File Download

There are no files associated with this item.

  Links for fulltext
     (May Require Subscription)
Supplementary

Article: Extract of the Chinese herbal formula Huo Luo Xiao Ling Dan inhibited adjuvant arthritis in rats

TitleExtract of the Chinese herbal formula Huo Luo Xiao Ling Dan inhibited adjuvant arthritis in rats
Authors
Issue Date2009
PublisherElsevier Ireland Ltd. The Journal's web site is located at http://www.elsevier.com/locate/jethpharm
Citation
Journal Of Ethnopharmacology, 2009, v. 121 n. 3, p. 366-371 How to Cite?
AbstractEthnopharmacological relevance: The herbal formula Huo Luo Xiao Ling Dan (HLXL) and its modifications have been used in traditional Chinese medicine for about one hundred years to alleviate pain and inflammation. Aim: To investigate the effects of HLXL on complete Freund's adjuvant (CFA)-induced multiple-joint arthritis in rats. Materials and methods: Male Lewis rats, 190-210 g, were immunized subcutaneously at the base of the tail with 200 μl of heat-killed Mycobacterium tuberculosis in mineral oil (5 mg/ml). HLXL (2.30 and 4.60 g/kg) or vehicle control (n = 8 per group) was administered orally (i.g.) once a day between days 16 and 25 post-CFA injection. The rats were observed for signs of arthritis with arthritic changes (erythema, edema, induration) being scored on a scale of 0-4 of increasing severity using a standard scoring system. The maximum arthritis score per rat was 16. A plethysmometer was used to measure edema volume in each paw. Adverse effects of HLXL were monitored by closely observing the animals for unusual behavioral changes. Levels of tumor necrosis factor alpha (TNF-α) and interleukin-1 beta (IL-1β) in local tissue were measured by enzyme-linked immunosorbent assay on day 25 post-CFA. Results: HLXL significantly decreased arthritis scores between days 23-25 in the 2.30 g/kg group and 21-25 in the 4.60 g/kg group (p < 0.05). It reduced paw edema on days 22 and 24 in the 2.30 g/kg group and on days 20, 22 and 24 in the 4.60 g/kg group compared to control (p < 0.05). Local tissue TNF-α and IL-1β levels on day 25 post-CFA injection were significantly (p < 0.05) lower in rats treated with HLXL than in control rats. No observable adverse effects were found. Conclusion: The data suggest that HLXL produces significant anti-arthritic effects that may be mediated by suppressing pro-inflammatory cytokines, and it appears to be safe. © 2008 Elsevier Ireland Ltd. All rights reserved.
Persistent Identifierhttp://hdl.handle.net/10722/188608
ISSN
2015 Impact Factor: 3.055
2015 SCImago Journal Rankings: 1.156
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorZhang, RXen_US
dc.contributor.authorFan, AYen_US
dc.contributor.authorZhou, ANen_US
dc.contributor.authorMoudgil, KDen_US
dc.contributor.authorMa, ZZen_US
dc.contributor.authorLee, DYWen_US
dc.contributor.authorFong, HHSen_US
dc.contributor.authorBerman, BMen_US
dc.contributor.authorLao, Len_US
dc.date.accessioned2013-09-03T04:10:34Z-
dc.date.available2013-09-03T04:10:34Z-
dc.date.issued2009en_US
dc.identifier.citationJournal Of Ethnopharmacology, 2009, v. 121 n. 3, p. 366-371en_US
dc.identifier.issn0378-8741en_US
dc.identifier.urihttp://hdl.handle.net/10722/188608-
dc.description.abstractEthnopharmacological relevance: The herbal formula Huo Luo Xiao Ling Dan (HLXL) and its modifications have been used in traditional Chinese medicine for about one hundred years to alleviate pain and inflammation. Aim: To investigate the effects of HLXL on complete Freund's adjuvant (CFA)-induced multiple-joint arthritis in rats. Materials and methods: Male Lewis rats, 190-210 g, were immunized subcutaneously at the base of the tail with 200 μl of heat-killed Mycobacterium tuberculosis in mineral oil (5 mg/ml). HLXL (2.30 and 4.60 g/kg) or vehicle control (n = 8 per group) was administered orally (i.g.) once a day between days 16 and 25 post-CFA injection. The rats were observed for signs of arthritis with arthritic changes (erythema, edema, induration) being scored on a scale of 0-4 of increasing severity using a standard scoring system. The maximum arthritis score per rat was 16. A plethysmometer was used to measure edema volume in each paw. Adverse effects of HLXL were monitored by closely observing the animals for unusual behavioral changes. Levels of tumor necrosis factor alpha (TNF-α) and interleukin-1 beta (IL-1β) in local tissue were measured by enzyme-linked immunosorbent assay on day 25 post-CFA. Results: HLXL significantly decreased arthritis scores between days 23-25 in the 2.30 g/kg group and 21-25 in the 4.60 g/kg group (p < 0.05). It reduced paw edema on days 22 and 24 in the 2.30 g/kg group and on days 20, 22 and 24 in the 4.60 g/kg group compared to control (p < 0.05). Local tissue TNF-α and IL-1β levels on day 25 post-CFA injection were significantly (p < 0.05) lower in rats treated with HLXL than in control rats. No observable adverse effects were found. Conclusion: The data suggest that HLXL produces significant anti-arthritic effects that may be mediated by suppressing pro-inflammatory cytokines, and it appears to be safe. © 2008 Elsevier Ireland Ltd. All rights reserved.en_US
dc.languageengen_US
dc.publisherElsevier Ireland Ltd. The Journal's web site is located at http://www.elsevier.com/locate/jethpharmen_US
dc.relation.ispartofJournal of Ethnopharmacologyen_US
dc.subject.meshAngiospermsen_US
dc.subject.meshAnimalsen_US
dc.subject.meshArthritis, Experimental - Drug Therapyen_US
dc.subject.meshDrugs, Chinese Herbal - Adverse Effects - Pharmacology - Therapeutic Useen_US
dc.subject.meshEdema - Drug Therapyen_US
dc.subject.meshFooten_US
dc.subject.meshFreund's Adjuvanten_US
dc.subject.meshInterleukin-1Beta - Antagonists & Inhibitorsen_US
dc.subject.meshMaleen_US
dc.subject.meshPhytotherapyen_US
dc.subject.meshPlant Extracts - Adverse Effects - Pharmacology - Therapeutic Useen_US
dc.subject.meshRatsen_US
dc.subject.meshRats, Inbred Lewen_US
dc.subject.meshTumor Necrosis Factor-Alpha - Antagonists & Inhibitorsen_US
dc.titleExtract of the Chinese herbal formula Huo Luo Xiao Ling Dan inhibited adjuvant arthritis in ratsen_US
dc.typeArticleen_US
dc.identifier.emailLao, L: lxlao1@hku.hken_US
dc.identifier.authorityLao, L=rp01784en_US
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.doi10.1016/j.jep.2008.11.018en_US
dc.identifier.pmid19100323-
dc.identifier.scopuseid_2-s2.0-58149270873en_US
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-58149270873&selection=ref&src=s&origin=recordpageen_US
dc.identifier.volume121en_US
dc.identifier.issue3en_US
dc.identifier.spage366en_US
dc.identifier.epage371en_US
dc.identifier.isiWOS:000263393200003-
dc.publisher.placeIrelanden_US
dc.identifier.scopusauthoridZhang, RX=7404864527en_US
dc.identifier.scopusauthoridFan, AY=7005672886en_US
dc.identifier.scopusauthoridZhou, AN=55161019400en_US
dc.identifier.scopusauthoridMoudgil, KD=7003544028en_US
dc.identifier.scopusauthoridMa, ZZ=8709903000en_US
dc.identifier.scopusauthoridLee, DYW=55808069904en_US
dc.identifier.scopusauthoridFong, HHS=34569199300en_US
dc.identifier.scopusauthoridBerman, BM=35458606800en_US
dc.identifier.scopusauthoridLao, L=7005681883en_US

Export via OAI-PMH Interface in XML Formats


OR


Export to Other Non-XML Formats