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Article: Proteomic analysis reveals platelet factor 4 and beta-thromboglobulin as prognostic markers in severe acute respiratory syndrome

TitleProteomic analysis reveals platelet factor 4 and beta-thromboglobulin as prognostic markers in severe acute respiratory syndrome
Authors
Issue Date2012
Citation
Electrophoresis, 2012, v. 33 n. 12, p. 1894-1900 How to Cite?
AbstractPreviously, we reported that proteomic fingerprints were present in sera of patients with severe acute respiratory syndrome (SARS), and could separate patients into subgroups with different prognoses. In the present study, we examined the prognostic values of the SARS-associated proteomic features by biostatistical analysis, and deciphered the identities of those with prognostic values. Data of 20 SARS-associated serum proteomic features and ten serological variables from 38 SARS adult patients before treatment were subjected to multivariate logistic regression. Proteomic features of m/z 6634, m/z 7769, m/z 8635, and m/z 8865 were identified as independent prognostic markers. After purification by cation-exchange chromatography and gel electrophoresis, proteomic features of m/z 7769 and m/z 8865 were found to be platelet factor 4 (PF4) and beta-thromboglobulin (beta-TG) by tandem mass spectrometry, respectively. The associations of decreased serum PF4 and increased serum beta-TG levels with poor prognosis were confirmed by Western blot. Previous studies suggest that PF4 and beta-TG are involved in the pathogenesis of acute respiratory distress syndrome (ARDS) in a negative and positive way, respectively. Our results suggest that PF4 and beta-TG may also play similar roles in the development of ARDS in SARS patients. © 2012 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.
Persistent Identifierhttp://hdl.handle.net/10722/184294
ISSN
2015 Impact Factor: 2.482
2015 SCImago Journal Rankings: 0.896
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorPoon, TCen_US
dc.contributor.authorPang, RTen_US
dc.contributor.authorChan, KAen_US
dc.contributor.authorLee, NLen_US
dc.contributor.authorChiu, RWen_US
dc.contributor.authorTong, YKen_US
dc.contributor.authorChim, SSen_US
dc.contributor.authorNgai, SMen_US
dc.contributor.authorSung, JJen_US
dc.contributor.authorLo, YMDen_US
dc.date.accessioned2013-07-04T06:10:58Z-
dc.date.available2013-07-04T06:10:58Z-
dc.date.issued2012en_US
dc.identifier.citationElectrophoresis, 2012, v. 33 n. 12, p. 1894-1900en_US
dc.identifier.issn0173-0835en_US
dc.identifier.urihttp://hdl.handle.net/10722/184294-
dc.description.abstractPreviously, we reported that proteomic fingerprints were present in sera of patients with severe acute respiratory syndrome (SARS), and could separate patients into subgroups with different prognoses. In the present study, we examined the prognostic values of the SARS-associated proteomic features by biostatistical analysis, and deciphered the identities of those with prognostic values. Data of 20 SARS-associated serum proteomic features and ten serological variables from 38 SARS adult patients before treatment were subjected to multivariate logistic regression. Proteomic features of m/z 6634, m/z 7769, m/z 8635, and m/z 8865 were identified as independent prognostic markers. After purification by cation-exchange chromatography and gel electrophoresis, proteomic features of m/z 7769 and m/z 8865 were found to be platelet factor 4 (PF4) and beta-thromboglobulin (beta-TG) by tandem mass spectrometry, respectively. The associations of decreased serum PF4 and increased serum beta-TG levels with poor prognosis were confirmed by Western blot. Previous studies suggest that PF4 and beta-TG are involved in the pathogenesis of acute respiratory distress syndrome (ARDS) in a negative and positive way, respectively. Our results suggest that PF4 and beta-TG may also play similar roles in the development of ARDS in SARS patients. © 2012 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.en_US
dc.languageengen_US
dc.relation.ispartofElectrophoresisen_US
dc.subject.meshAdulten_US
dc.subject.meshAgeden_US
dc.subject.meshAged, 80 And Overen_US
dc.subject.meshBiological Markers - Blooden_US
dc.subject.meshBlotting, Westernen_US
dc.subject.meshFemaleen_US
dc.subject.meshHumansen_US
dc.subject.meshLogistic Modelsen_US
dc.subject.meshMaleen_US
dc.subject.meshMiddle Ageden_US
dc.subject.meshPlatelet Factor 4 - Blooden_US
dc.subject.meshPrognosisen_US
dc.subject.meshProtein Array Analysisen_US
dc.subject.meshProteome - Analysis - Chemistryen_US
dc.subject.meshReproducibility Of Resultsen_US
dc.subject.meshSevere Acute Respiratory Syndrome - Blooden_US
dc.subject.meshSpectrometry, Mass, Matrix-Assisted Laser Desorption-Ionizationen_US
dc.subject.meshBeta-Thromboglobulin - Analysisen_US
dc.titleProteomic analysis reveals platelet factor 4 and beta-thromboglobulin as prognostic markers in severe acute respiratory syndromeen_US
dc.typeArticleen_US
dc.identifier.emailPang, RT: rtkpang@hku.hken_US
dc.identifier.authorityPang, RT=rp01761en_US
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.doi10.1002/elps.201200002en_US
dc.identifier.pmid22740477-
dc.identifier.scopuseid_2-s2.0-84862982234en_US
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-84862982234&selection=ref&src=s&origin=recordpageen_US
dc.identifier.volume33en_US
dc.identifier.issue12en_US
dc.identifier.spage1894en_US
dc.identifier.epage1900en_US
dc.identifier.isiWOS:000305792500026-
dc.publisher.placeGermanyen_US
dc.identifier.scopusauthoridPoon, TC=7006151710en_US
dc.identifier.scopusauthoridPang, RT=7004376636en_US
dc.identifier.scopusauthoridChan, KA=13403797200en_US
dc.identifier.scopusauthoridLee, NL=7402722286en_US
dc.identifier.scopusauthoridChiu, RW=7103038413en_US
dc.identifier.scopusauthoridTong, YK=7202614141en_US
dc.identifier.scopusauthoridChim, SS=6701728226en_US
dc.identifier.scopusauthoridNgai, SM=7006074219en_US
dc.identifier.scopusauthoridSung, JJ=35405352400en_US
dc.identifier.scopusauthoridLo, YMD=7401935391en_US

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