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Book Chapter: Western Blot Evaluation of siRNA Delivery by pH-Responsive Peptides

TitleWestern Blot Evaluation of siRNA Delivery by pH-Responsive Peptides
Authors
Issue Date2013
PublisherHumana: Springer Science+Business Media
Citation
Western Blot Evaluation of siRNA Delivery by pH-Responsive Peptides. In Moll, J & Colombo, R (Eds.), Target identification and validation in drug discovery: methods and protocols , p. 73-87. New York: Humana: Springer Science+Business Media, 2013 How to Cite?
AbstractGene silencing, via RNA interference (RNAi) technologies using small interfering RNA (siRNA), has been developed as an important tool for target identification and validation in drug discovery and has huge therapeutic potential. However, effective delivery into cells presents a major challenge to the use of siRNA. pH-responsive cell-penetrating peptides have attracted considerable attention in recent years as delivery vectors due to their ability to transport their cargos across the biological membrane and/or to promote endosomal escape and prevent lysosomal degradation. To evaluate the in vitro transfection efficiency of the pH-responsive peptide-based siRNA delivery system, the western blotting technique is commonly employed. This method offers a simple, efficient and economical way to study the gene silencing effect of the siRNA by analysing the protein of interest in a sample with minimum equipment requirement. This chapter provides a description of siRNA delivery and analysis by western blotting protocols for qualitatively and quantitatively assessing gene silencing efficiency and selectivity.
Persistent Identifierhttp://hdl.handle.net/10722/183287
ISBN
Series/Report no.Methods in molecular biology; v. 986

 

DC FieldValueLanguage
dc.contributor.authorLiang, Wen_US
dc.contributor.authorMason, AJen_US
dc.contributor.authorLam, JKWen_US
dc.date.accessioned2013-05-15T01:55:51Z-
dc.date.available2013-05-15T01:55:51Z-
dc.date.issued2013en_US
dc.identifier.citationWestern Blot Evaluation of siRNA Delivery by pH-Responsive Peptides. In Moll, J & Colombo, R (Eds.), Target identification and validation in drug discovery: methods and protocols , p. 73-87. New York: Humana: Springer Science+Business Media, 2013en_US
dc.identifier.isbn9781627033107en_US
dc.identifier.urihttp://hdl.handle.net/10722/183287-
dc.description.abstractGene silencing, via RNA interference (RNAi) technologies using small interfering RNA (siRNA), has been developed as an important tool for target identification and validation in drug discovery and has huge therapeutic potential. However, effective delivery into cells presents a major challenge to the use of siRNA. pH-responsive cell-penetrating peptides have attracted considerable attention in recent years as delivery vectors due to their ability to transport their cargos across the biological membrane and/or to promote endosomal escape and prevent lysosomal degradation. To evaluate the in vitro transfection efficiency of the pH-responsive peptide-based siRNA delivery system, the western blotting technique is commonly employed. This method offers a simple, efficient and economical way to study the gene silencing effect of the siRNA by analysing the protein of interest in a sample with minimum equipment requirement. This chapter provides a description of siRNA delivery and analysis by western blotting protocols for qualitatively and quantitatively assessing gene silencing efficiency and selectivity.-
dc.languageengen_US
dc.publisherHumana: Springer Science+Business Mediaen_US
dc.relation.ispartofTarget identification and validation in drug discovery: methods and protocolsen_US
dc.relation.ispartofseriesMethods in molecular biology; v. 986-
dc.titleWestern Blot Evaluation of siRNA Delivery by pH-Responsive Peptidesen_US
dc.typeBook_Chapteren_US
dc.identifier.emailLam, JKW: jkwlam@hku.hken_US
dc.identifier.authorityLam, JKW=rp01346en_US
dc.identifier.doi10.1007/978-1-62703-311-4_5-
dc.identifier.hkuros214183en_US
dc.identifier.spage73en_US
dc.identifier.epage87en_US
dc.publisher.placeNew York-

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