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Article: Replication and pathogenesis of avian influenza A (H5N1) virus infection in polarised human bronchial and alveolar epithelium

TitleReplication and pathogenesis of avian influenza A (H5N1) virus infection in polarised human bronchial and alveolar epithelium
Authors
Issue Date2013
PublisherHong Kong Academy of Medicine Press. The Journal's web site is located at http://www.hkmj.org.hk
Citation
Hong Kong Medical Journal, 2013, v. 19 n. 3, Suppl. 4, p. 24-28 How to Cite?
Abstract1. In vitro models of polarised human respiratory epithelial cells were established to investigate the tropism and innate host responses of influenza A (H5N1 and H1N1) viruses. 2. Both viruses efficiently infected alveolar epithelial cells of both the apical and basolateral surfaces of the epithelium, whereas release of newly formed virus was mainly from the apical surface of the epithelium. 3. H5N1 virus was a more potent inducer of cytokines and chemokines in alveolar epithelial cells than H1N1 virus. Such chemokines were secreted onto both the apical and basolateral surfaces of the polarised alveolar epithelium. 4. In bronchial epithelium, the H5N1 virus replicated more efficiently and induced a stronger type I interferon response in the undifferentiated NHBE cells than did H1N1 virus. In contrast, in well-differentiated cultures, H5N1 virus replication was less efficient and elicited a lower interferon-beta response than did H1N1 virus. 5. Recombinant virus with vRNPs of a mammalian PB2 and an avian PB1 had the strongest polymerase activities, and replicated better in human cell cultures, especially at a high incubation temperature. These viruses were potent inducers of cytokines and chemokines in primary human alveolar epithelial cells.
Persistent Identifierhttp://hdl.handle.net/10722/185609
ISSN
2015 Impact Factor: 0.887
2015 SCImago Journal Rankings: 0.279

 

DC FieldValueLanguage
dc.contributor.authorChan, MCWen_US
dc.contributor.authorChan, RWYen_US
dc.contributor.authorTsao, GSWen_US
dc.contributor.authorPeiris, JSMen_US
dc.date.accessioned2013-08-20T11:34:17Z-
dc.date.available2013-08-20T11:34:17Z-
dc.date.issued2013en_US
dc.identifier.citationHong Kong Medical Journal, 2013, v. 19 n. 3, Suppl. 4, p. 24-28en_US
dc.identifier.issn1024-2708-
dc.identifier.urihttp://hdl.handle.net/10722/185609-
dc.description.abstract1. In vitro models of polarised human respiratory epithelial cells were established to investigate the tropism and innate host responses of influenza A (H5N1 and H1N1) viruses. 2. Both viruses efficiently infected alveolar epithelial cells of both the apical and basolateral surfaces of the epithelium, whereas release of newly formed virus was mainly from the apical surface of the epithelium. 3. H5N1 virus was a more potent inducer of cytokines and chemokines in alveolar epithelial cells than H1N1 virus. Such chemokines were secreted onto both the apical and basolateral surfaces of the polarised alveolar epithelium. 4. In bronchial epithelium, the H5N1 virus replicated more efficiently and induced a stronger type I interferon response in the undifferentiated NHBE cells than did H1N1 virus. In contrast, in well-differentiated cultures, H5N1 virus replication was less efficient and elicited a lower interferon-beta response than did H1N1 virus. 5. Recombinant virus with vRNPs of a mammalian PB2 and an avian PB1 had the strongest polymerase activities, and replicated better in human cell cultures, especially at a high incubation temperature. These viruses were potent inducers of cytokines and chemokines in primary human alveolar epithelial cells.-
dc.languageengen_US
dc.publisherHong Kong Academy of Medicine Press. The Journal's web site is located at http://www.hkmj.org.hk-
dc.relation.ispartofHong Kong Medical Journalen_US
dc.rightsHong Kong Medical Journal. Copyright © Hong Kong Academy of Medicine Press.-
dc.rightsCreative Commons: Attribution 3.0 Hong Kong License-
dc.subject.meshCytokines - metabolism-
dc.subject.meshInfluenza A Virus, H1N1 Subtype - pathogenicity - physiology-
dc.subject.meshInfluenza A Virus, H5N1 Subtype - pathogenicity - physiology-
dc.subject.meshRespiratory Mucosa - cytology - metabolism - virology-
dc.subject.meshViral Tropism-
dc.titleReplication and pathogenesis of avian influenza A (H5N1) virus infection in polarised human bronchial and alveolar epitheliumen_US
dc.typeArticleen_US
dc.identifier.emailChan, MCW: mchan@hku.hken_US
dc.identifier.emailChan, RWY: reneewy@hku.hken_US
dc.identifier.emailTsao, GSW: gswtsao@hku.hken_US
dc.identifier.emailPeiris, JSM: malik@hkucc.hku.hken_US
dc.identifier.authorityChan, MCW=rp00420en_US
dc.identifier.authorityChan, RWY=rp01596en_US
dc.identifier.authorityTsao, GSW=rp00399en_US
dc.identifier.authorityPeiris, JSM=rp00410en_US
dc.description.naturepublished_or_final_version-
dc.identifier.pmid23775183-
dc.identifier.hkuros217246en_US
dc.identifier.volume19en_US
dc.identifier.issue3, Suppl. 4-
dc.identifier.spage24en_US
dc.identifier.epage28en_US
dc.publisher.placeHong Kong-

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