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Article: Effects of TCDD in modulating the expression of Sertoli cell secretory products and markers for cell-cell interaction

TitleEffects of TCDD in modulating the expression of Sertoli cell secretory products and markers for cell-cell interaction
Authors
Issue Date2005
PublisherElsevier Ireland Ltd. The Journal's web site is located at http://www.elsevier.com/locate/toxicol
Citation
Toxicology, 2005, v. 206 n. 1, p. 111-123 How to Cite?
AbstractAmong different mammalian tissues, testis is found to be one of the most sensitive organs to TCDD exposure. In this study, primary Sertoli cell culture was established. The purity of the cultured cells was verified using 3β-hydroxysteroid dehydrogenase, alkaline phosphatase as well as testosterone induction assays. Effects of TCDD in modulating the expression of CYP1A1, aromatase, secretory products (i.e. Müllerian inhibiting substance (MIS), 17β-estradiol (E 2) and lactate) and markers for cell-cell interaction (i.e. sertolin and testin) were then examined. Our data demonstrated that Sertoli cells exposed to 0.2-2000 pg/ml of TCDD showed a dose dependent induction of CYP1A1 mRNA. The minimal dose of activation was 2 pg/ml, which indicated that the cell was very sensitive to TCDD exposure. However, there was little or no detectable level CYP1A1 protein and EROD activities found. Dose-dependent inductions of aromatase transcript (200%) and E 2 (20%) secretion were measured. In addition there was a significant reduction (40%) of MIS mRNA. No detectable change in the level of secreted lactate was observed. Sertolin and testin, the gene makers for cell-cell interactions were differentially modulated upon TCDD treatment. Taken together, the results implicated that TCDD exposure might interfere with the normal Sertoli cell functions. © 2004 Elsevier Ireland Ltd. All rights reserved.
Persistent Identifierhttp://hdl.handle.net/10722/183390
ISSN
2015 Impact Factor: 3.817
2015 SCImago Journal Rankings: 1.335
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorLai, KPen_US
dc.contributor.authorWong, MHen_US
dc.contributor.authorWong, CKCen_US
dc.date.accessioned2013-05-27T07:12:29Z-
dc.date.available2013-05-27T07:12:29Z-
dc.date.issued2005en_US
dc.identifier.citationToxicology, 2005, v. 206 n. 1, p. 111-123en_US
dc.identifier.issn0300-483Xen_US
dc.identifier.urihttp://hdl.handle.net/10722/183390-
dc.description.abstractAmong different mammalian tissues, testis is found to be one of the most sensitive organs to TCDD exposure. In this study, primary Sertoli cell culture was established. The purity of the cultured cells was verified using 3β-hydroxysteroid dehydrogenase, alkaline phosphatase as well as testosterone induction assays. Effects of TCDD in modulating the expression of CYP1A1, aromatase, secretory products (i.e. Müllerian inhibiting substance (MIS), 17β-estradiol (E 2) and lactate) and markers for cell-cell interaction (i.e. sertolin and testin) were then examined. Our data demonstrated that Sertoli cells exposed to 0.2-2000 pg/ml of TCDD showed a dose dependent induction of CYP1A1 mRNA. The minimal dose of activation was 2 pg/ml, which indicated that the cell was very sensitive to TCDD exposure. However, there was little or no detectable level CYP1A1 protein and EROD activities found. Dose-dependent inductions of aromatase transcript (200%) and E 2 (20%) secretion were measured. In addition there was a significant reduction (40%) of MIS mRNA. No detectable change in the level of secreted lactate was observed. Sertolin and testin, the gene makers for cell-cell interactions were differentially modulated upon TCDD treatment. Taken together, the results implicated that TCDD exposure might interfere with the normal Sertoli cell functions. © 2004 Elsevier Ireland Ltd. All rights reserved.en_US
dc.languageengen_US
dc.publisherElsevier Ireland Ltd. The Journal's web site is located at http://www.elsevier.com/locate/toxicolen_US
dc.relation.ispartofToxicologyen_US
dc.subject.meshAnimalsen_US
dc.subject.meshAnti-Mullerian Hormoneen_US
dc.subject.meshAromatase - Biosynthesisen_US
dc.subject.meshBlotting, Westernen_US
dc.subject.meshCell Communication - Drug Effectsen_US
dc.subject.meshCell Survival - Drug Effectsen_US
dc.subject.meshCells, Cultureden_US
dc.subject.meshCytochrome P-450 Cyp1a1 - Biosynthesisen_US
dc.subject.meshDose-Response Relationship, Drugen_US
dc.subject.meshEnvironmental Pollutants - Toxicityen_US
dc.subject.meshEstradiol - Biosynthesisen_US
dc.subject.meshGlycoproteins - Biosynthesisen_US
dc.subject.meshLactic Acid - Biosynthesisen_US
dc.subject.meshMaleen_US
dc.subject.meshPeptides - Secretionen_US
dc.subject.meshProteins - Secretionen_US
dc.subject.meshRatsen_US
dc.subject.meshRats, Sprague-Dawleyen_US
dc.subject.meshReverse Transcriptase Polymerase Chain Reactionen_US
dc.subject.meshSertoli Cells - Drug Effects - Secretionen_US
dc.subject.meshTesticular Hormones - Biosynthesisen_US
dc.subject.meshTetrachlorodibenzodioxin - Toxicityen_US
dc.titleEffects of TCDD in modulating the expression of Sertoli cell secretory products and markers for cell-cell interactionen_US
dc.typeArticleen_US
dc.identifier.emailLai, KP: ballllai@hotmail.comen_US
dc.identifier.authorityLai, KP=rp01753en_US
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.doi10.1016/j.tox.2004.07.002en_US
dc.identifier.pmid15590112-
dc.identifier.scopuseid_2-s2.0-10044243819en_US
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-10044243819&selection=ref&src=s&origin=recordpageen_US
dc.identifier.volume206en_US
dc.identifier.issue1en_US
dc.identifier.spage111en_US
dc.identifier.epage123en_US
dc.identifier.isiWOS:000226439500008-
dc.publisher.placeIrelanden_US
dc.identifier.scopusauthoridLai, KP=7402135707en_US
dc.identifier.scopusauthoridWong, MH=7403908633en_US
dc.identifier.scopusauthoridWong, CKC=35276549400en_US

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