File Download
  Links for fulltext
     (May Require Subscription)
Supplementary

Article: Coupling of small leucine-rich proteoglycans to hypoxic survival of a progenitor cell-like subpopulation in Rhesus Macaque intervertebral disc

TitleCoupling of small leucine-rich proteoglycans to hypoxic survival of a progenitor cell-like subpopulation in Rhesus Macaque intervertebral disc
Authors
KeywordsAdult Progenitor Cells
Biglycan
Decorin
Hypoxia-Inducible Factor
Intervertebral Disc
Regeneration
Issue Date2013
PublisherElsevier BV. The Journal's web site is located at http://www.elsevier.com/locate/biomaterials
Citation
Biomaterials, 2013, v. 34 n. 28, p. 6548-6558 How to Cite?
AbstractDegeneration of the intervertebral disc (IVD) is a major spinal disorder that associates with neck and back pain. Recent studies of clinical samples and animal models for IVD degeneration have identified cells with multi-potency in the IVD. However, IVD tissue-specific progenitor cells and their niche components are not clear, although degenerated IVD-derived cells possess invitro characteristics of mesenchymal stromal cell (MSCs). Here, we firstly identified the tissue-specific intervertebral disc progenitor cells (DPCs) from healthy Rhesus monkey and report the niche components modulated the survival of DPCs under hypoxia. DPCs possess clonogenicity, multipotency and retain differentiation potential after extended expansion invitro and invivo. In particular, the nucleus pulposus-derived DPCs are sensitive to low oxygen tension and undergo apoptosis under hypoxic conditions due to their inability to induce/stabilize hypoxia-inducible factors (HIF). The presence of small leucine-rich proteoglycans (SLRP), biglycan or decorin, can reduce the susceptibility of DPCs to hypoxia-induced apoptosis via promoting the activation/stabilization of HIF-1α and HIF-2α. As IVD is avascular, we propose SLRPs are niche components of DPCs in IVD homeostasis, providing new insights in progenitor cell biology and niche factors under a hypoxic microenvironment. © 2013 .
Persistent Identifierhttp://hdl.handle.net/10722/185475
ISSN
2015 Impact Factor: 8.387
2015 SCImago Journal Rankings: 3.565
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorHuang, Sen_US
dc.contributor.authorLeung, VYLen_US
dc.contributor.authorLong, Den_US
dc.contributor.authorChan, Den_US
dc.contributor.authorLu, WWen_US
dc.contributor.authorCheung, KMCen_US
dc.contributor.authorZhou, Gen_US
dc.date.accessioned2013-07-30T07:35:31Z-
dc.date.available2013-07-30T07:35:31Z-
dc.date.issued2013en_US
dc.identifier.citationBiomaterials, 2013, v. 34 n. 28, p. 6548-6558en_US
dc.identifier.issn0142-9612en_US
dc.identifier.urihttp://hdl.handle.net/10722/185475-
dc.description.abstractDegeneration of the intervertebral disc (IVD) is a major spinal disorder that associates with neck and back pain. Recent studies of clinical samples and animal models for IVD degeneration have identified cells with multi-potency in the IVD. However, IVD tissue-specific progenitor cells and their niche components are not clear, although degenerated IVD-derived cells possess invitro characteristics of mesenchymal stromal cell (MSCs). Here, we firstly identified the tissue-specific intervertebral disc progenitor cells (DPCs) from healthy Rhesus monkey and report the niche components modulated the survival of DPCs under hypoxia. DPCs possess clonogenicity, multipotency and retain differentiation potential after extended expansion invitro and invivo. In particular, the nucleus pulposus-derived DPCs are sensitive to low oxygen tension and undergo apoptosis under hypoxic conditions due to their inability to induce/stabilize hypoxia-inducible factors (HIF). The presence of small leucine-rich proteoglycans (SLRP), biglycan or decorin, can reduce the susceptibility of DPCs to hypoxia-induced apoptosis via promoting the activation/stabilization of HIF-1α and HIF-2α. As IVD is avascular, we propose SLRPs are niche components of DPCs in IVD homeostasis, providing new insights in progenitor cell biology and niche factors under a hypoxic microenvironment. © 2013 .en_US
dc.languageengen_US
dc.publisherElsevier BV. The Journal's web site is located at http://www.elsevier.com/locate/biomaterialsen_US
dc.relation.ispartofBiomaterialsen_US
dc.rightsNOTICE: this is the author’s version of a work that was accepted for publication in Biomaterials. Changes resulting from the publishing process, such as peer review, editing, corrections, structural formatting, and other quality control mechanisms may not be reflected in this document. Changes may have been made to this work since it was submitted for publication. A definitive version was subsequently published in Biomaterials, 2013, v. 34 n. 28, p. 6548-6558. DOI: 10.1016/j.biomaterials.2013.05.027-
dc.rightsCreative Commons: Attribution 3.0 Hong Kong License-
dc.subjectAdult Progenitor Cellsen_US
dc.subjectBiglycanen_US
dc.subjectDecorinen_US
dc.subjectHypoxia-Inducible Factoren_US
dc.subjectIntervertebral Discen_US
dc.subjectRegenerationen_US
dc.titleCoupling of small leucine-rich proteoglycans to hypoxic survival of a progenitor cell-like subpopulation in Rhesus Macaque intervertebral discen_US
dc.typeArticleen_US
dc.identifier.emailLeung, VYL: vicleung@hku.hken_US
dc.identifier.emailCheung, KMC: cheungmc@hku.hken_US
dc.identifier.emailZhou, G: wormoscz@gmail.comen_US
dc.identifier.authorityLeung, VYL=rp01764en_US
dc.identifier.authorityCheung, KMC=rp00387en_US
dc.identifier.authorityZhou, G=rp00527en_US
dc.description.naturepostprinten_US
dc.identifier.doi10.1016/j.biomaterials.2013.05.027en_US
dc.identifier.pmid23764115-
dc.identifier.scopuseid_2-s2.0-84879459793en_US
dc.identifier.hkuros223971-
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-84879459793&selection=ref&src=s&origin=recordpageen_US
dc.identifier.volume34en_US
dc.identifier.issue28en_US
dc.identifier.spage6548en_US
dc.identifier.epage6558en_US
dc.identifier.isiWOS:000322049200002-
dc.publisher.placeNetherlandsen_US
dc.identifier.scopusauthoridHuang, S=53982847500en_US
dc.identifier.scopusauthoridLeung, VYL=35337438900en_US
dc.identifier.scopusauthoridLong, D=55644349500en_US
dc.identifier.scopusauthoridChan, D=55497141000en_US
dc.identifier.scopusauthoridLu, WW=55758194300en_US
dc.identifier.scopusauthoridCheung, KMC=7402406754en_US
dc.identifier.scopusauthoridZhou, G=23394245100en_US

Export via OAI-PMH Interface in XML Formats


OR


Export to Other Non-XML Formats