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Article: Dissemination of plasmid-mediated fosfomycin resistance fosA3 among multidrug-resistant Escherichia coli from livestock and other animals.

TitleDissemination of plasmid-mediated fosfomycin resistance fosA3 among multidrug-resistant Escherichia coli from livestock and other animals.
Authors
Issue Date2013
PublisherBlackwell Publishing Ltd. The Journal's web site is located at http://www.blackwellpublishing.com/journals/JAM
Citation
Journal of Applied Microbiology, 2013, v. 114 n. 3, p. 695-702 How to Cite?
AbstractAims: To investigate plasmid-mediated fosfomycin resistance related to fosA3 in Escherichia coli isolates collected from different animals in Hong Kong, China, 2008-2010. Methods and Results: In total, 2106 faecal specimens from 210 cattle, 214 pigs, 460 chickens, 398 stray cats, 368 stray dogs and 456 wild rodents were cultured. The faecal colonization rates of fosfomycin-resistant E. coli were as follows: 11·2% in pigs, 8·6% in cattle, 7·3% in chickens, 2·4% in dogs, 0·8% in cats and 1·5% in rodents. The cultures yielded 1693 isolates of which 831 were extended-spectrum β-lactamases (ESBL) producers. Fosfomycin-resistant isolates were more likely than fosfomycin-susceptible isolates to be producers of ESBL and to have resistance to chloramphenicol, ciprofloxacin, cotrimoxazole, gentamicin and tetracycline. Of the 101 fosfomycin-resistant isolates, 97 (96·0%) isolates were fosA3 positive and 94 (93·1%) were blaCTX-M positive. PCR mapping showed that the fosA3-containing regions were flanked by IS26, both upstream and downstream in 81 (83·5%) isolates, and by an upstream blaCTX-M-14-containing transposon-like structure (ΔISEcp1-blaCTX-M-14-ΔIS903 or ISEcp1-IS10 -blaCTX-M-14-ΔIS903) and a downstream IS26 in 14 (14·4%) isolates. For the remaining two isolates, fosA3 was flanked by a downstream IS26 but the upstream part cannot be defined. In a random subset of 18 isolates, fosA3 was carried on transferable plasmids with sizes of 50-200kb and the following replicons: F2:A-B- (n=3), F16:A1:B- (n=2), F24:A-B- (n=1), N (n=1), B/O (n=1) and untypeable (n=3). Significance and Impact of the Study: This study demonstrates the emergence of fosA3-mediated fosfomycin resistance among multidrug-resistant E. coli isolates from various animals. IS26 transposon-like structures might be the main vehicles for dissemination of fosA3. © 2012 The Society for Applied Microbiology.
Persistent Identifierhttp://hdl.handle.net/10722/186081
ISSN
2015 Impact Factor: 2.156
2015 SCImago Journal Rankings: 0.921
ISI Accession Number ID

 

DC FieldValueLanguage
dc.contributor.authorHo, PL-
dc.contributor.authorChan, J-
dc.contributor.authorLo, WU-
dc.contributor.authorLaw, PYT-
dc.contributor.authorLI, Z-
dc.contributor.authorLai, ELY-
dc.contributor.authorChow, KH-
dc.date.accessioned2013-08-20T11:53:32Z-
dc.date.available2013-08-20T11:53:32Z-
dc.date.issued2013-
dc.identifier.citationJournal of Applied Microbiology, 2013, v. 114 n. 3, p. 695-702-
dc.identifier.issn1364-5072-
dc.identifier.urihttp://hdl.handle.net/10722/186081-
dc.description.abstractAims: To investigate plasmid-mediated fosfomycin resistance related to fosA3 in Escherichia coli isolates collected from different animals in Hong Kong, China, 2008-2010. Methods and Results: In total, 2106 faecal specimens from 210 cattle, 214 pigs, 460 chickens, 398 stray cats, 368 stray dogs and 456 wild rodents were cultured. The faecal colonization rates of fosfomycin-resistant E. coli were as follows: 11·2% in pigs, 8·6% in cattle, 7·3% in chickens, 2·4% in dogs, 0·8% in cats and 1·5% in rodents. The cultures yielded 1693 isolates of which 831 were extended-spectrum β-lactamases (ESBL) producers. Fosfomycin-resistant isolates were more likely than fosfomycin-susceptible isolates to be producers of ESBL and to have resistance to chloramphenicol, ciprofloxacin, cotrimoxazole, gentamicin and tetracycline. Of the 101 fosfomycin-resistant isolates, 97 (96·0%) isolates were fosA3 positive and 94 (93·1%) were blaCTX-M positive. PCR mapping showed that the fosA3-containing regions were flanked by IS26, both upstream and downstream in 81 (83·5%) isolates, and by an upstream blaCTX-M-14-containing transposon-like structure (ΔISEcp1-blaCTX-M-14-ΔIS903 or ISEcp1-IS10 -blaCTX-M-14-ΔIS903) and a downstream IS26 in 14 (14·4%) isolates. For the remaining two isolates, fosA3 was flanked by a downstream IS26 but the upstream part cannot be defined. In a random subset of 18 isolates, fosA3 was carried on transferable plasmids with sizes of 50-200kb and the following replicons: F2:A-B- (n=3), F16:A1:B- (n=2), F24:A-B- (n=1), N (n=1), B/O (n=1) and untypeable (n=3). Significance and Impact of the Study: This study demonstrates the emergence of fosA3-mediated fosfomycin resistance among multidrug-resistant E. coli isolates from various animals. IS26 transposon-like structures might be the main vehicles for dissemination of fosA3. © 2012 The Society for Applied Microbiology.-
dc.languageeng-
dc.publisherBlackwell Publishing Ltd. The Journal's web site is located at http://www.blackwellpublishing.com/journals/JAM-
dc.relation.ispartofJournal of Applied Microbiology-
dc.rightsThe definitive version is available at www.blackwell-synergy.com-
dc.titleDissemination of plasmid-mediated fosfomycin resistance fosA3 among multidrug-resistant Escherichia coli from livestock and other animals.-
dc.typeArticle-
dc.identifier.emailHo, PL: plho@hkucc.hku.hk-
dc.identifier.emailChan, J: chanjane@hku.hk-
dc.identifier.emailLo, WU: stephlo@hku.hk-
dc.identifier.emailLaw, PYT: plaw@hku.hk-
dc.identifier.emailLai, ELY: elylai@hku.hk-
dc.identifier.emailChow, KH: khchowb@hku.hk-
dc.identifier.authorityHo, PL=rp00406-
dc.identifier.authorityChow, KH=rp00370-
dc.identifier.doi10.1111/jam.12099-
dc.identifier.pmid23216653-
dc.identifier.scopuseid_2-s2.0-84873986459-
dc.identifier.hkuros219321-
dc.identifier.volume114-
dc.identifier.issue3-
dc.identifier.spage695-
dc.identifier.epage702-
dc.identifier.isiWOS:000315187400011-
dc.publisher.placeUnited Kingdom-

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