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Article: Development and characterization of an endometrial tissue culture model for study of early implantation events

TitleDevelopment and characterization of an endometrial tissue culture model for study of early implantation events
Authors
Issue Date2012
PublisherElsevier Inc. The Journal's web site is located at http://www.elsevier.com/locate/fertnstert
Citation
Fertility And Sterility, 2012, v. 98 n. 6, p. 1581-1589 How to Cite?
AbstractObjective: To improve and characterize an endometrial tissue culture model. Design: Experimental study of the characteristics of mouse endometrial tissue cultured on amniotic membrane matrix. Setting: University research laboratory. Animal(s): Sexually mature female ICR mice. Intervention(s): Histologic examination of the cultured endometrial tissues. The attachment rates of the cultured tissues to implantation blastocysts under various conditions were determined. Main Outcome Measure(s): Morphometric analysis of the cultured tissues. Blastocyst attachment rate and expression of decidualization markers cylcooxygenase-2, connexin 43, and peroxisome proliferator-activated receptor δ. Result(s): Endometrial tissues could be grown on amniotic membrane matrix for 3 days with morphometric parameters similar to those in the in vivo pseudopregnant control. The cultured tissues responded to the surrounding steroid environment. Morphometric assessment indicated that medium containing 63.5 nmol/L P and 0.9 nmol/L E2 provided the best support. The condition allowed attachment of approximately 60% of the cocultured blastocysts. A small percentage of the attached blastocyst started to penetrate the luminal epithelium within 28 hours. The attachment rate was significantly reduced with prior treatment of the cultured endometrium with anti-leukemia inhibitory factor antibody. The attached blastocyst induced decidualization around the attachment site. Conclusion(s): The model is useful for the study on implantation in the mouse. © 2012 by American Society for Reproductive Medicine.
Persistent Identifierhttp://hdl.handle.net/10722/184295
ISSN
2015 Impact Factor: 4.426
2015 SCImago Journal Rankings: 2.156
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorYe, TMen_US
dc.contributor.authorPang, RTKen_US
dc.contributor.authorLeung, CONen_US
dc.contributor.authorLiu, Wen_US
dc.contributor.authorYeung, WSBen_US
dc.date.accessioned2013-07-04T06:10:59Z-
dc.date.available2013-07-04T06:10:59Z-
dc.date.issued2012en_US
dc.identifier.citationFertility And Sterility, 2012, v. 98 n. 6, p. 1581-1589en_US
dc.identifier.issn0015-0282en_US
dc.identifier.urihttp://hdl.handle.net/10722/184295-
dc.description.abstractObjective: To improve and characterize an endometrial tissue culture model. Design: Experimental study of the characteristics of mouse endometrial tissue cultured on amniotic membrane matrix. Setting: University research laboratory. Animal(s): Sexually mature female ICR mice. Intervention(s): Histologic examination of the cultured endometrial tissues. The attachment rates of the cultured tissues to implantation blastocysts under various conditions were determined. Main Outcome Measure(s): Morphometric analysis of the cultured tissues. Blastocyst attachment rate and expression of decidualization markers cylcooxygenase-2, connexin 43, and peroxisome proliferator-activated receptor δ. Result(s): Endometrial tissues could be grown on amniotic membrane matrix for 3 days with morphometric parameters similar to those in the in vivo pseudopregnant control. The cultured tissues responded to the surrounding steroid environment. Morphometric assessment indicated that medium containing 63.5 nmol/L P and 0.9 nmol/L E2 provided the best support. The condition allowed attachment of approximately 60% of the cocultured blastocysts. A small percentage of the attached blastocyst started to penetrate the luminal epithelium within 28 hours. The attachment rate was significantly reduced with prior treatment of the cultured endometrium with anti-leukemia inhibitory factor antibody. The attached blastocyst induced decidualization around the attachment site. Conclusion(s): The model is useful for the study on implantation in the mouse. © 2012 by American Society for Reproductive Medicine.en_US
dc.languageengen_US
dc.publisherElsevier Inc. The Journal's web site is located at http://www.elsevier.com/locate/fertnsterten_US
dc.relation.ispartofFertility and Sterilityen_US
dc.subject.meshAmnion - Physiologyen_US
dc.subject.meshAnimalsen_US
dc.subject.meshEmbryo Implantation - Physiologyen_US
dc.subject.meshEmbryo Transfer - Methodsen_US
dc.subject.meshEndometrium - Physiologyen_US
dc.subject.meshFemaleen_US
dc.subject.meshHumansen_US
dc.subject.meshMiceen_US
dc.subject.meshMice, Inbred Icren_US
dc.subject.meshModels, Animalen_US
dc.subject.meshTissue Culture Techniques - Methodsen_US
dc.titleDevelopment and characterization of an endometrial tissue culture model for study of early implantation eventsen_US
dc.typeArticleen_US
dc.identifier.emailPang, RTK: rtkpang@hku.hken_US
dc.identifier.authorityPang, RTK=rp01761en_US
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.doi10.1016/j.fertnstert.2012.08.013en_US
dc.identifier.pmid22963809-
dc.identifier.scopuseid_2-s2.0-84870321782en_US
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-84870321782&selection=ref&src=s&origin=recordpageen_US
dc.identifier.volume98en_US
dc.identifier.issue6en_US
dc.identifier.spage1581en_US
dc.identifier.epage1589en_US
dc.identifier.isiWOS:000311637300040-
dc.publisher.placeUnited Statesen_US
dc.identifier.scopusauthoridYe, TM=36166071700en_US
dc.identifier.scopusauthoridPang, RTK=7004376636en_US
dc.identifier.scopusauthoridLeung, CON=36140510700en_US
dc.identifier.scopusauthoridLiu, W=54682064800en_US
dc.identifier.scopusauthoridYeung, WSB=55763794780en_US

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