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Article: Changes in endogenous Zn and Cu distribution in different cytosolic protein fractions in mouse liver after administration of a single sublethal dose of CdCl2

TitleChanges in endogenous Zn and Cu distribution in different cytosolic protein fractions in mouse liver after administration of a single sublethal dose of CdCl2
Authors
Issue Date2000
PublisherElsevier Ireland Ltd. The Journal's web site is located at http://www.elsevier.com/locate/toxicol
Citation
Toxicology, 2000, v. 154 n. 1-3, p. 103-111 How to Cite?
AbstractThe time course of change in tissue Cd, Cu and Zn contents, their distribution in cellular protein fractions as well as the profile of MT gene expression in mouse liver was described over a 7 days period following a single intraperitoneal injection of 2 mg/kg of CdCl2. The result showed that Cd accumulated rapidly in mouse liver. Between 1 h and 7 days after administration, over 18% of the total Cd administered were found in the liver. Cd administration was also associated with the overexpression of the MT-mRNA. However, the time course of induction was not parallel to the change in tissue Cd content. When separated on a Sephadex G-75 column, majority of Cd was found to bind to the fractions known to contain the metal-binding protein, metallothionein (MT). From day 2 after Cd administration, a small amount of the metal was also found associated with the high molecular weight (HMW) proteins. In addition to Cd, tissue Zn content was affected most during the entire study. There was a significant decrease in tissue Zn content during the initial 8 h but tissue Zn content increased significantly throughout the following 6 days. At 1-7 days, majority of Zn was associated with the HMW protein fraction. Although there was no significant change in total tissue Cu content, distribution of Cu in different protein fractions was detected. While in control aniamls, Cu was mainly associated with the HMW proteins, some was found in the MT fraction on the second day. On the 7th day, Cu distribution had deteriorated. Together with changes seen in Cd, the results might suggest that injury had occurred in the tissue at this time. The results of the present study showed that Cd caused a change in subcellular distribution of tissue endogenous metals, which might reflect alteration of cellular functional activities. (C) 2000 Elsevier Science Ireland Ltd.
Persistent Identifierhttp://hdl.handle.net/10722/183389
ISSN
2015 Impact Factor: 3.817
2015 SCImago Journal Rankings: 1.335
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorYang, MSen_US
dc.contributor.authorLai, KPen_US
dc.contributor.authorCheng, KYen_US
dc.contributor.authorWong, CKCen_US
dc.date.accessioned2013-05-27T07:12:29Z-
dc.date.available2013-05-27T07:12:29Z-
dc.date.issued2000en_US
dc.identifier.citationToxicology, 2000, v. 154 n. 1-3, p. 103-111en_US
dc.identifier.issn0300-483Xen_US
dc.identifier.urihttp://hdl.handle.net/10722/183389-
dc.description.abstractThe time course of change in tissue Cd, Cu and Zn contents, their distribution in cellular protein fractions as well as the profile of MT gene expression in mouse liver was described over a 7 days period following a single intraperitoneal injection of 2 mg/kg of CdCl2. The result showed that Cd accumulated rapidly in mouse liver. Between 1 h and 7 days after administration, over 18% of the total Cd administered were found in the liver. Cd administration was also associated with the overexpression of the MT-mRNA. However, the time course of induction was not parallel to the change in tissue Cd content. When separated on a Sephadex G-75 column, majority of Cd was found to bind to the fractions known to contain the metal-binding protein, metallothionein (MT). From day 2 after Cd administration, a small amount of the metal was also found associated with the high molecular weight (HMW) proteins. In addition to Cd, tissue Zn content was affected most during the entire study. There was a significant decrease in tissue Zn content during the initial 8 h but tissue Zn content increased significantly throughout the following 6 days. At 1-7 days, majority of Zn was associated with the HMW protein fraction. Although there was no significant change in total tissue Cu content, distribution of Cu in different protein fractions was detected. While in control aniamls, Cu was mainly associated with the HMW proteins, some was found in the MT fraction on the second day. On the 7th day, Cu distribution had deteriorated. Together with changes seen in Cd, the results might suggest that injury had occurred in the tissue at this time. The results of the present study showed that Cd caused a change in subcellular distribution of tissue endogenous metals, which might reflect alteration of cellular functional activities. (C) 2000 Elsevier Science Ireland Ltd.en_US
dc.languageengen_US
dc.publisherElsevier Ireland Ltd. The Journal's web site is located at http://www.elsevier.com/locate/toxicolen_US
dc.relation.ispartofToxicologyen_US
dc.subject.meshAnimalsen_US
dc.subject.meshBlotting, Northernen_US
dc.subject.meshCadmium Chloride - Administration & Dosage - Analysis - Toxicityen_US
dc.subject.meshChromatographyen_US
dc.subject.meshCopper - Analysisen_US
dc.subject.meshCytosol - Chemistry - Drug Effectsen_US
dc.subject.meshDna Probes - Chemistryen_US
dc.subject.meshGene Expression Regulationen_US
dc.subject.meshHomeostasis - Drug Effectsen_US
dc.subject.meshLiver - Chemistry - Drug Effectsen_US
dc.subject.meshMaleen_US
dc.subject.meshMetallothionein - Biosynthesis - Geneticsen_US
dc.subject.meshMiceen_US
dc.subject.meshMice, Inbred Icren_US
dc.subject.meshPolymerase Chain Reactionen_US
dc.subject.meshRna - Chemistry - Isolation & Purificationen_US
dc.subject.meshSequence Analysis, Dnaen_US
dc.subject.meshSpectrophotometry, Atomicen_US
dc.subject.meshZinc - Analysisen_US
dc.titleChanges in endogenous Zn and Cu distribution in different cytosolic protein fractions in mouse liver after administration of a single sublethal dose of CdCl2en_US
dc.typeArticleen_US
dc.identifier.emailLai, KP: ballllai@hotmail.comen_US
dc.identifier.authorityLai, KP=rp01753en_US
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.doi10.1016/S0300-483X(00)00320-6en_US
dc.identifier.pmid11118674-
dc.identifier.scopuseid_2-s2.0-0034706926en_US
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-0034706926&selection=ref&src=s&origin=recordpageen_US
dc.identifier.volume154en_US
dc.identifier.issue1-3en_US
dc.identifier.spage103en_US
dc.identifier.epage111en_US
dc.identifier.isiWOS:000165782900010-
dc.publisher.placeIrelanden_US
dc.identifier.scopusauthoridYang, MS=8105858700en_US
dc.identifier.scopusauthoridLai, KP=7402135707en_US
dc.identifier.scopusauthoridCheng, KY=36986611400en_US
dc.identifier.scopusauthoridWong, CKC=35276549400en_US

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