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Article: Citrate modulation of high-affinity aspartate transport in prostate epithelial cells.

TitleCitrate modulation of high-affinity aspartate transport in prostate epithelial cells.
Authors
Issue Date1993
Citation
Cellular And Molecular Biology, 1993, v. 39 n. 5, p. 515-524 How to Cite?
AbstractProstate secretory epithelial cells have the unique and highly specialized function of accumulating and secreting extraordinarily high levels of citrate. Aspartate is a major four-carbon source of oxaloacetate required for the net synthesis of citrate by these cells. The prostate epithelial cells contain a Na(+)-coupled, high-affinity aspartate transporter which permits the cells to accumulate aspartate from circulation against a large concentration gradient. Because of this unique relationship between aspartate and citrate, it was important to determine if citrate modulated the high-affinity transport of aspartate into prostate epithelial cells. The current studies with freshly prepared prostate epithelial cells obtained from rat ventral prostate demonstrated that citrate exerted two effects on aspartate transport. Physiological levels of extracellular citrate (i.e., equivalent to circulating levels) markedly inhibited (cis-inhibitory effect) aspartate transport. In contrast, intracellular citrate at concentrations associated with normal in situ cells resulted in two levels of stimulation of aspartate transport. A 4-fold increase in aspartate transport occurred when the intracellular citrate was increased up to 500 nmols/g, and an 11-fold increase resulted when the intracellular citrate concentration was elevated to 1800 nmols/g (which is uniquely characteristic of prostate cells). The combined effect of the cis-inhibitory and trans-stimulatory actions of citrate was a consistent 1-2 fold increase in aspartate transport. These studies support the concept that citrate is a physiological regulator of the high-affinity transport of aspartate into prostate secretory epithelial cells in association with the unique and highly specialized function of net citrate production and secretion.
Persistent Identifierhttp://hdl.handle.net/10722/188551
ISSN
2015 Impact Factor: 0.605
2015 SCImago Journal Rankings: 0.381
ISI Accession Number ID

 

DC FieldValueLanguage
dc.contributor.authorCostello, LCen_US
dc.contributor.authorLao, Len_US
dc.contributor.authorFranklin, Ren_US
dc.date.accessioned2013-09-03T04:10:14Z-
dc.date.available2013-09-03T04:10:14Z-
dc.date.issued1993en_US
dc.identifier.citationCellular And Molecular Biology, 1993, v. 39 n. 5, p. 515-524en_US
dc.identifier.issn0145-5680en_US
dc.identifier.urihttp://hdl.handle.net/10722/188551-
dc.description.abstractProstate secretory epithelial cells have the unique and highly specialized function of accumulating and secreting extraordinarily high levels of citrate. Aspartate is a major four-carbon source of oxaloacetate required for the net synthesis of citrate by these cells. The prostate epithelial cells contain a Na(+)-coupled, high-affinity aspartate transporter which permits the cells to accumulate aspartate from circulation against a large concentration gradient. Because of this unique relationship between aspartate and citrate, it was important to determine if citrate modulated the high-affinity transport of aspartate into prostate epithelial cells. The current studies with freshly prepared prostate epithelial cells obtained from rat ventral prostate demonstrated that citrate exerted two effects on aspartate transport. Physiological levels of extracellular citrate (i.e., equivalent to circulating levels) markedly inhibited (cis-inhibitory effect) aspartate transport. In contrast, intracellular citrate at concentrations associated with normal in situ cells resulted in two levels of stimulation of aspartate transport. A 4-fold increase in aspartate transport occurred when the intracellular citrate was increased up to 500 nmols/g, and an 11-fold increase resulted when the intracellular citrate concentration was elevated to 1800 nmols/g (which is uniquely characteristic of prostate cells). The combined effect of the cis-inhibitory and trans-stimulatory actions of citrate was a consistent 1-2 fold increase in aspartate transport. These studies support the concept that citrate is a physiological regulator of the high-affinity transport of aspartate into prostate secretory epithelial cells in association with the unique and highly specialized function of net citrate production and secretion.en_US
dc.languageengen_US
dc.relation.ispartofCellular and Molecular Biologyen_US
dc.subject.meshAnimalsen_US
dc.subject.meshAspartic Acid - Metabolism - Pharmacologyen_US
dc.subject.meshBiological Transport - Drug Effectsen_US
dc.subject.meshCitrates - Pharmacologyen_US
dc.subject.meshEpithelium - Drug Effects - Metabolismen_US
dc.subject.meshGlutamates - Pharmacologyen_US
dc.subject.meshGlutamic Aciden_US
dc.subject.meshKineticsen_US
dc.subject.meshMaleen_US
dc.subject.meshProstate - Metabolismen_US
dc.subject.meshRatsen_US
dc.subject.meshRats, Wistaren_US
dc.titleCitrate modulation of high-affinity aspartate transport in prostate epithelial cells.en_US
dc.typeArticleen_US
dc.identifier.emailLao, L: lxlao1@hku.hken_US
dc.identifier.authorityLao, L=rp01784en_US
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.pmid8104067-
dc.identifier.scopuseid_2-s2.0-0040709091en_US
dc.identifier.volume39en_US
dc.identifier.issue5en_US
dc.identifier.spage515en_US
dc.identifier.epage524en_US
dc.identifier.isiWOS:A1993LR43200007-
dc.identifier.scopusauthoridCostello, LC=24592383900en_US
dc.identifier.scopusauthoridLao, L=7005681883en_US
dc.identifier.scopusauthoridFranklin, R=7201428474en_US

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