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Article: Selective label-free detection of G-quadruplex structure of human telomere by emission spectral changes in visible-and-NIR region under physiological condition through the FRET of a two-component PPE-SO3−–Pt(ii) complex ensemble with Pt⋯Pt, electrostatic and π–π interactions
Title | Selective label-free detection of G-quadruplex structure of human telomere by emission spectral changes in visible-and-NIR region under physiological condition through the FRET of a two-component PPE-SO3−–Pt(ii) complex ensemble with Pt⋯Pt, electrostatic and π–π interactions |
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Authors | |
Issue Date | 2013 |
Publisher | Royal Society of Chemistry. The Journal's web site is located at http://www.rsc.org/publishing/journals/sc/About.asp |
Citation | Chemical Science, 2013, v. 4 n. 1, p. 377-387 How to Cite? |
Abstract | The formation of polymer–metal complex aggregates and the FRET between PPE-SO3− and several water-soluble cationic alkynylplatinum(II) complexes are revealed from UV-vis, steady-state emission and time-resolved emission decay studies. From the Stern–Volmer plots, [Pt{tpy(C6H4CH2NMe3-4)-4′}(C[triple bond, length as m-dash]CC6H5)](OTf)2 (2) is found to be the most efficient quencher of PPE-SO3− at both low and high concentrations. This has been ascribed to its low steric bulkiness and the stronger interactions with PPE-SO3−, and hence the largest association constant with PPE-SO3−, as well as the largest Förster radius, R0, among the complexes studied. The PPE-SO3−–2 ensemble has been employed in the detection of human telomere in aqueous buffer solution (50 mM KH2PO4, pH 6.8) and found to have better selectivity than the ensemble containing [Pt(tpy)(C[triple bond, length as m-dash]CC6H4CH2NMe3-4)](OTf)2 (1), which has a smaller association constant with PPE-SO3− and R0 value than 2. By modulation of the aggregation/deaggregation of the polymer–metal complex aggregates and hence the FRET from the PPE-SO3− donor to the aggregated forms of 2 as acceptor, the PPE-SO3−–2 ensemble has been demonstrated for the sensitive and selective label-free detection of human telomere via the monitoring of emission spectral changes over the visible-NIR region. Ratiometric emission of PPE-SO3−–2 ensemble at 620 and 795 nm has been shown to distinguish the G-quadruplex structure formed by human telomeric DNA from those of other G-quadruplex-forming sequences. |
Persistent Identifier | http://hdl.handle.net/10722/185669 |
ISSN | 2023 Impact Factor: 7.6 2023 SCImago Journal Rankings: 2.333 |
ISI Accession Number ID |
DC Field | Value | Language |
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dc.contributor.author | Chung, CYS | - |
dc.contributor.author | Yam, VWW | - |
dc.date.accessioned | 2013-08-20T11:37:15Z | - |
dc.date.available | 2013-08-20T11:37:15Z | - |
dc.date.issued | 2013 | - |
dc.identifier.citation | Chemical Science, 2013, v. 4 n. 1, p. 377-387 | - |
dc.identifier.issn | 2041-6520 | - |
dc.identifier.uri | http://hdl.handle.net/10722/185669 | - |
dc.description.abstract | The formation of polymer–metal complex aggregates and the FRET between PPE-SO3− and several water-soluble cationic alkynylplatinum(II) complexes are revealed from UV-vis, steady-state emission and time-resolved emission decay studies. From the Stern–Volmer plots, [Pt{tpy(C6H4CH2NMe3-4)-4′}(C[triple bond, length as m-dash]CC6H5)](OTf)2 (2) is found to be the most efficient quencher of PPE-SO3− at both low and high concentrations. This has been ascribed to its low steric bulkiness and the stronger interactions with PPE-SO3−, and hence the largest association constant with PPE-SO3−, as well as the largest Förster radius, R0, among the complexes studied. The PPE-SO3−–2 ensemble has been employed in the detection of human telomere in aqueous buffer solution (50 mM KH2PO4, pH 6.8) and found to have better selectivity than the ensemble containing [Pt(tpy)(C[triple bond, length as m-dash]CC6H4CH2NMe3-4)](OTf)2 (1), which has a smaller association constant with PPE-SO3− and R0 value than 2. By modulation of the aggregation/deaggregation of the polymer–metal complex aggregates and hence the FRET from the PPE-SO3− donor to the aggregated forms of 2 as acceptor, the PPE-SO3−–2 ensemble has been demonstrated for the sensitive and selective label-free detection of human telomere via the monitoring of emission spectral changes over the visible-NIR region. Ratiometric emission of PPE-SO3−–2 ensemble at 620 and 795 nm has been shown to distinguish the G-quadruplex structure formed by human telomeric DNA from those of other G-quadruplex-forming sequences. | - |
dc.language | eng | - |
dc.publisher | Royal Society of Chemistry. The Journal's web site is located at http://www.rsc.org/publishing/journals/sc/About.asp | - |
dc.relation.ispartof | Chemical Science | - |
dc.title | Selective label-free detection of G-quadruplex structure of human telomere by emission spectral changes in visible-and-NIR region under physiological condition through the FRET of a two-component PPE-SO3−–Pt(ii) complex ensemble with Pt⋯Pt, electrostatic and π–π interactions | - |
dc.type | Article | - |
dc.identifier.email | Chung, CYS: cyschung@hku.hk | - |
dc.identifier.email | Yam, VWW: wwyam@hku.hk | - |
dc.identifier.authority | Chung, CYS=rp02672 | - |
dc.identifier.authority | Yam, VWW=rp00822 | - |
dc.description.nature | link_to_OA_fulltext | - |
dc.identifier.doi | 10.1039/C2SC20897K | - |
dc.identifier.scopus | eid_2-s2.0-84874402162 | - |
dc.identifier.hkuros | 219082 | - |
dc.identifier.volume | 4 | - |
dc.identifier.issue | 1 | - |
dc.identifier.spage | 377 | - |
dc.identifier.epage | 387 | - |
dc.identifier.isi | WOS:000311971500044 | - |
dc.publisher.place | United Kingdom | - |
dc.identifier.issnl | 2041-6520 | - |