growth in proximal end of the tibia were observed in the vehicle and treatment groups but not in the sham group. At day 21, mechanical withdrawal thresholds in treatment group rats were significantly higher ((4.8557±0.8336) g) compared with those of the vehicle group ((1.8630±1.4369) g, P <0.05). ICTP and BAP levels increased significantly in vehicle group rats ((101.5176±11.0694) U/L and (370.7838±12.8273) U/L, respectively) compared with those of the sham group ((11.7553±1.1885) U/L and (185.7338±3.6761) U/L, respectively; P <0.05). XTTL gel decreased the level of blood serum ICTP ((41.8998±6.4970) U/L, P <0.05) but had little effect on blood serum BAP ((365.5338±18.5361) U/L, P <0.05). Conclusion: Topical use of XTTL gel may have an analgesic effect on bone cancer pain, an effect mediated by lowering of ICTP levels and inhibiting bone resorption.en_US
dc.languageengen_US
dc.publisherChinese Medical Association. The Journal's web site is located at http://www.cmj.org/en_US
dc.relation.ispartofChinese Medical Journalen_US
dc.subject.meshAlkaline Phosphatase - Metabolismen_US
dc.subject.meshAnimalsen_US
dc.subject.meshBody Weighten_US
dc.subject.meshBone Neoplasms - Complicationsen_US
dc.subject.meshCell Line, Tumoren_US
dc.subject.meshCollagen Type Ien_US
dc.subject.meshDrugs, Chinese Herbal - Therapeutic Useen_US
dc.subject.meshFemaleen_US
dc.subject.meshPain - Drug Therapy - Etiologyen_US
dc.subject.meshPeptide Fragments - Blooden_US
dc.subject.meshPeptidesen_US
dc.subject.meshProcollagen - Blooden_US
dc.subject.meshRandom Allocationen_US
dc.subject.meshRatsen_US
dc.subject.meshRats, Wistaren_US
dc.titleMechanisms of treatment of cancer pain with a topical Chinese herbal formula in ratsen_US
dc.typeArticleen_US
dc.identifier.emailLao, LX: lxlao1@hku.hken_US
dc.identifier.authorityLao, LX=rp01784en_US
dc.description.naturelink_to_OA_fulltexten_US
dc.identifier.doi10.3760/cma.j.issn.0366-6999.2009.17.013en_US
dc.identifier.pmid19781391-
dc.identifier.scopuseid_2-s2.0-70350530821en_US
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-70350530821&selection=ref&src=s&origin=recordpageen_US
dc.identifier.volume122en_US
dc.identifier.issue17en_US
dc.identifier.spage2027en_US
dc.identifier.epage2031en_US
dc.identifier.isiWOS:000269783700013-
dc.publisher.placeChinaen_US
dc.identifier.scopusauthoridYu, S=35081457600en_US
dc.identifier.scopusauthoridPeng, HD=36881687200en_US
dc.identifier.scopusauthoridJu, DW=35221522000en_US
dc.identifier.scopusauthoridWei, PK=8976704400en_US
dc.identifier.scopusauthoridXu, L=36524242100en_US
dc.identifier.scopusauthoridLao, LX=7005681883en_US
dc.identifier.scopusauthoridLi, J=7410055320en_US

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