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Article: Mother-to-child transmission and the distribution of HIV-1 genotypes in some regions of China

TitleMother-to-child transmission and the distribution of HIV-1 genotypes in some regions of China
中国部分地区HIV-1流行株基因型分布与母婴传播
Authors
Issue Date2004
PublisherChinese Medical Association [中華醫學會].
Citation
Chinese Journal of Epidemiology, 2004, v. 25 n. 12, p. 1013-1018 How to Cite?
中華流行病學雜誌, 2004, v. 25 n. 12, p. 1013-1018 How to Cite?
AbstractOBJECTIVE: To study the distribution of human immunodeficiency virus (HIV)-1 genotypes in major prevalent regions of China and to illustrate the relationship between HIV-1 subtypes and mother-to-child transmission in a retrospective cohort. METHODS: HIV-1 gag p17 and env C2-V4 region were amplified by nested-polymerase chain reaction (nPCR) and the sequences were obtained by sequencing gag nPCR products or clones of env gene. RESULTS: 60 HIV-1 positive individuals were subject to typing for gag p17 and 69 for env C2-V4 region. Single clade was only found in Henan (subtype B') and Xinjiang (subtype C), and subtypes C and E were demonstrated in Yunnan. These regions represented most of the HIV-1 infections in China. Multiple subtypes (A, B, C, E, etc.) were found in Beijing and Shanghai, where HIV infections were still in low level. The sequences of subtype C were less diversive in Xinjiang (p17: 0.0192 +/- 0.0078, C2-V4: 0.0455 +/- 0.0145) than in Yunnan (p17: 0.0279 +/- 0.0102, C2-V4: 0.0482 +/- 0.0171), but all of them clustered in 'C' branch in phylogenetic trees. Trafficking of subtype C from Yunnan to Xinjiang was found but had already been reported by others. Compared to subtype C, subtype E was quite divergent (p17: 0.0473 +/- 0.0105, C2-V4: 0.1114 +/- 0.0112) in Yunnan, but no recombination was found in the C2-V4 region of env gene. Highe divergence of subtype B' was found in Henan and the peripheral provinces (p17: 0.0381 +/- 0.0101, C2-V4: 0.0691 +/- 0.0166), which might be attributed to the early epidemics of HIV-1 in these areas (early 1990's). In maternal-child cohort, subtypes B (7/21), C (11/21), E (1/21) and undefined types (2/21) were identified in non-transmitting HIV-1 positive mothers, while only subtype B (7/11) and C (4/11) appeared in transmitting HIV-1 positive mothers. The rate of transmission was 53.8% (7/13) in mothers infected with subtype B and 30.8% (4/13) in those infected with subtype C, but with no significant difference (P = 0.196). The imbalancing distribution of subtypes might be explained by the fact that transfusion or illegal blood would increased mother-to-child transmission on HIV-1 and most of mothers with clade B were infected by illegal blood transfusion in this cohort. In addition, most of the maternal-child pair's sequences clustered in gag or env phylogenetic trees but only a few did disperse among the unrelated patients because children were older (>/= 4 years). CONCLUSION: The characteristics of HIV-1 clade's distribution differed over most parts of China but no difference was demonstrated between subtype B and C in mother-to-child transmission on HIV-1.
目的了解中国部分地区艾滋病病毒(HIV)-1主要流行区的病毒基因型分布特征及其对母婴传播的影响.方法通过巢式聚合酶链反应对来自全国11个省(区)的HIV-1阳性病例(包括母婴病例)的gag基因和env基因的部分区域进行扩增并测序,采用DNA分析软件进行系统树和距离等分析.结果共完成gag基因p17测序60例,env基因C2~V4区测序69例.新疆自治区和河南地区的流行株均很单一,前者为C亚型,河南省及周边地区为泰国B亚型(B'),云南地区主要为C和E亚型,而在北京和上海地区有A、B、C、E等多种不同亚型.新疆的病毒株与云南地区的C亚型极为相似,来源相似.在32对母婴病例中,主要为B和C亚型,E亚型1例,未定型2例.B亚型母亲的母婴传播率(50.0%)较明显地高于C亚型(26.7%),但差异无统计学意义.结论中国部分地区HIV-1的亚型分布具有明显的地域特性,亚型对母婴传播的影响尚不清楚.
Persistent Identifierhttp://hdl.handle.net/10722/184305
ISSN
2015 SCImago Journal Rankings: 0.191

 

DC FieldValueLanguage
dc.contributor.authorLi, GH-
dc.contributor.authorChen, Z-
dc.contributor.authorChen, Z-
dc.contributor.authorWei, FL-
dc.contributor.authorMei, S-
dc.contributor.authorHuang, YX-
dc.contributor.authorZhang, L-
dc.contributor.authorCao, YZ-
dc.date.accessioned2013-07-05T08:27:55Z-
dc.date.available2013-07-05T08:27:55Z-
dc.date.issued2004-
dc.identifier.citationChinese Journal of Epidemiology, 2004, v. 25 n. 12, p. 1013-1018-
dc.identifier.citation中華流行病學雜誌, 2004, v. 25 n. 12, p. 1013-1018-
dc.identifier.issn0254-6450-
dc.identifier.urihttp://hdl.handle.net/10722/184305-
dc.description.abstractOBJECTIVE: To study the distribution of human immunodeficiency virus (HIV)-1 genotypes in major prevalent regions of China and to illustrate the relationship between HIV-1 subtypes and mother-to-child transmission in a retrospective cohort. METHODS: HIV-1 gag p17 and env C2-V4 region were amplified by nested-polymerase chain reaction (nPCR) and the sequences were obtained by sequencing gag nPCR products or clones of env gene. RESULTS: 60 HIV-1 positive individuals were subject to typing for gag p17 and 69 for env C2-V4 region. Single clade was only found in Henan (subtype B') and Xinjiang (subtype C), and subtypes C and E were demonstrated in Yunnan. These regions represented most of the HIV-1 infections in China. Multiple subtypes (A, B, C, E, etc.) were found in Beijing and Shanghai, where HIV infections were still in low level. The sequences of subtype C were less diversive in Xinjiang (p17: 0.0192 +/- 0.0078, C2-V4: 0.0455 +/- 0.0145) than in Yunnan (p17: 0.0279 +/- 0.0102, C2-V4: 0.0482 +/- 0.0171), but all of them clustered in 'C' branch in phylogenetic trees. Trafficking of subtype C from Yunnan to Xinjiang was found but had already been reported by others. Compared to subtype C, subtype E was quite divergent (p17: 0.0473 +/- 0.0105, C2-V4: 0.1114 +/- 0.0112) in Yunnan, but no recombination was found in the C2-V4 region of env gene. Highe divergence of subtype B' was found in Henan and the peripheral provinces (p17: 0.0381 +/- 0.0101, C2-V4: 0.0691 +/- 0.0166), which might be attributed to the early epidemics of HIV-1 in these areas (early 1990's). In maternal-child cohort, subtypes B (7/21), C (11/21), E (1/21) and undefined types (2/21) were identified in non-transmitting HIV-1 positive mothers, while only subtype B (7/11) and C (4/11) appeared in transmitting HIV-1 positive mothers. The rate of transmission was 53.8% (7/13) in mothers infected with subtype B and 30.8% (4/13) in those infected with subtype C, but with no significant difference (P = 0.196). The imbalancing distribution of subtypes might be explained by the fact that transfusion or illegal blood would increased mother-to-child transmission on HIV-1 and most of mothers with clade B were infected by illegal blood transfusion in this cohort. In addition, most of the maternal-child pair's sequences clustered in gag or env phylogenetic trees but only a few did disperse among the unrelated patients because children were older (>/= 4 years). CONCLUSION: The characteristics of HIV-1 clade's distribution differed over most parts of China but no difference was demonstrated between subtype B and C in mother-to-child transmission on HIV-1.-
dc.description.abstract目的了解中国部分地区艾滋病病毒(HIV)-1主要流行区的病毒基因型分布特征及其对母婴传播的影响.方法通过巢式聚合酶链反应对来自全国11个省(区)的HIV-1阳性病例(包括母婴病例)的gag基因和env基因的部分区域进行扩增并测序,采用DNA分析软件进行系统树和距离等分析.结果共完成gag基因p17测序60例,env基因C2~V4区测序69例.新疆自治区和河南地区的流行株均很单一,前者为C亚型,河南省及周边地区为泰国B亚型(B'),云南地区主要为C和E亚型,而在北京和上海地区有A、B、C、E等多种不同亚型.新疆的病毒株与云南地区的C亚型极为相似,来源相似.在32对母婴病例中,主要为B和C亚型,E亚型1例,未定型2例.B亚型母亲的母婴传播率(50.0%)较明显地高于C亚型(26.7%),但差异无统计学意义.结论中国部分地区HIV-1的亚型分布具有明显的地域特性,亚型对母婴传播的影响尚不清楚.-
dc.languagechi-
dc.languageeng-
dc.publisherChinese Medical Association [中華醫學會].-
dc.relation.ispartofChinese Journal of Epidemiology-
dc.relation.ispartof中華流行病學雜誌-
dc.subject.meshBlood Transfusion - adverse effects-
dc.subject.meshGenes, gag - genetics-
dc.subject.meshHIV Infections - epidemiology - transmission - virology-
dc.subject.meshHIV-1 - classification - genetics-
dc.subject.meshInfectious Disease Transmission, Vertical-
dc.titleMother-to-child transmission and the distribution of HIV-1 genotypes in some regions of Chinaen_US
dc.title中国部分地区HIV-1流行株基因型分布与母婴传播-
dc.typeArticleen_US
dc.identifier.emailChen, Z: zchenai@hkucc.hku.hk-
dc.identifier.volume25-
dc.identifier.issue12-
dc.identifier.spage1013-
dc.identifier.epage1018-
dc.publisher.placeChina-

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