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Article: Plasmid-mediated fosfomycin resistance in Escherichia coli isolated from pig.

TitlePlasmid-mediated fosfomycin resistance in Escherichia coli isolated from pig.
Authors
Issue Date2013
PublisherElsevier BV. The Journal's web site is located at http://www.elsevier.com/locate/vetmic
Citation
Veterinary Microbiology, 2013, v. 162 n. 2-4, p. 964-967 How to Cite?
AbstractPrevious studies have reported plasmid-mediated fosA3 among Escherichia coli originating from human and companion animals. In this study, the plasmid, designated pHK23a originating from a multidrug-resistant E. coli isolate recovered from a slaughter pig in December 2008 in Hong Kong, China was sequenced. In conjugation, the plasmid readily transferred to E. coli J53 at high frequencies. It belongs to the narrow host range IncFII incompatibility group and is 73,607bp in length. Sequence alignment showed that pHK23a has a 59.1kb backbone which shares high homology with the prototype R100 plasmid and a 14.5kb variable region. The variable region includes three genes mediating antimicrobial resistance (fosA3, ΔblaTEM-1, blaCTX-M-3), ten mobile genetic elements (four copies of IS26, insA, ΔinsB, ΔTn2, IS1, ΔISEcp1, Δintl1), the tir transfer inhibition protein, the pemI/pemK addiction system and eight ORFs of unknown functions (orf1, orf2, Δorf3, orf20, orf23, orf24, ycdA and ycdB). The three resistance genes were organized in a novel IS26-composite transposon-like structure. In conclusion, this is the first report of fosA3 containing plasmid in an isolate of pig origin. Since IncFII plasmids spread efficiently in Enterobacteriaceae, the detection of fosA3 with blaCTX-M is worrisome and might become a public health concern. © 2012 Elsevier B.V..
Persistent Identifierhttp://hdl.handle.net/10722/186082
ISSN
2015 Impact Factor: 2.564
2015 SCImago Journal Rankings: 1.381
ISI Accession Number ID

 

DC FieldValueLanguage
dc.contributor.authorHo, PL-
dc.contributor.authorChan, J-
dc.contributor.authorLo, WU-
dc.contributor.authorLaw, PYT-
dc.contributor.authorChow, KH-
dc.date.accessioned2013-08-20T11:53:33Z-
dc.date.available2013-08-20T11:53:33Z-
dc.date.issued2013-
dc.identifier.citationVeterinary Microbiology, 2013, v. 162 n. 2-4, p. 964-967-
dc.identifier.issn0378-1135-
dc.identifier.urihttp://hdl.handle.net/10722/186082-
dc.description.abstractPrevious studies have reported plasmid-mediated fosA3 among Escherichia coli originating from human and companion animals. In this study, the plasmid, designated pHK23a originating from a multidrug-resistant E. coli isolate recovered from a slaughter pig in December 2008 in Hong Kong, China was sequenced. In conjugation, the plasmid readily transferred to E. coli J53 at high frequencies. It belongs to the narrow host range IncFII incompatibility group and is 73,607bp in length. Sequence alignment showed that pHK23a has a 59.1kb backbone which shares high homology with the prototype R100 plasmid and a 14.5kb variable region. The variable region includes three genes mediating antimicrobial resistance (fosA3, ΔblaTEM-1, blaCTX-M-3), ten mobile genetic elements (four copies of IS26, insA, ΔinsB, ΔTn2, IS1, ΔISEcp1, Δintl1), the tir transfer inhibition protein, the pemI/pemK addiction system and eight ORFs of unknown functions (orf1, orf2, Δorf3, orf20, orf23, orf24, ycdA and ycdB). The three resistance genes were organized in a novel IS26-composite transposon-like structure. In conclusion, this is the first report of fosA3 containing plasmid in an isolate of pig origin. Since IncFII plasmids spread efficiently in Enterobacteriaceae, the detection of fosA3 with blaCTX-M is worrisome and might become a public health concern. © 2012 Elsevier B.V..-
dc.languageeng-
dc.publisherElsevier BV. The Journal's web site is located at http://www.elsevier.com/locate/vetmic-
dc.relation.ispartofVeterinary Microbiology-
dc.rights© 2013. This manuscript version is made available under the CC-BY-NC-ND 4.0 license http://creativecommons.org/licenses/by-nc-nd/4.0/-
dc.rightsCreative Commons: Attribution 3.0 Hong Kong License-
dc.titlePlasmid-mediated fosfomycin resistance in Escherichia coli isolated from pig.-
dc.typeArticle-
dc.identifier.emailHo, PL: plho@hkucc.hku.hk-
dc.identifier.emailChan, J: chanjane@hku.hk-
dc.identifier.emailLo, WU: stephlo@hku.hk-
dc.identifier.emailLaw, PYT: plaw@hku.hk-
dc.identifier.emailChow, KH: khchowb@hku.hk-
dc.identifier.authorityHo, PL=rp00406-
dc.identifier.authorityChow, KH=rp00370-
dc.description.naturepostprint-
dc.identifier.doi10.1016/j.vetmic.2012.09.023-
dc.identifier.pmid23078751-
dc.identifier.scopuseid_2-s2.0-84872500300-
dc.identifier.hkuros219325-
dc.identifier.volume162-
dc.identifier.issue2-4-
dc.identifier.spage964-
dc.identifier.epage967-
dc.identifier.isiWOS:000315003000079-
dc.publisher.placeNetherlands-

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