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- Publisher Website: 10.1016/j.fertnstert.2012.08.013
- Scopus: eid_2-s2.0-84870321782
- PMID: 22963809
- WOS: WOS:000311637300040
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Article: Development and characterization of an endometrial tissue culture model for study of early implantation events
Title | Development and characterization of an endometrial tissue culture model for study of early implantation events |
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Authors | |
Keywords | Amniotic membrane blastocyst attachment decidualization endometrium steroid |
Issue Date | 2012 |
Publisher | Elsevier Inc. The Journal's web site is located at http://www.elsevier.com/locate/fertnstert |
Citation | Fertility And Sterility, 2012, v. 98 n. 6, p. 1581-1589 How to Cite? |
Abstract | Objective: To improve and characterize an endometrial tissue culture model. Design: Experimental study of the characteristics of mouse endometrial tissue cultured on amniotic membrane matrix. Setting: University research laboratory. Animal(s): Sexually mature female ICR mice. Intervention(s): Histologic examination of the cultured endometrial tissues. The attachment rates of the cultured tissues to implantation blastocysts under various conditions were determined. Main Outcome Measure(s): Morphometric analysis of the cultured tissues. Blastocyst attachment rate and expression of decidualization markers cylcooxygenase-2, connexin 43, and peroxisome proliferator-activated receptor δ. Result(s): Endometrial tissues could be grown on amniotic membrane matrix for 3 days with morphometric parameters similar to those in the in vivo pseudopregnant control. The cultured tissues responded to the surrounding steroid environment. Morphometric assessment indicated that medium containing 63.5 nmol/L P and 0.9 nmol/L E2 provided the best support. The condition allowed attachment of approximately 60% of the cocultured blastocysts. A small percentage of the attached blastocyst started to penetrate the luminal epithelium within 28 hours. The attachment rate was significantly reduced with prior treatment of the cultured endometrium with anti-leukemia inhibitory factor antibody. The attached blastocyst induced decidualization around the attachment site. Conclusion(s): The model is useful for the study on implantation in the mouse. © 2012 by American Society for Reproductive Medicine. |
Persistent Identifier | http://hdl.handle.net/10722/184295 |
ISSN | 2023 Impact Factor: 6.6 2023 SCImago Journal Rankings: 1.858 |
ISI Accession Number ID | |
References |
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Ye, TM | en_US |
dc.contributor.author | Pang, RTK | en_US |
dc.contributor.author | Leung, CON | en_US |
dc.contributor.author | Liu, W | en_US |
dc.contributor.author | Yeung, WSB | en_US |
dc.date.accessioned | 2013-07-04T06:10:59Z | - |
dc.date.available | 2013-07-04T06:10:59Z | - |
dc.date.issued | 2012 | en_US |
dc.identifier.citation | Fertility And Sterility, 2012, v. 98 n. 6, p. 1581-1589 | en_US |
dc.identifier.issn | 0015-0282 | en_US |
dc.identifier.uri | http://hdl.handle.net/10722/184295 | - |
dc.description.abstract | Objective: To improve and characterize an endometrial tissue culture model. Design: Experimental study of the characteristics of mouse endometrial tissue cultured on amniotic membrane matrix. Setting: University research laboratory. Animal(s): Sexually mature female ICR mice. Intervention(s): Histologic examination of the cultured endometrial tissues. The attachment rates of the cultured tissues to implantation blastocysts under various conditions were determined. Main Outcome Measure(s): Morphometric analysis of the cultured tissues. Blastocyst attachment rate and expression of decidualization markers cylcooxygenase-2, connexin 43, and peroxisome proliferator-activated receptor δ. Result(s): Endometrial tissues could be grown on amniotic membrane matrix for 3 days with morphometric parameters similar to those in the in vivo pseudopregnant control. The cultured tissues responded to the surrounding steroid environment. Morphometric assessment indicated that medium containing 63.5 nmol/L P and 0.9 nmol/L E2 provided the best support. The condition allowed attachment of approximately 60% of the cocultured blastocysts. A small percentage of the attached blastocyst started to penetrate the luminal epithelium within 28 hours. The attachment rate was significantly reduced with prior treatment of the cultured endometrium with anti-leukemia inhibitory factor antibody. The attached blastocyst induced decidualization around the attachment site. Conclusion(s): The model is useful for the study on implantation in the mouse. © 2012 by American Society for Reproductive Medicine. | en_US |
dc.language | eng | en_US |
dc.publisher | Elsevier Inc. The Journal's web site is located at http://www.elsevier.com/locate/fertnstert | en_US |
dc.relation.ispartof | Fertility and Sterility | en_US |
dc.subject | Amniotic membrane | - |
dc.subject | blastocyst attachment | - |
dc.subject | decidualization | - |
dc.subject | endometrium | - |
dc.subject | steroid | - |
dc.subject.mesh | Amnion - Physiology | en_US |
dc.subject.mesh | Animals | en_US |
dc.subject.mesh | Embryo Implantation - Physiology | en_US |
dc.subject.mesh | Embryo Transfer - Methods | en_US |
dc.subject.mesh | Endometrium - Physiology | en_US |
dc.subject.mesh | Female | en_US |
dc.subject.mesh | Humans | en_US |
dc.subject.mesh | Mice | en_US |
dc.subject.mesh | Mice, Inbred Icr | en_US |
dc.subject.mesh | Models, Animal | en_US |
dc.subject.mesh | Tissue Culture Techniques - Methods | en_US |
dc.title | Development and characterization of an endometrial tissue culture model for study of early implantation events | en_US |
dc.type | Article | en_US |
dc.identifier.email | Pang, RTK: rtkpang@hku.hk | en_US |
dc.identifier.authority | Pang, RTK=rp01761 | en_US |
dc.description.nature | link_to_subscribed_fulltext | en_US |
dc.identifier.doi | 10.1016/j.fertnstert.2012.08.013 | en_US |
dc.identifier.pmid | 22963809 | - |
dc.identifier.scopus | eid_2-s2.0-84870321782 | en_US |
dc.relation.references | http://www.scopus.com/mlt/select.url?eid=2-s2.0-84870321782&selection=ref&src=s&origin=recordpage | en_US |
dc.identifier.volume | 98 | en_US |
dc.identifier.issue | 6 | en_US |
dc.identifier.spage | 1581 | en_US |
dc.identifier.epage | 1589 | en_US |
dc.identifier.isi | WOS:000311637300040 | - |
dc.publisher.place | United States | en_US |
dc.identifier.scopusauthorid | Ye, TM=36166071700 | en_US |
dc.identifier.scopusauthorid | Pang, RTK=7004376636 | en_US |
dc.identifier.scopusauthorid | Leung, CON=36140510700 | en_US |
dc.identifier.scopusauthorid | Liu, W=54682064800 | en_US |
dc.identifier.scopusauthorid | Yeung, WSB=55763794780 | en_US |
dc.identifier.issnl | 0015-0282 | - |