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Article: Comparative gene expression analysis of the human periodontal ligament in deciduous and permanent teeth

TitleComparative gene expression analysis of the human periodontal ligament in deciduous and permanent teeth
Authors
Issue Date2013
Citation
PLoS One, 2013, v. 8 n. 4, p. e61231 How to Cite?
AbstractThere are histological and functional differences between human deciduous and permanent periodontal ligament (PDL) tissues. The aim of this study was to determine the differences between these two types of tissue at the molecular level by comparing their gene expression patterns. PDL samples were obtained from permanent premolars (n = 38) and anterior deciduous teeth (n = 31) extracted from 40 healthy persons. Comparative cDNA microarray analysis revealed several differences in gene expression between the deciduous and permanent PDL tissues. These findings were verified by qRT-PCR (quantitative reverse-transcription–polymerase chain reaction) analysis, and the areas where genes are expressed were revealed by immunohistochemical staining. The expressions of 21 genes were up-regulated in deciduous relative to PDL tissues, and those of 30 genes were up-regulated in permanent relative to deciduous PDL tissues. The genes that were up-regulated in deciduous PDL tissues were those involved in the formation of the extracellular matrix (LAMC2, LAMB3, and COMP), tissue development (IGF2BP, MAB21L2, and PAX3), and inflammatory or immune reactions leading to tissue degradation (IL1A, CCL21, and CCL18). The up-regulated genes in permanent PDL tissues were related to tissue degradation (IL6 and ADAMTS18), myocontraction (PDE3B, CASQ2, and MYH10), and neurological responses (FOS, NCAM2, SYT1, SLC22A3, DOCK3, LRRTM1, LRRTM3, PRSS12, and ARPP21). The analysis of differential gene expressions between deciduous and permanent PDL tissues aids our understanding of histological and functional differences between them at the molecular level.
Persistent Identifierhttp://hdl.handle.net/10722/184436
PubMed Central ID

 

DC FieldValueLanguage
dc.contributor.authorSong, JSen_US
dc.contributor.authorHwang, DHen_US
dc.contributor.authorKim, SOen_US
dc.contributor.authorJeon, Men_US
dc.contributor.authorChoi, BJen_US
dc.contributor.authorJung, HSen_US
dc.contributor.authorMoon, SJen_US
dc.contributor.authorPark, Wen_US
dc.contributor.authorChoi, HJen_US
dc.date.accessioned2013-07-15T09:45:37Z-
dc.date.available2013-07-15T09:45:37Z-
dc.date.issued2013en_US
dc.identifier.citationPLoS One, 2013, v. 8 n. 4, p. e61231en_US
dc.identifier.urihttp://hdl.handle.net/10722/184436-
dc.description.abstractThere are histological and functional differences between human deciduous and permanent periodontal ligament (PDL) tissues. The aim of this study was to determine the differences between these two types of tissue at the molecular level by comparing their gene expression patterns. PDL samples were obtained from permanent premolars (n = 38) and anterior deciduous teeth (n = 31) extracted from 40 healthy persons. Comparative cDNA microarray analysis revealed several differences in gene expression between the deciduous and permanent PDL tissues. These findings were verified by qRT-PCR (quantitative reverse-transcription–polymerase chain reaction) analysis, and the areas where genes are expressed were revealed by immunohistochemical staining. The expressions of 21 genes were up-regulated in deciduous relative to PDL tissues, and those of 30 genes were up-regulated in permanent relative to deciduous PDL tissues. The genes that were up-regulated in deciduous PDL tissues were those involved in the formation of the extracellular matrix (LAMC2, LAMB3, and COMP), tissue development (IGF2BP, MAB21L2, and PAX3), and inflammatory or immune reactions leading to tissue degradation (IL1A, CCL21, and CCL18). The up-regulated genes in permanent PDL tissues were related to tissue degradation (IL6 and ADAMTS18), myocontraction (PDE3B, CASQ2, and MYH10), and neurological responses (FOS, NCAM2, SYT1, SLC22A3, DOCK3, LRRTM1, LRRTM3, PRSS12, and ARPP21). The analysis of differential gene expressions between deciduous and permanent PDL tissues aids our understanding of histological and functional differences between them at the molecular level.-
dc.languageengen_US
dc.relation.ispartofPLoS Oneen_US
dc.rightsCreative Commons: Attribution 3.0 Hong Kong License-
dc.titleComparative gene expression analysis of the human periodontal ligament in deciduous and permanent teethen_US
dc.typeArticleen_US
dc.identifier.emailJung, HS: hsjung@hku.hken_US
dc.identifier.authorityJung, HS=rp01683en_US
dc.description.naturepublished_or_final_version-
dc.identifier.doi10.1371/journal.pone.0061231-
dc.identifier.pmcidPMC3620385-
dc.identifier.hkuros215774en_US
dc.identifier.volume8en_US
dc.identifier.issue4en_US
dc.identifier.spagee61231en_US
dc.identifier.epagee61231en_US

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