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Article: Effects of proteoliposome composition and draw solution types on separation performance of aquaporin-based proteoliposomes: Implications for seawater desalination using aquaporin-based biomimetic membranes

TitleEffects of proteoliposome composition and draw solution types on separation performance of aquaporin-based proteoliposomes: Implications for seawater desalination using aquaporin-based biomimetic membranes
Authors
Issue Date2013
PublisherAmerican Chemical Society. The Journal's web site is located at http://pubs.acs.org/est
Citation
Environmental Science And Technology, 2013, v. 47 n. 3, p. 1496-1503 How to Cite?
AbstractAquaporins are a large family of water transport proteins in cell membranes. Their high water permeability and solute rejection make them potential building blocks for high-performance biomimetic membranes for desalination. In the current study, proteoliposomes were prepared using AquaporinZ from Escherichia coli cells, and their separation properties were characterized by stopped-flow measurements. The current study systematically investigated the effect of proteoliposome composition (lipid type, protein-to-lipid ratio (PLR), and the addition of cholesterol) on water permeability and NaCl retention. Among the various lipids investigated, 1,2-dioleoyl-sn-glycero-3-phosphocholine (DOPC)-based proteoliposomes were found to have excellent osmotic water permeability and NaCl reflection coefficient values. Increasing the PLR of DOPC proteoliposomes up to 1:200 increased their osmotic water permeability. However, further increase in the PLR reduced the osmotic water permeability probably due to the occurrence of defects in the proteoliposomes, whereas the addition of cholesterol improved their osmotic water permeation likely due to defects sealing. The current study also investigated the effect of major dissolved ions in seawater (e.g., Mg 2+ and SO4 2-) on the stability of proteoliposomes, and design criteria for aquaporin-based biomimetic membranes are proposed in the context of desalination. © 2013 American Chemical Society.
Persistent Identifierhttp://hdl.handle.net/10722/185442
ISSN
2015 Impact Factor: 5.393
2015 SCImago Journal Rankings: 2.664
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorZhao, Yen_US
dc.contributor.authorVararattanavech, Aen_US
dc.contributor.authorLi, Xen_US
dc.contributor.authorHélixnielsen, Cen_US
dc.contributor.authorVissing, Ten_US
dc.contributor.authorTorres, Jen_US
dc.contributor.authorWang, Ren_US
dc.contributor.authorFane, AGen_US
dc.contributor.authorTang, CYen_US
dc.date.accessioned2013-07-30T07:32:35Z-
dc.date.available2013-07-30T07:32:35Z-
dc.date.issued2013en_US
dc.identifier.citationEnvironmental Science And Technology, 2013, v. 47 n. 3, p. 1496-1503en_US
dc.identifier.issn0013-936Xen_US
dc.identifier.urihttp://hdl.handle.net/10722/185442-
dc.description.abstractAquaporins are a large family of water transport proteins in cell membranes. Their high water permeability and solute rejection make them potential building blocks for high-performance biomimetic membranes for desalination. In the current study, proteoliposomes were prepared using AquaporinZ from Escherichia coli cells, and their separation properties were characterized by stopped-flow measurements. The current study systematically investigated the effect of proteoliposome composition (lipid type, protein-to-lipid ratio (PLR), and the addition of cholesterol) on water permeability and NaCl retention. Among the various lipids investigated, 1,2-dioleoyl-sn-glycero-3-phosphocholine (DOPC)-based proteoliposomes were found to have excellent osmotic water permeability and NaCl reflection coefficient values. Increasing the PLR of DOPC proteoliposomes up to 1:200 increased their osmotic water permeability. However, further increase in the PLR reduced the osmotic water permeability probably due to the occurrence of defects in the proteoliposomes, whereas the addition of cholesterol improved their osmotic water permeation likely due to defects sealing. The current study also investigated the effect of major dissolved ions in seawater (e.g., Mg 2+ and SO4 2-) on the stability of proteoliposomes, and design criteria for aquaporin-based biomimetic membranes are proposed in the context of desalination. © 2013 American Chemical Society.en_US
dc.languageengen_US
dc.publisherAmerican Chemical Society. The Journal's web site is located at http://pubs.acs.org/esten_US
dc.relation.ispartofEnvironmental Science and Technologyen_US
dc.titleEffects of proteoliposome composition and draw solution types on separation performance of aquaporin-based proteoliposomes: Implications for seawater desalination using aquaporin-based biomimetic membranesen_US
dc.typeArticleen_US
dc.identifier.emailTang, CY: tangc@hku.hken_US
dc.identifier.authorityTang, CY=rp01765en_US
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.doi10.1021/es304306ten_US
dc.identifier.pmid23311686-
dc.identifier.scopuseid_2-s2.0-84873424951en_US
dc.identifier.hkuros231326-
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-84873424951&selection=ref&src=s&origin=recordpageen_US
dc.identifier.volume47en_US
dc.identifier.issue3en_US
dc.identifier.spage1496en_US
dc.identifier.epage1503en_US
dc.identifier.isiWOS:000314675500041-
dc.publisher.placeUnited Statesen_US
dc.identifier.scopusauthoridZhao, Y=55324866000en_US
dc.identifier.scopusauthoridVararattanavech, A=23986850300en_US
dc.identifier.scopusauthoridLi, X=55262333500en_US
dc.identifier.scopusauthoridHélixnielsen, C=55582592800en_US
dc.identifier.scopusauthoridVissing, T=15827033400en_US
dc.identifier.scopusauthoridTorres, J=55323863800en_US
dc.identifier.scopusauthoridWang, R=35081334000en_US
dc.identifier.scopusauthoridFane, AG=35593963600en_US
dc.identifier.scopusauthoridTang, CY=35489259800en_US

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